PHENOLIC COMPOUNDS DETERMINATION AND ANTIOXIDANT ACTIVITY OF TEUCRIUM CAVERNARUM
Öz
ABSTRACT
The current study was aimed to determine phenolic compounds profile and antioxidant activity of 70% MeOH extract of Teucrium cavernarum P.H. Davis which is an endemic plant of Turkey.
According to LC-MS/MS analysis of the extract, forsythoside A, forsythoside B, luteolin-7-gucoside, cirsimaritin and cirsiliol were determined as the major compounds. The total phenolic and radical scavenging activities of the extract were also determined. According to antioxidant activity results, IC50 value of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging effect was determined as 0.12 ±0.01 mg/mL. 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activity was also determined as 1.2 mM trolox equivalent (TEAC).
Anahtar Kelimeler
Kaynakça
- [1] Baytop T. Türkiyede Bitkiler ile Tedavi Geçmişte ve Bugün. 2nd ed. İstanbul, Turkey: İstanbul Üniversitesi Publication, 1999.
- [2] Nematollahi-Mahani SN, Rezazadeh-Kermani M, Mehrabani M,Nakhaee N. Cytotoxic Effects of Teucrium polium. on Some Established Cell Lines. Pharm Biol 2007; 45: 295-298.
- [3] Ozgen U, Mavi A, Terzi Z, Yιldιrιm A, Coşkun M, Houghton PJ. Antioxidant Properties of Some Medicinal Lamiaceae Labiatae Species. Pharm Biol 2006; 44: 107-112,
- [4] Sıngleton V.L, Orthofer R, Lamuela-Ravento´S RM. Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent, In: Packer L, editor. Methods in Enzymology. San Diego, USA: Academic Press 1999. Pp. 152-178
- [5] Papandreou MA, Kanakis CD, Polissiou MG, Efthimiopoulos S, Cordopatis P, Margarity M, Lamari FN. Inhibitory activity on amyloid-β aggregation and antioxidant properties of Crocus sativus stigmas extract and its crocin constituents. J. Agric. Food Chem 2006; 54: 8762-8768.
- [6] Kumarasamy Y, Byres M, Cox PJ, Jaspars M, Nahar L, Sarker SD. Screening seeds of some Scottish plants for free radical scavenging activity. Phytother Res 2007; 21: 615-621.
- [7] Clifford MN, Johnston KL, Knight S, Kuhnert N.. Hierarchical scheme for LC-MS Identification of chlorogenic acids. J. Agric. Food Chem 2003; 51: 2900-2911.
- [8] Mitreski I, Stanoeva JP, Stefova M, Stefkov G, Kulevanova S. 2014. Polyphenols in Representative Teucrium Species in the Flora of R. Macedonia: LC/DAD/ESI-MSn Profile and Content. Nat Prod Commun 2014; 9: 175-180
- [9] Parejo I, Jauregui O, Sanchez-Rabaneda F, Viladomat F, Bastida J, Codina C. (2004) Separation and characterization of phenolic compounds in fennel (Foeniculum vulgare) using liquid chromatography-negative electrospray ionization tandem mass spectrometry. Journal of Agricultural and Food Chemistry, 52, 3679-3687.
- [10]. Sánchez-Rabaneda F, Jáuregui O, Lamuela-Raventós RM, Bastida J, Viladomat F, Codina C. Identification of phenolic compounds in artichoke waste by high-performance liquid chromatography–tandem mass spectrometry. J. Chromatogr. A 2003; 1008: 57-72.