Conference Paper

Evaluation of the Protein Secondary Structures Using Fourier Transform Infrared Spectroscopy

Volume: 27 Number: 1 March 28, 2014
EN

Evaluation of the Protein Secondary Structures Using Fourier Transform Infrared Spectroscopy

Abstract

Fourier transform infrared (FTIR) spectroscopy is an attractive tool for proteomics research as it can be used to rapidly characterize protein secondary structure in aqueous solution. Fourier transform infrared spectrometry is well known as a powerful tool for determination of secondary structures of proteins. This paper presents the most recent experimental results obtained in our laboratory for this kind of analysis. A PeakFit operation was performed and some observations were made.

Keywords

References

  1. [1]. Chou and Fasman, G. D., “Prediction of the secondary structure of proteins from their amino acid sequence”, Adv. Enzymol, 47: 45-148 (1978).
  2. [2]. Fasman, G.D., “Protein conformational prediction”, Trends Biochem. Sci., 14: 295-299 (1989).
  3. [3]. Braun, W., “Distance geometry and related methods for protein structure determination from NMR data”, Quarterly Rev. Biophys., 19: 115- 157 (1987)
  4. [4]. Wlodawer. A., Bott, R. and Sjoh, L., “The refined crystal structure of ribonuclease A at 2.0 A resolution”, J. Biol. Chem., 2S7: 1325-1332 (1982)
  5. [5]. Pauling, L. and Corey, R. B., “Configurations of Polypeptide Chains With Favored Orientations Around Single Bonds: Two New Pleated Sheets”, Proc. Natl. Acad. Sci. USA, 37: 729-740 (1951).
  6. [6]. Arrondo, J. L. R.; Muga, A.; Castresana, J., and God. F. M., “Quantitative studies of the structure of proteins in solution by fourier-transform infrared spectroscopy”, Prog. Biophys. Mol. Biol., 59: 23-56 (1993).
  7. [7]. Susi, H. and Byler, D. M., “Resolution-enhanced Fourier transform infrared spectroscopy of enzymes”, Methods Enzymol., 130: 290-311 (1986).
  8. [8]. Byler, D. M. and Susi, H., “Examination of the secondary structure of proteins by deconvolved FTIR spectra”, Biopolymers, 25: 469-487 (1986).

Details

Primary Language

English

Subjects

Engineering

Journal Section

Conference Paper

Authors

Andrei Bunacıu This is me

Serban Fleschın This is me

Publication Date

March 28, 2014

Submission Date

March 12, 2013

Acceptance Date

-

Published in Issue

Year 2014 Volume: 27 Number: 1

APA
Aboul-eneın, Y., Bunacıu, A., & Fleschın, S. (2014). Evaluation of the Protein Secondary Structures Using Fourier Transform Infrared Spectroscopy. Gazi University Journal of Science, 27(1), 637-644. https://izlik.org/JA79LR54XE
AMA
1.Aboul-eneın Y, Bunacıu A, Fleschın S. Evaluation of the Protein Secondary Structures Using Fourier Transform Infrared Spectroscopy. Gazi University Journal of Science. 2014;27(1):637-644. https://izlik.org/JA79LR54XE
Chicago
Aboul-eneın, Y, Andrei Bunacıu, and Serban Fleschın. 2014. “Evaluation of the Protein Secondary Structures Using Fourier Transform Infrared Spectroscopy”. Gazi University Journal of Science 27 (1): 637-44. https://izlik.org/JA79LR54XE.
EndNote
Aboul-eneın Y, Bunacıu A, Fleschın S (March 1, 2014) Evaluation of the Protein Secondary Structures Using Fourier Transform Infrared Spectroscopy. Gazi University Journal of Science 27 1 637–644.
IEEE
[1]Y. Aboul-eneın, A. Bunacıu, and S. Fleschın, “Evaluation of the Protein Secondary Structures Using Fourier Transform Infrared Spectroscopy”, Gazi University Journal of Science, vol. 27, no. 1, pp. 637–644, Mar. 2014, [Online]. Available: https://izlik.org/JA79LR54XE
ISNAD
Aboul-eneın, Y - Bunacıu, Andrei - Fleschın, Serban. “Evaluation of the Protein Secondary Structures Using Fourier Transform Infrared Spectroscopy”. Gazi University Journal of Science 27/1 (March 1, 2014): 637-644. https://izlik.org/JA79LR54XE.
JAMA
1.Aboul-eneın Y, Bunacıu A, Fleschın S. Evaluation of the Protein Secondary Structures Using Fourier Transform Infrared Spectroscopy. Gazi University Journal of Science. 2014;27:637–644.
MLA
Aboul-eneın, Y, et al. “Evaluation of the Protein Secondary Structures Using Fourier Transform Infrared Spectroscopy”. Gazi University Journal of Science, vol. 27, no. 1, Mar. 2014, pp. 637-44, https://izlik.org/JA79LR54XE.
Vancouver
1.Y Aboul-eneın, Andrei Bunacıu, Serban Fleschın. Evaluation of the Protein Secondary Structures Using Fourier Transform Infrared Spectroscopy. Gazi University Journal of Science [Internet]. 2014 Mar. 1;27(1):637-44. Available from: https://izlik.org/JA79LR54XE