DNA Binding, Nuclease/Photonuclease, and Phototoxicty Properties of Water Soluble Silicon (IV) Phthalocyanine
Abstract
Photodynamic therapy (PDT) is known as a method in which photosensitizers produce reactive oxygen species in the presence of light and oxygen, leading to cell death. In this paper, DNA interaction properties of bis[4-({8)-[3-(trimethylamino)phenoxy]octyl}oxy)] substituted silicon (IV) phthalocyanine (GsB-SiPc) were examined using a UV-Vis spectrophometer and agarose gel electrophoresis techniques. Afterwards, cytotoxic/phototoxic effects of GsB-SiPc were examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays on A549 cells. The results showed that GsB-SiPc bound to ct-DNA via a groove binding mode. In nuclease/photonuclease experiments, GsB-SiPc had low nuclease activity in the dark but it showed high photonuclease activity in the presence of light, depending on compound concentration and light dose. In addition, GsB-SiPc demonstrated remarkable phototoxicity toward human lung adenocarcinoma (A549) cell line at 50 and 100 µM in the presence of light. The in vitro data revealed the potential of GsB-SiPc as a photodynamic therapy agent for the treatment of lung cancer. These findings need to be supported by further studies.
Keywords
DNA Binding, DNA Nuclease, Lung Cancer, Photodynamic Therapy, Silicon (IV) Phthalocyanine
Ethical Statement
Since we did not use human/animal or human/animal data in this study, our study does not require ethics committee approval.
References
- Bray F, Laversanne M, Sung H, et al. Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2024;74(3):229-263. doi:10.3322/caac.21834
- Debela DT, Muzazu SG, Heraro KD, et al. New approaches and procedures for cancer treatment: Current perspectives. SAGE Open Med. 2021;9:20503121211034366. doi:10.1177/20503121211034366
- Nguyen VN, Pham HL, Nguyen XT. Recent progress in organic carbon dot-based photosensitizers for photodynamic cancer therapy. Dyes and Pigments. 2024;230:112359. doi: 10.1016/j.dyepig.2024.112359
- Baskaran R, Lee J, Yang SG. Clinical development of photodynamic agents and therapeutic applications. Biomater Res. 2018;22:25. doi:10.1186/s40824-018-0140-z
- Li X, Lovell JF, Yoon J, Chen X. Clinical development and potential of photothermal and photodynamic therapies for cancer. Nat Rev Clin Oncol. 2020;17(11):657-674. doi:10.1038/s41571-020-0410-2
- Karbasi M, Varzandeh M, Karbasi M, Mobarakeh AI, Falahati M, Hamblin MR. Photodynamic therapy based on metal-organic framework in cancer treatment: A comprehensive review of integration strategies for synergistic combination therapies. Nano-Structures & Nano-Objects. 2024;40:101315. doi:10.1016/j.nanoso.2024.101315
- Kim TE, Chang JE. Recent Studies in Photodynamic Therapy for Cancer Treatment: From Basic Research to Clinical Trials. Pharmaceutics. 2023;15(9):2257. doi:10.3390/pharmaceutics15092257
- Kwiatkowski S, Knap B, Przystupski D, et al. Photodynamic therapy - mechanisms, photosensitizers and combinations. Biomed Pharmacother. 2018;106:1098-1107. doi:10.1016/j.biopha.2018.07.049
- Oluwajembola AM, Cleanclay WD, Onyia AF, et al. Photosensitizers in photodynamic therapy: An advancement in cancer treatment. Results in Chemistry. 2024;10:101715. doi:10.1016/j.rechem.2024.101715
- Kessel D. Death pathways associated with photodynamic therapy. Photochemistry and Photobiology. 2021;97(5):1101-1103. doi:10.1111/php.13436