The morphological analysis of anaplastic thyroid cancer cell line

Yıl 2022, Cilt: 61 Sayı: 3, 460 - 464, 12.09.2022

Hatice Özışık Berrin Ozdil Merve Ozdemir Murat Sipahi Mehmet Erdoğan Şevki Çetinkalp Ahmet Gökhan Özgen Füsun Saygılı Gülgün Oktay Hüseyin Aktuğ

https://doi.org/10.19161/etd.1168177

Öz

Aim: Thyroid follicular cell derived cancers are classified into three groups such as papillary thyroid cancer (85%), follicular thyroid cancer (12%) and anaplastic (undifferentiated) thyroid cancer (ATC) (3%). ATCs have very rapid course, poor treatment outcomes and they are very aggressive. The aim of current study was to assess the analysis of the morphological differences of ATC cell line with the normal thyroid cell line (NTC).

Materials and Methods: NTH and ATC cells were examined with haematoxylin and eosin, the nucleus: cytoplasm (N:C) ratios were detected, and cell cycles were investigated. These cell lines were compared according to their N:C ratio and their abundance in cell cycle phases.

Results: The N:C ratio was higher in ATC than NTC. Both cell groups were mostly found in G0/G1 phase (68.4; 82.8) and have statistical difference in both G0/G1 and S phases.

Conclusion: The rapid course and the rarity of ATC are significant barriers for clinical trials. Cultured cell lines are very important to explore the behaviour in the biology of ATC cells (such as the cell cycle), to understand the course of the disease, and to find an effective target for treatment.

Anahtar Kelimeler

Anaplastic thyroid cancer, cell cycle, apoptosis

Anaplastik tiroid kanseri hücre hattının morfolojik analizi

Öz

Amaç: Tiroid foliküler hücre kaynaklı kanserler papiller tiroid kanseri (%85), foliküler tiroid kanseri (%12) ve anaplastik tiroid kanseri (ATK) (%3) olmak üzere üç kategoriye ayrılır. ATK'lerin çok hızlı seyri, kötü tedavi sonuçları vardır ve çok agresiftirler. Bu çalışmanın amacı, ATK hücre hattının normal tiroid hücre hattı (NTH) ile olan morfolojik farklılıklarının analizini değerlendirmektir.

Gereç ve Yöntem: NTH ve ATK hücrelerinde hematoksilen-eosin boyama ile inceleme yapıldı, nükleus sitoplazma (N:S) oranlarına bakıldı ve hücre döngüleri incelendi. Bu hücre hatları N:S oranlarına ve hücre döngüsü fazlarındaki bulunma oranlarına göre karşılaştırıldı.

Bulgular: ATK hızlı seyri ve nadir görülmesi nedeniyle tedavisi zor bir kanser türüdür. Kültürlenmiş hücre hatlarında çalışmak, ATK hücrelerinin biyolojisindeki davranışları keşfetmek (hücre siklusu gibi), hastalığın seyrini anlamak ve tedavi için etkili bir hedef bulmak açısından önem taşımaktadır.

Sonuç: N:S oranı ATK'de NTH'den daha yüksekti. Her iki hücre grubu da çoğunlukla G0/G1 fazında (68.4; 82.8) bulundu ve hem G0/G1 hem de S fazlarında istatistiksel farklılıklara sahipti.

Anahtar Kelimeler

Anaplastik tiroid kanseri, hücre döngüsü, apoptoz

Kaynakça

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