Determination of antioxidant, antimicrobial activities, total phenolic and flavonoid contents of Allium rumelicum, Jurinea kilaea and Peucedanum obtusifolium

Abstract

A microwave-assisted extraction (MAE) process for polyphenols from Allium rumelicum Kocyigit & Ozhatay, Jurinea kilaea Azn. and Peucedanum obtusifolium Sibth. & Sm. was used. This research examined the methanolic extracts made from these three species’ antioxidant, antimicrobial, total phenolic, and flavonoid contents. By using the 2,2-diphenyl-1-picryl-hydrazyl-hydrate free radical method (DPPH), ABTS/Persulfate, and Cupric reducing antioxidant capacity (CUPRAC) methods, the total antioxidant activities and capacities were examined. Additionally, the Folin-Ciocalteu and AlCl3/KAc techniques were used to calculate the total phenolic and flavonoid contents. To ascertain the antibacterial capabilities of plants, the disc diffusion method was applied. The J. kilaea showed the greatest total antioxidant capacity/activity levels when measured using the CUPRAC and ABTS/Persulfate techniques. A. rumelicum was found to have the highest quercetin concentration, while P. obtusifolium had the lowest. In J. kilaea, the gallic acid concentration was highest. The highest antimicrobial activity values were obtained in P. obtusifolium.

Keywords

• Antimicrobial activity
• antioxidant activity
• flavonoid
• total phenolic
• spectrophotometric method

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