Ocimum Basilicum L. Uçucu Yağının Antifungal Etkileri

Ayşe Usanmaz Bozhüyük; Şaban Kordali

Ocimum Basilicum L. Uçucu Yağının Antifungal Etkileri

Öz

Ocimum basilicum L. uçucu yağı 9 fitopatojenik fungusu oluşturan altı Fusarium türüne (Fusarium equiseti, Fusarium graminearum, Fusarium oxysporum, Fusarium moniliforme, Fusarium sambucinum, Fusarium semitectum) ve Rhizoctonia solani, Alternaria solani, Verticillium dahliae funguslarının misel büyüme ve %engelleme oranlarına (in vitro) uygulanarak antifungal etkisi test edilmiştir. Çalışma sonuçları, O. basilicum uçucu yağının 15 ve 20 μL petri^-1’lik konsantrasyonlarının F. sambucinum fungusunun misel gelişimini tamamen inhibe etttiğini ve antifungal etkisinin ticari fungusit ‘’Captan’’dan daha yüksek olduğunu göstermiştir. O. basilicum uçucu yağının farklı konsantrasyonlarının (5, 10, 15 ve 20 μL petri^-1) tüm test edilen patojenik fungusların gelişimini önemli ölçüde engellemiştir. Yağın uygulanan tüm konsantrasyonlarında test edilen bütün patojenik fungusların misel gelişimini 0 dan 31.2 mm kadar değişen oranlarda etkilediği ve yağın antifungal aktivitesinin olduğu belirlenmiştir. O. basilicum uçucu yağı 9 bitki patojeni fungusun büyümesini %23-100 arasında engellediği görülmüştür. Bu çalışma ile O. basilicum uçucu yağının fungusitler gibi kullanılma potansiyeline sahip olduğu belirlenmiştir.

Anahtar Kelimeler

Origanum basilicum L.,Uçucu yağ,Antifungal etki

References

Akgül, A., 1989. Volatile oil composition of sweet basil (Ocimum basilicum L.) cultivating in Turkey. Molecular Nutrition & Food Research 33 (1): 87-88.
Alvarez-Castellanos, P. P., Bishop, C.D., and Pascual-Villalobos, M.J., 2001. Antifungal activity of essential oil of flowerheads of garland chrysanthemum (Chrysanthemum coronarium) against agricultural pathogens. Phytochemistry 57: 99-102.
Assawah, S., 2002. Effect of some plant extracts and essential oils on Fusarium wilt of broad bean. African Journal of Mycology and Biotechnology 10(3):75-86.
Baldim, J. L., Silveira, J. G. F., Almeida, A. P., Carvalho, P. L. N., Rosa, W., Schripsema, J., and Luiz, J. H. H., 2018. The synergistic effects of volatile constituents of Ocimum basilicum against foodborne pathogens. Industrial Crops and Products 112: 821-829.
Bayrak, A., and Akgül, A., 1987. Composition of essential oils from Turkish Salvia species. Phytochemistry 26: 846-847.
Boyraz, N., and Özcan, M., 1997. Bitki patojeni funguslara bazı yerli baharat ekstrakt ve uçucu yağlarının antifungal etkileri. Gıda 22 (6): 457-462.
Bozhüyük, A. U., Kordali, Ş., and Bölük, G., Satureja hortensis L. Uçucu Yağının Antifungal Etkisi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 46(2):107-112.
Chang, X. P., G. Alderson, and C. J. Wright., 2009. Variation in the essential oils in different leaves of basil (Ocimum basilicum L.) at day time. The Open Horticulture Journal 2: 13-16.

Daferera, D.J., Ziogas, B.N., and Polissiou, M.G., 2003. The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp. and Clavibacter michiganensis subsp. michiganensis Crop Protection 22: 39-44.
Döken, M.T., Demirci, E., and Eken, C., 2009. Bitki Fungal Hastalıkları Ders Notu. Atatürk Üniversitesi Yayınları, Erzurum.
Dube, S., Upadyay, P.D., and Tripathi, S.C., 1989. Antifungal physicochemical and insect-repellent activity of the essential oil of Ocimum basilicum. Canadian Journal of Botany 67: 2085-2087.
Herman, A., Tambor, K., and Herman, A., 2016. Linalool affects the antimicrobial efficacy of essential oils. Current Microbiology. 72: 165–172.
İşcan, G., 2002. Umbelliferae familyasına ait bazı bitki uçucu yağlarının antimikrobiyal aktivitelerinin araştırılması, Yüksek lisans tezi, Anadolu Üniversitesi Fen Bilimleri Enstitüsü, Eskişehir.
Knobloch, K., Pauli, A., Iberl, B., Weigand, H., and Weis, V., 1989. Antimicrobial and antifungal properties of essential oil components. Journal of Essential Oil Research 1: 119-128.
Kordali, S., Kotan, R., and Cakir, A., 2007. Screening of In Vitro Antifungal Activities of 21 Oxygenated Monoterpenes as Plant Disease Control Agents. Allelopathy Journal 19 :2, 373-391.
Macias, F.A., Castellano, D., Oliva, R.M., Cross, P., and Torres, A. 1997. Potential use of allelopathic agents as natural agrochemicals. Brighton Crop Protection Conference Weeds 33-38.
Martinovic, T., Andjelkovic, U., Gajdosik, M.S., Resetar, D., and Josic, D., 2016. Foodborne pathogens and their toxins. Journal of Proteomics 147: 226–235.
Mhiri, R., Kchaou, M., Belhadj, S., El Feki, A., and Allouche, N., 2018. Characterization of aromatic compounds and biological activities of essential oils from Tunisian aromatic plants. Journal of Food Measurement and Characterization 12(2):839-847.
Omidbaigi, R., 2005. Production and processing of medicinal plants, Astan Godesa Razavei Publication 1: 346.
Paton, A., Harley, R., and Harley, M., 1999, Ocimum-an overview of relationships and classification. Ocimum Aromatic Plants-Industrial Profiles. Amsterdam: Harwood Academic.
Piras, A., Gonçalves, M. J., Alves, J., Falconieri, D., Porcedda, S., Maxia, A., and Salgueiro, L., 2018. Ocimum tenuiflorum L. and Ocimum basilicum L., two spices of Lamiaceae family with bioactive essential oils. Industrial Crops and Products 113: 89-97.
Rathee, P.S., Mishra, S.H., and Kaushal, R., 1982. Antimicrobial activity of essential oil, fixed oil and unsaponifiable matter of Nigella sativa Linn. Indian Journal of Pharmacological Science 44: 8-10.
Romagnoli, C., Bruni, R., Andreotti, E., Raı, M.K., Vicentini, C.B. and Mares, D., 2005. Chemical characterization and antifungal activity of essential oil of capitula from wild Indian Tagetes patula L., Protoplasma 225, 57-65.
Shahi, S.K., Patra, M., Shukla, A.C., and Dikshit, A., 2003. Use of essential oil as botanical-pesticide against post harvest spoilage in Malus pumilo fruits. Biocontrol, 48:223-232.
Sharma, N., and Tripathi, A. 2006. Fungitoxicity of the essential oil of Citrus sinensis on post-harvest pathogens, World Journal of Microbiology & Biotechnology 22:587-593.
Shelef, L.A., 1983. Antimicrobial effects of spices. Journal of Food Safety 6:29-44.
Sokovic, M., Tzakou, O., Pitarokili, D., and Couladıs, M., 2002. Antifungal activities of selected aromatic plants growing wild in Greece. Nahrung-Food 46: 317-320.
Soylu, E.M., Yiğitbaş, H., Tok, F.M., Soylu, S., Kurt, Ş., Baysal, Ö., and Kaya, A.D., 2005, Chemical composition and antifungal activity of the essential oil of Artemisia annua L. against foliar and soil-borne fungal pathogens, Zeitschriftfür Pflanzenkrankheiten und Pflanzenschhutz 112: 229-239.
Soylu, E.M., Soylu, S., and Kurt, S., 2006. Antimicrobial Activities of the Essential Oils of Various Plants Against Tomato Late Blight Disease Agent Phytophthora infestans. Mycopathologia 161: 119-128. Soylu, E. M., Kurt, Ş. and Soylu, S., 2010. In vitro and in vivo activities of the essential oils of various plants against tomato grey mould disease agent Botrytis cinerea, International Journal of Food Microbiology 143: 183-189.
Silva, V.A., Sousa, J.P., Pessôa, H.L.F., Freitas, A.F.R., Coutinho, H.D.M., Alves, L.B.N., and Lima, E.O., 2016. Ocimum basilicum: Antibacterial activity and association study with antibiotics against bacteria of clinical importance. Pharmaceutical Biology 54: 863–867.
Simon, J. E., Quinn, J., and Murray, R. G., 1990. Basil: a source of essential oils. Advances in new crops 484-489.
Traka, C. K., Petrakis, E. A., Kimbaris, A. C., Polissiou, M. G., and Perdikis, D. C., 2018. Effects of Ocimum basilicum and Ruta chalepensis hydrosols on Aphis gossypii and Tetranychus urticae. Journal of Applied Entomology 142(4): 413-420.
Ultee, A., Bennik, M.H.J., Moezelaar, R., 2002. The phenolic hydroxyl group of carvacrol is essential for action against the food-borne pathogen Bacillus cereus. Applied and Environmental Microbiology 68 (4): 1561-1568.
Walter, M., Jaspers, M.V., Eade, K., Frampton, C.M., and Stewart, A., 2001. Control of Botrytis cinerea in grape using thyme oil. Australasian Plant Pathology 30: 21-25.

Details

Primary Language

Turkish

Subjects

Agricultural Engineering

Journal Section

Research Article

Authors

Ayşe Usanmaz Bozhüyük ^*
0000-0003-2450-6850
Türkiye

Şaban Kordali
0000-0001-5669-5831
Türkiye

Publication Date

February 8, 2019

Submission Date

December 3, 2018

Acceptance Date

February 8, 2019

Published in Issue

Year 2019 Volume: 2 Number: 1

IZ

https://izlik.org/JA46TP86ZB

Cite

RIS / Bibtex

APA

Usanmaz Bozhüyük, A., & Kordali, Ş. (2019). Ocimum Basilicum L. Uçucu Yağının Antifungal Etkileri. Erciyes Tarım Ve Hayvan Bilimleri Dergisi, 2(1), 45-57. https://izlik.org/JA46TP86ZB