PHENOLIC COMPOUNDS DETERMINATION AND ANTIOXIDANT ACTIVITY OF TEUCRIUM CAVERNARUM

Year 2019, , 229 - 237, 31.07.2019

Fatih Göğer , Ayla Kaya Muhittin Dinç , Süleyman Doğu Doğu

https://doi.org/10.18036/estubtdc.599094

Abstract

ABSTRACT

The current study was aimed to
determine phenolic compounds profile and antioxidant activity of 70% MeOH
extract of Teucrium cavernarum  P.H. Davis which is an endemic plant of
Turkey.

 

According to LC-MS/MS analysis
of the extract, forsythoside A, forsythoside B, luteolin-7-gucoside,
cirsimaritin and cirsiliol were determined as the major compounds. The total
phenolic and radical scavenging activities of the extract were also determined.
According to antioxidant activity results, IC₅₀ value of
1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging effect was determined
as 0.12 ±0.01 mg/mL. 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)
(ABTS) radical scavenging activity was also determined as 1.2 mM trolox
equivalent (TEAC).

Keywords

Teucrium cavernarum, Antioxidant activity, LC-MS/MS, Phenolic

References

• [1] Baytop T. Türkiyede Bitkiler ile Tedavi Geçmişte ve Bugün. 2nd ed. İstanbul, Turkey: İstanbul Üniversitesi Publication, 1999.
• [2] Nematollahi-Mahani SN, Rezazadeh-Kermani M, Mehrabani M,Nakhaee N. Cytotoxic Effects of Teucrium polium. on Some Established Cell Lines. Pharm Biol 2007; 45: 295-298.
• [3] Ozgen U, Mavi A, Terzi Z, Yιldιrιm A, Coşkun M, Houghton PJ. Antioxidant Properties of Some Medicinal Lamiaceae Labiatae Species. Pharm Biol 2006; 44: 107-112,
• [4] Sıngleton V.L, Orthofer R, Lamuela-Ravento´S RM. Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent, In: Packer L, editor. Methods in Enzymology. San Diego, USA: Academic Press 1999. Pp. 152-178
• [5] Papandreou MA, Kanakis CD, Polissiou MG, Efthimiopoulos S, Cordopatis P, Margarity M, Lamari FN. Inhibitory activity on amyloid-β aggregation and antioxidant properties of Crocus sativus stigmas extract and its crocin constituents. J. Agric. Food Chem 2006; 54: 8762-8768.
• [6] Kumarasamy Y, Byres M, Cox PJ, Jaspars M, Nahar L, Sarker SD. Screening seeds of some Scottish plants for free radical scavenging activity. Phytother Res 2007; 21: 615-621.
• [7] Clifford MN, Johnston KL, Knight S, Kuhnert N.. Hierarchical scheme for LC-MS Identification of chlorogenic acids. J. Agric. Food Chem 2003; 51: 2900-2911.
• [8] Mitreski I, Stanoeva JP, Stefova M, Stefkov G, Kulevanova S. 2014. Polyphenols in Representative Teucrium Species in the Flora of R. Macedonia: LC/DAD/ESI-MSn Profile and Content. Nat Prod Commun 2014; 9: 175-180
• [9] Parejo I, Jauregui O, Sanchez-Rabaneda F, Viladomat F, Bastida J, Codina C. (2004) Separation and characterization of phenolic compounds in fennel (Foeniculum vulgare) using liquid chromatography-negative electrospray ionization tandem mass spectrometry. Journal of Agricultural and Food Chemistry, 52, 3679-3687.
• [10]. Sánchez-Rabaneda F, Jáuregui O, Lamuela-Raventós RM, Bastida J, Viladomat F, Codina C. Identification of phenolic compounds in artichoke waste by high-performance liquid chromatography–tandem mass spectrometry. J. Chromatogr. A 2003; 1008: 57-72.
• [11] Tepe B, Degerli S, Arslan S, Malatyali E, Sarikurkcu C. Determination of chemical profile, antioxidant, DNA damage protection and antiamoebic activities of Teucrium polium and Stachys iberica. Fitoterapia 2011; 82: 237-246.
• [12] Ozkan G, Kuleasan H, Celik S, Gokturk RS, Unal, O,. Screening of Turkish endemic Teucrium montbretii subsp pamphylicum extracts for antioxidant and antibacterial activities, Food Control 2007; 18: 509-512.
• [13] Kaya A, Demirci B, Dinc M, Dogu S, Baser KHC, 2013. Compositions of the Essential Oils of Teucrium cavernarum and Teucrium paederotoides, Two Endemic Species from Turkey. J Essent Oil Bear Pl 2013;16, 588-594.