KURŞUNUN LENS CULINARIS MEDİK. TOHUMLARININ ÇİMLENMESİ, KÖK GELİŞİMİ VE KÖK UCU HÜCRELERİ ÜZERİNDEKİ MİTOTİK ETKİLERİ

Abstract

Bu çalışmada, önemli çevre kirleticilerinden biri olan kurşun (PbCl2)’un mercimek (Lens culinaris Medik) tohumlarının çimlenmesi, kök büyümesi ve kök ucu hücrelerinin mitoz bölünmeleri üzerine etkileri araştırılmıştır. Denemelerde Pb+2’un farklı konsantrasyonları (0.125, 0.250, 0.500 ve 1.000 mM) kullanılmıştır. Düşük Pb++ konsantrasyonları ile muamele edilen tohumların çimlenmesinde kontrole göre belirgin bir farkın olmadığı, ancak yüksek konsantrasyonlarda çimlenmenin azaldığı gözlenmiştir. Ayrıca uygulanan tüm konsantrasyonlarda, kök büyümesi kontrole göre engellenmiştir. Kurşunun konsantrasyon artışına paralel olarak, hücre bölünmesinin azaldığı, c-mitoz, kalgın kromozom, multipolar anafaz ve köprü gibi çeşitli mitotik anormalliklerin arttığı tespit edilmiştir

Keywords

• Kurşun
• Lens culinaris
• çimlenme
• mitoz
• ağır metal

THE EFFECTS OF THE LEAD ON THE SEED GERMINATION, ROOT GROWTH, AND ROOT TIP CELL MITOTIC DIVISONS OF LENS CULINARIS MEDIK

Abstract

In this study, the effects of lead (PbCl2) one of the significant environmental pollutant, on seed germination, root growth and mitotic divisions of the root tip cells of lentil (Lens culinaris Medik.) were investigated. Different concentrations (0.125, 0.250, 0.500 and 1.000 mM Pb+2) of lead were applied. It was observed that there are no significant differences in the germination of seeds that exposed to low lead concentrations. On the other hand, at higher concentrations of lead inhibited germination. In addition, root growth was inhibited according to the control group at all concentrations. In parallel to the increase of the lead concentrations cell division was decreased, several mitotic anomalies such as cmitosis, lagging chromosomes, multipolar anaphases and Chromosome bridges were increased.

Keywords

• Lens culinaris
• germination
• mitosis
• heavy metals
• Lead

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