PREPARATION OF FILM ELECTRODE USING DOPANT FOR CONSTRUCTION OF BIOSENSORS SENSITIVE TO HYDROGEN PEROXIDE

Abstract

In this study, a new film electrode for determination of hydrogen peroxide was developed. Polypyrrole-poly (sodium-4-styrenesulphonate) film have been prepared on the platinum electrode by the electropolymerization of pyrrole was carried out in the presence of poly(sodium-4-styrenesulphonate). Optimum conditions of polypyrrole-poly(sodium-4-styrenesulphonate) film was founded. Oxidation of the hydrogen peroxide was examined at a different potential. Therefore, four different potentials (0.4, 0.5, 0.6, and 0.7 V) were determined. Also, concentration of pyrrole and poly(sodium-4-styrenesulphonate) were examined. The effect of the number of cycles in their sensitivity to hydrogen peroxide is investigated. Morphology of electrodes was qualified by means of SEM. Free enzyme work was performed using the prepared film electrode and glucose oxidase. Linear increases were observed in the amperometric response currents of the electrode versus increasing glucose concentrations. It was shown that this film can be used for hydrogen peroxide producing enzymes. 

Keywords

• film electrode,hydrogen peroxide,polypyrrole,poly(sodium-4-styrenesulphonate)

References

1. 1. Ahmed, W., Sidhu, J.P.S., Toze S., “Speciation and frequency of virulence genes of Enterococcus spp. isolated from rainwater tank samples in southeast Queensland, Australia.” Environmental Science and Technology, 46(12):6843-6850, (2012).
2. 2. Wang, Z., Liu, F., Teng, X., Zhao, C., Lu C. “Detection of hydrogen peroxide in rainwater based on Mg-Al-carbonate layered double hydroxides-catalyzed luminol chemiluminescence”, Analyst, 136:4986-4990, (2011).
3. 3. Campelo, G., Souza, S.D., Bezerra, P.A., Paula, A., Paim, S., Marques, D., Paim, A.P.S., Lavorante, A.F. “A multicommuted flow system for fast screening/sequential spectrophotometric determination of dichromate, salicylic acid, hydrogen peroxide and starch in milk samples”,Food Control, 46:127-135, (2014).
4. 4. Hu, A., Liu, Y., Deng, H., Hong, G., Liu, A. “Fluorescent hydrogen peroxide sensor based on cupric oxide nanoparticles and its application for glucose and L-lactate detection”, Biosens. Bioelectron., 61: 374-378,2014.
5. 5. Lu, S., Song, J., Campbell-Palmer, L. “A modified chemiluminescence method for hydrogen peroxide determination in apple fruit tissues”, Sci. Hortic., 120: 336-341, (2009).
6. 6. Nakamura, H., Mogi, Y., Akimoto, T., Naemura, K., Kato, T., Yano, K., Karube, I. “An enzyme-chromogenic surface plasmon resonance biosensor probe for hydrogen peroxide determination using a modified Trinder’s reagent”, Biosens. Bioelectron. 24: 455-460, (2008).
7. 7. Rodrigues, S.S.M., Ribeiro, D.S.M., Molina-garcia, L., Medina, A.R., Prior, J.A.V., Santos, J.L.M. 2014. “Fluorescence enhancement of CdTe MPA-capped quantum dots by glutathione for hydrogen peroxide determination”, Talanta, 122:157-165, (2014).
8. 8. Baghayeria, M., Veisib, H., “Fabrication of a facile electrochemical biosensor for hydrogen peroxide using efficient catalysis of hemoglobin on the porous Pd@Fe3O4-MWCNT nanocomposite”, Biosensors and Bioelectronics, 74:190-198, (2015).

9. 9. Choi, M.M.F., Yiu, T.P. “Immobilization of beef liver catalase on eggshell membrane for fabrication of hydrogen peroxide biosensor”, Enzym. Microb. Technol., 34:41-47, (2004).
10. 10. Pei, J., Li, X., “Xanthine and hypoxanthine sensors based on xanthine oxidase immobilize on a CuPtCl6 chemically modified electrode and liquid chromatography electrochemical detection”, Anal. Chim. Acta, 414: 205-213, (2000).