The Effects of Bio-Priming on Seed Germination and Seedling Growth of Italian Ryegrass (Lolium multiflorum Lam.)

Abstract

Seed bio-priming applications with plant growth promoting rhizobacteria (PGPR) have been widely used recently to improve germination and seedling growth. Therefore, the aim of this study was to investigate the effects of bio-priming with different bacterial strains on germination and seedling development of Italian ryegrass seeds. The sterilized seeds of the Elif variety (Lolium multiflorum Lam) were inoculated with nine different bacterial strains belonging to Bacillus species (108 cfu/mL bacterial suspension) for 15 min at 120 rpm and then dried at room temperature. The treated seeds were germinated in petri dishes with 25 seeds between 3 filter papers at 22 ±2 ˚C. The study was carried out in a completely randomized design with three replications. As a result of the study, no significant difference was obtained between the treatments in germination percentage and root length, but it was determined that SY2 and SY5 (Bacillus isolates) showed superior performance compared to the control in terms of shoot length and seedling fresh and dry weights.

Keywords

• Germination
• Lolium multiflorum Lam
• PGPR
• priming

Biyo-Primingin İtalyan Çimi (Lolium multiflorum Lam.) Tohum Çimlenmesi ve Fide Büyümesi Üzerine Etkileri

Öz

Bitki büyümesini teşvik eden rizobakteriler (PGPR) ile tohum biyo-priming uygulamaları, çimlenmeyi ve fide gelişimini iyileştirmek amacıyla son zamanlarda yaygın olarak kullanılmaktadır. Bu nedenle, bu çalışmanın amacı, farklı bakteri suşları ile biyo-priming uygulamalarının İtalyan çimi tohumlarının çimlenmesi ve fide gelişimi üzerindeki etkilerini araştırmaktır. Çalışmada Elif çeşidi (Lolium multiflorum Lam) İtalyan çimi tohumları kullanılmıştır ilk olarak sterilize edilmiş tohumlar Bacillus türlerine ait dokuz farklı bakteri suşu (108 cfu/mL bakteri süspansiyonu) ile 120 rpm'de 15 dakika süreyle inoküle edilmiş ve ardından oda sıcaklığında kurutulmuştur. Uygulama yapılan tohumlar petri kaplarında 3 filtre kağıdı arasında 25 tohum olacak şekilde 22 ±2 ˚C'de çimlendirilmiştir. Çalışma üç tekerrürlü olarak tesadüf parselleri deneme desenine göre yürütülmüştür. Çalışma sonucunda, çimlenme yüzdesi ve kök uzunluğu bakımından uygulamalar arasında önemli bir fark elde edilmemiş, ancak SY2 ve SY5'in (Bacillus izolatları) sürgün uzunluğu ve fide taze ve kuru ağırlıkları bakımından kontrole kıyasla üstün performans gösterdiği belirlenmiştir.

Anahtar Kelimeler

• Lolium multiflorum Lam
• çimlenme
• PGPR
• priming

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