Hıyarda (Cucumis sativus L.) Tuzluluk Stresi Koşullarında Genomik Stabilite Üzerinde Aşılamanın Etkisi

Öz

Hıyar, yüksek miktarda yetiştiriciliği yapılan önemli bir sebze türüdür. Abiyotik stres faktörleri hıyarda önemli morfolojik, fizyolojik ve moleküler değişikliklere neden olabilmektedir. Güçlü anaçlar üzerine aşılama bu olumsuz etkiyi azaltabilir. Bu çalışma, hıyarda tuzluluk stresinin genotoksik potansiyelini değerlendirmek ve aşılamanın genotoksisite üzerine etkisini belirlemek amacıyla yapılmıştır. Aşısız ve aşılı bitkiler 100 mM NaCl ile muamele edilmiş ve 15 gün sonunda ISSR profilleri karşılaştırılmıştır. On dört ISSR primeri kullanılarak tüm gruplar arasında %51.9 polimorfizm tespit edilmiştir. Tuzluluk stresi GTS oranını % 47.2'ye düşürürken aşılama ile bu oranın artırılabileceği belirlenmiştir. GTS ve benzerlik indeksleri benzer sonuçlar vermiş ve ISSR tekniğinin genotoksisite belirlemede etkili olabileceği sonucuna varılmıştır. Anaçlar arasında da ölçümler arasında farklılıklar tespit edilmiştir. Bu çalışma ile tuzluluk stresinin hıyarda genomik stabilite değişikliğine neden olabileceği, bu parametrenin kullanılan anaç çeşidine bağlı olarak aşılama ile korunabileceği, ISSR tekniğinin genotoksisite belirlemede kullanılabileceği sonucuna varılmıştır. Bu çalışma, tuzluluk stresine hıyar tepkisinde aşılamanın altında yatan moleküler mekanizmaları araştırmak için bir temel sağlamaktadır.

Anahtar Kelimeler

• Cucumis sativus
• tuz stresi
• aşılama
• GTS

Effects of Grafting on Genomic Stability in Salinity Stress Conditions in Cucumber (Cucumis sativus L.)

Abstract

Cucumber is an important type of vegetable that is grown in high quantities. Abiotic stress factors can cause significant morphological, physiological and molecular changes in cucumber. Grafting on strong rootstocks can reduce this negative effect. This study was carried out to evaluate the genotoxic potential of salinity stress in cucumber and to determine the effect of grafting on genotoxicity. Non-grafted and grafted plants were treated with 100 mM NaCl and the ISSR profiles were compared after 15 days. Using 14 ISSR primers, 51.9% polymorphism was detected between all groups. While salinity stress decreased the GTS rate to 47.2%, it was determined that this rate could be increased (%72.4-79.5) with grafting. GTS and similarity indices gave similar results and it was concluded that the ISSR technique could be effective in determining genotoxicity. There were also differences in measurements between rootstocks. With this study, it was concluded that salinity stress may cause genomic template stability changes in cucumber, this parameter can be protected by grafting depending on the rootstock variety used, and the ISSR technique can be used to determine genotoxicity. This study provides a basis for investigating the molecular mechanisms underlying grafting in the cucumber response to salinity stress.

Keywords

• Cucumis sativus
• salt stress
• grafting
• GTS

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