DEVELOPMENT OF AN ELECTROCHEMICALLY EXFOLIATED GRAPHENE OXIDE MODIFIED ELECTRODE FOR SENSITIVE CHLORAMPHENICOL DETECTION IN FOOD SAMPLES

Year 2025, Volume: 26 Issue: 4, 486 - 500, 25.12.2025

Nursaya Tokbayeva Ali Özcan

https://doi.org/10.18038/estubtda.1770428

Abstract

This study presents the development of a voltammetric sensing method utilizing a glassy carbon electrode modified with electrochemically exfoliated graphene oxide (EEGO) for detecting chloramphenicol (CAP), a broad-spectrum antibiotic of concern for food safety. EEGO was synthesized through an electrochemical exfoliation process using 0.25 M LiClO4 as the electrolyte and characterized using cyclic voltammetry, scanning electron microscopy, electrochemical impedance spectroscopy, and X-ray diffraction, confirming its favorable structural and electrochemical properties. The EEGO-modified electrode exhibited superior electrochemical performance compared to bare glassy carbon electrodes, offering a broader linear range (1.0–62.5 μM) and a lower detection limit (0.067 μM) for CAP. The enhanced performance of the EEGO-modified electrode can be attributed to the high surface area, excellent electrical conductivity, and abundant oxygen-containing functional groups of EEGO, which facilitate electron transfer and promote strong analyte adsorption. The proposed sensor demonstrated excellent selectivity and stability, maintaining its performance even in the presence of common interfering substances in food matrices. The developed method was successfully applied to determine CAP in milk and honey samples, with recovery values between 84.88% and 109.48%, demonstrating its potential for practical applications in food safety monitoring. The developed voltammetric method is characterized by its simplicity and high sensitivity, eliminating the need for complex sample pretreatment. This method effectively identifies CAP in food matrices, thereby contributing to the development of practical analytical tools for monitoring food safety.

Keywords

Chloramphenicol , Electrochemically Exfoliated Graphene Oxide , Modified Electrode , Voltammetry

Supporting Institution

Eskişehir Technical University

Project Number

24LÖT240

Thanks

We extend our profound gratitude to the Eskişehir Technical University Scientific Research Projects Commission for their financial support (Project No: 24LÖT240).

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