Investigation of Serotonin 2A Receptor Gene 5-HTR2A rs6311 Polymorphism in Trigeminal Neuralgia Disease

Yıl 2024, Cilt: 8 Sayı: 1, 26 - 30, 30.04.2024

Firuze Çınar , Tolga Polat , Fatma Güngör , Beste Tacal Aslan , Gühan Dergin , Korkut Ulucan , Şehkar Oktay

Öz

Objectives: Trigeminal neuralgia (TN) is a disease that occurs in the distribution region of the trigeminal nerve and is frequently recurrent pain. Serotonin (5-HT) acts on neurotransmitters in the central nervous system. The study aims to examine the relationship between sensitivity and pain intensity of the serotonin receptor gene (5-HTR2A) and rs6311 polymorphism in patients by examining TN genetically.
Materials and Methods: The study consisted of 10 TN patients and 10 healthy individuals. Genotyping of 5-HTR2A rs6311 polymorphism using DNA isolated from blood, Real-Time PCR in StepOnePlus device, and Taqman SNP Genotyping Tests were performed by manufacturers' protocols.
Results: In 5HTR2A rs6311 analysis, 3 of 10 TN patients were found to have CC, 5 had CT and 2 had TT genotype. In the control group, CC genotype was detected in 4 individuals, CT genotype in 4 individuals, and TT genotype in 2 individuals.
Conclusion: It was found that the TN genotype and allelic frequency differences between the patient and control groups were not significant. However, the CT genotype was found to be more common.

Anahtar Kelimeler

Trigeminal neuralgia, polymorphism, serotonin, serotonin receptor

Destekleyen Kurum

This work has been supported by Marmara University Scientific Research Projects Coordination Unit.

Proje Numarası

Grant number TYL-2022-10427

Trigeminal Nevralji Hastalığında Serotonin 2A Reseptör Geni 5-HTR2A rs6311 Polimorfizminin Araştırılması

Öz

Amaç: Trigeminal nevralji (TN), trigeminal sinirin dağılım bölgesinde ortaya çıkan ve sıklıkla tekrarlayan ağrılarla karakterize bir hastalıktır. Serotonin (5-HT), merkezi sinir sistemindeki nörotransmitterlere etki eder. Çalışmada, TN'yi genetik olarak inceleyerek hastalarda serotonin reseptör geni (5-HTR2A) duyarlılığı ve ağrı şiddeti ile rs6311 polimorfizmi arasındaki ilişkiyi araştırmak amaçlanmıştır.
Gereç ve Yöntem: Çalışmada 10 TN hastası ve 10 sağlıklı birey yer almıştır. Kanlardan izole edilen DNA, StepOnePlus cihazında Real-Time PCR ve Taqman SNP Genotipleme Testleri kullanılarak 5-HTR2A rs6311 polimorfizminin genotiplenmesi üretici protokollerine göre yapıldı.
Bulgular: 5HTR2A rs6311 analizinde, 10 TN hastasından 3'ünde CC, 5'inde CT ve 2'sinde TT genotipinin varlığı tespit edildi. Kontrol grubunda ise 4 kişide CC genotipi, 4 kişide CT genotipi ve 2 kişide TT genotipi tespit edildi.
Sonuç: Hasta ve kontrol grupları arasındaki TN genotipi frekans farklılıklarının anlamlı olmadığı görüldü. Ancak CT genotipinin diğerlerine göre daha yaygın olduğu görüldü.

Anahtar Kelimeler

Trigeminal nevralji, polimorfizm, serotonin, serotonin reseptörü

Proje Numarası

Grant number TYL-2022-10427

Kaynakça

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