Objectives:
In this study, we evaluated a rapid and simple protocol for direct
identification of microorganisms with Matrix-Assisted Laser
Desorption/Ionization Time of Flight, Mass Spectrometry (MALDI-TOF MS) after a
short incubation in solid medium (3 to 5h).
Methods: We have examined a total
of 1101 positive blood culture bottles from 782 patients.
Results: We obtained a correct
identification in 1037 (94.18%); 190 (98.44%) were Staphylococcus aureus, and 386 (98.44%) Enterobacteriaceae. Both are the most
frequent etiological agents of sepsis. A total of 1004 bottles were monomicrobial
(96.81%) and 33 (3.18%), polimicrobial. In the latter we identified at least one
species. Two hundred eighty (27.00%) organism isolated were considered skin
contaminant. Carbapenemase tests were performed with Blue-Carba test in 140
patinas from Gram negative bacilli, we have detected earlier 27 of 29 positive
(93.10%).
Conclusions: Mass spectrometry by MALDI-TOF MS is very useful
to quickly identify the microbial agent and Blue-Carba contribute to adapt the
antibiotic therapy to obtain a correct clinical management of the patient with
bacteremia.
MALDI-TOF MS rapid identification blood culture carbapenemase short incubation
We are grateful to Tamara Posse and Luis Aquino for their technical assistance.
Birincil Dil | İngilizce |
---|---|
Konular | Tıbbi Mikrobiyoloji |
Bölüm | Original Article |
Yazarlar | |
Yayımlanma Tarihi | 4 Mart 2020 |
Gönderilme Tarihi | 11 Ekim 2018 |
Kabul Tarihi | 26 Kasım 2019 |
Yayımlandığı Sayı | Yıl 2020 Cilt: 6 Sayı: 2 |