The Effects of Panax ginseng on Serum Oxidative Stress Following Bisphenol A Exposure

Abstract

Objective: Bisphenol A (BPA) is a toxic compound that causes oxidative stress by disrupting antioxidant enzymes and promoting tissue lipid peroxidation. This study aimed to examine the impacts of BPA on serum oxidative stress in rats and to detect the antioxidant feature of Panax ginseng (PxG) in reducing BPA-induced oxidative stress. Materials and Methods: Wistar Albino rats (250-300 g) were divided into control, control + PxG, BPA, and BPA + PxG groups. 50 mg/kg BPA and 100 mg/g PxG were given for six weeks. Serum total antioxidant and oxidant status, lipid peroxidation, and glutathione levels were determined. Results: BPA administration increased total oxidant status and lipid peroxidation, while PxG administration to the BPA group decreased these parameters. PxG also increased total antioxidant status and glutathione levels compared to the BPA group. Conclusion: BPA was seen to cause an increase in oxidative parameters and PxG administration to restore the oxidative stress that was generated after BPA exposure, suggesting that this may help to prevent the adverse effects caused by BPA exposure.

Keywords

• Bisphenol A
• Panax ginseng
• oxidative stress
• antioxidant effect

References

1. 1. Rubin BS. Bisphenol A: an endocrine disruptor with widespread exposure and multiple effects. J Steroid Biochem Mol Biol 2011; 127(1-2): 27-34. google scholar
2. 2. Gassman NR. Induction of oxidative stress by bisphenol A and its pleiotropic effects. Environ Mol Mutagen 2017; 58(2): 60-71. google scholar
3. 3. Xin F, Jiang L, Liu X, Geng C, Wang W, Zhong L, et al. Bisphenol A induces oxidative stress-associated DNA damage in INS-1 cells. Mutat Res Genet Toxicol Environ Mutagen 2014; 769: 29-33. google scholar
4. 4. Erler C, Novak J. Bisphenol a exposure: human risk and health policy. J Pediatr Nurs 2010; 25(5): 400-7. google scholar
5. 5. Meli R, Monnolo A, Annunziata C, Pirozzi C, Ferrante MC. Oxidative stress and BPA toxicity: an antioxidant approach for male and female reproductive dysfunction. Antioxidants 2020; 9(5): 405. google scholar
6. 6. Ishtiaq A, Bakhtiar A, Silas E, Saeed J, Ajmal S, Mushtaq I, et al. Pistacia integerrima alleviated Bisphenol A induced toxicity through Ubc13/p53 signalling. Mol Biol Rep 2020; 47: 6545-59. google scholar
7. 7. Abdou HM, Abd Elkader HAE, El-Gendy AH, Eweda SM. Neurotoxicity and neuroinflammatory effects of bisphenol A in male rats: The neuroprotective role of grape seed proanthocyanidins. Environ Sci Pollut Res Int 2022; 29(6): 9257-68. google scholar
8. 8. Johnson M, Pace RD. Sweet potato leaves: properties and synergistic interactions that promote health and prevent disease. Nutr Rev 2010; 68(10): 604-15. google scholar

9. 9. Zaid SS, Othman S, Kassim NM. Potential protective effect of Tualang honey on BPA-induced ovarian toxicity in prepubertal rat. BMC Complement Altern Med 2014; 14: 509. google scholar
10. 10. El Bana E, Kamal K, Shaheen N, Abowafa S. The protective role of sesame oil against bisphenol A-induced cardiotoxicity: A histological and immunohistochemical study. Kasr Al Ainy Med J 2019; 25(3): 87-98. google scholar
11. 11. Yun TK. Brief introduction of Panax ginseng C.A. Meyer. J Korean Med Sci 2001; 16 Suppl(Suppl): S3-5. google scholar
12. 12. Potenza MA, Montagnani M, Santacroce L, Charitos IA, Bottalico L. Ancient herbal therapy: A brief history of Panax ginseng. J Ginseng Res 2023; 47(3): 359-65. google scholar
13. 13. He B, Chen D, Zhang X, Yang R, Yang Y, Chen P, et al. Oxidative stress and ginsenosides: An update on the molecular mechanisms. Oxid Med Cell Longev 2022; 2022: 9299574. google scholar
14. 14. Liu H, Lu X, Hu Y, Fan X. Chemical constituents of Panax ginseng and Panax notoginseng explain why they differ in therapeutic efficacy. Pharmacol Res 2020; 161: 105263. google scholar
15. 15. Coleman CI, Hebert JH, Reddy P. The effects of Panax ginseng on quality of life. J Clin Pharm Ther 2003; 28(1): 5-15. google scholar
16. 16. Leung KW, Wong AS. Ginseng and male reproductive function. Spermatogenesis 2013; 3(3): e26391. google scholar
17. 17. Song H, Lee YY, Park J, Lee Y. Korean Red Ginseng suppresses bisphenol A-induced expression of cyclooxygenase-2 and cellular migration of A549 human lung cancer cell through inhibition of reactive oxygen species. J Ginseng Res 2021; 45(1): 119-25. google scholar
18. 18. Ok S, Kang JS, Kim KM. Cultivated wild ginseng extracts upregulate the anti-apoptosis systems in cells and mice induced by bisphenol A. Mol Cell Toxicol 2017; 13(1): 73-82. google scholar
19. 19. Lee J, Park J, Lee YY, Lee Y. Comparative transcriptome analysis of the protective effects of Korean Red Ginseng against the influence of bisphenol A in the liver and uterus of ovariectomized mice. J Ginseng Res 2020; 44(3): 519-26. google scholar
20. 20. Yagi K. Simple assay for the level of total lipid peroxides in serum or plasma. Methods Mol Biol 1998; 108: 101-6. google scholar
21. 21. Beutler E. Red cell metabolism: a manual of biochemical methods. 3rded.; Grune&Stratton; Orlando; FL, 1984. google scholar
22. 22. Ayala A, Munoz MF, Argüelles S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev 2014; 2014: 360438. google scholar
23. 23. Zhang H, Yang R, Shi W, Zhou X, Sun S. The association between bisphenol A exposure and oxidative damage in rats/mice: A systematic review and meta-analysis. Environ Pollut 2022; 292(Pt B): 118444. google scholar
24. 24. Ozaydin T, Oznurlu Y, Sur E, Celik I, Uluisik D, Dayan MO. Effects of bisphenol A on antioxidant system and lipid profile in rats. Biotech Histochem 2018; 93(4): 231-8. google scholar
25. 25. Ge LC, Chen ZJ, Liu H, Zhang KS, Su Q, Ma XY, et al. Signaling related with biphasic effects of bisphenol A (BPA) on Sertoli cell proliferation: a comparative proteomic analysis. Biochim Biophys Acta 2014; 1840(9): 2663-73. google scholar
26. 26. Tiwari D, Kamble J, Chilgunde S, Patil P, Maru G, Kawle D, et al. Clastogenic and mutagenic effects of bisphenol A: an endocrine disruptor. Mutat Res 2012; 743(1-2): 83-90. google scholar
27. 27. Moghaddam HS, Samarghandian S, Farkhondeh T. Effect of bisphenol A on blood glucose, lipid profile and oxidative stress indices in adult male mice Toxicol Mech Methods 2015; 25(7): 507-13 google scholar
28. 28. Kim SH, Park KS, Chang MJ, Sung JH Effects of Panax ginseng extract on exercise-induced oxidative stress J Sports Med Phys Fitness 2005; 45(2): 178-82 google scholar
29. 29. Ramesh T, Kim SW, Sung JH, Hwang SY, Sohn SH, Yoo SK, et al Effect of fermented Panax ginseng extract (GINST) on oxidative stress and antioxidant activities in major organs of aged rats Exp Gerontol 2012; 47(1): 77-84 google scholar
30. 30. Chung TH, Kim JH, Seol SY, Kim YJ, Lee YJ. The Effects of Korean red ginseng on biological aging and antioxidant capacity in postmenopausal women: a double-blind randomized controlled study. Nutrients 2021; 13(9): 3090. google scholar