Effects of Sodium Selenite Supplementation on Lead Nitrate-induced Oxidative Stress in Lung Tissue of Diabetic and Non-diabetic Rats

Abstract

In this study, diabetic and non-diabetic male rats were given to sodium selenite, lead nitrate and sodium selenite plus lead nitrate through gavage. At the end of the 4^th week, lipid peroxidation and antioxidant enzyme activities was investigated compared to control group. No significant differences were observed between control and sodium selenite treated groups. By the end of the fourth week, lead nitrate led to increase the levels of MDA, and decrease in antioxidant activities compared with the control group in diabetic and non-diabetic rats. As a result, sodium selenite significantly reduces lead nitrate induced testicular toxicity both groups.

Keywords

• Lead nitrate
• sodium selenite
• oxidative stress
• diabetes
• lung

References

1. Shabani, A., Rabbani, A., “Lead nitrate induced apoptosis in alveolar macrophages from rat lung”, Toxicology 149: 109-114, (2000).
2. Khalaf, A.A., Moselhy, W.A., Abdel-Hamed, M.I., “The protective effect of green tea extract on lead induced oxidative and DNA damage on rat brain”. Neorotoxicity 33: 280-289, (2012).
3. Liu, M., Xu, Y., Chen, Y., Li, J., Zhao, F., Zheng, G., Jing, J., Ke, T., Chen, J., Luo, W., “The effect of sodium selenite on lead induced cognitive dysfunction”, Neurotoxicology 36: 82-88 (2013).
4. Moreira, E.G., Rosa, G.J.M., Barros, S.B.M., Vassilieff, V.S., Vassilieff I., “Antioxidant defense in rat brain regions after developmental lead exposure”, Toxicology 169: 145–151, (2001).
5. Ercal, N., Neal, R., Treeratphan, P., Lutz, P.M., Hammond, T.C., Dennery, P.A., Spitz, D.R., “A role for immunoglobulin levels in lead-exposed Fisher 344 rats”, Arch. Environ. Contam. Toxicol. 39: 251–256, (2000). suppressing serum
6. Shalan, M.G., Mostafa, M.S., Hassouna, M.M., El,Nabi, S.E.H., El-Rafeie, A., “Amelioration of lead toxicity on rat liver with Vitamin C and silymarin supplements”, Toxicology 206: 1–15, (2005).
7. Mousa, H.M., Al-Qarawi, A.A., Ali, B.H., Abdel Rahman, H.A., ElMougy, S.A., “Effect of lead exposure on the erythrocytic antioxidant levels in goats”, J. Vet. Med. A 49: 531–534 (2002).
8. Berrahal, A.A., Nehdi, A., Hajjaji, N., Gharbi, N., El- Fazaa, S., “Antioxidant enzymes activities and bilirubin level in adult rat treated with lead”, C.R. Biologies 330, 581-588, (2007).

9. El-Neweshy, M.S., El-Sayed, Y.S., “Influence of
10. supplementation vitamin C on lead-induced histopathological Experimental and Toxicologic Pathology 63: 221- 227 (2011). in male rats”,
11. Jihen, E., Imed, M., Fatima, H., Abdelhamid, K., “Protective effects of selenium (Se) and Zinc (Zn) on cadmium (Cd) toxicity in the liver of the rat: Effects on the oxidative stress”, Ecotoxicology and Environmental Safety 72: 1559-1564 (2009).
12. Kalender, S., Uzun, F.G., Demir, F., Uzunhisarcikli, M., Aslanturk., A., “Mercuric chloride-induced testicular toxicity in rats and the protective role of sodium selenite and vitamin E”. Food Chem Toxicol, 55: 456-462 (2013).
13. Prabhakar, P.K., Kumar, A., Doble, M., “Combination therapy: A new strategy to manage diabetes and its complications”, Phytomedicine 21:123-130 (2014).
14. Shilo, S., Aronis, A., Komarnitsky, R., Tirosh, O., “Selenite sensitizes mitochondrial permeability transition pore opening in vitro and in vivo: a possible mechanism for chemo-protection”, Biochem J, 370: 283-290, (2003).
15. Kara, H., Cevik, A., Konar, V., Dayangac, A., Yilmaz, M., “Protective effects of antioxidants against cadmium-induced oxidative damage in rat testes”. Biol Trace Elem Res, 120: 205–211, (2007).
16. Sharma, V., Sharma, A., Kansal, L., “The effect of oral administration of Allium sativum extracts on lead nitrate induced toxicity in male mice”, Food Chem Toxicol, 48: 928-936, (2010).
17. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., “Protein measurement with the Folin phenol reagent”, J Biol Chem., 19: 265, (1951).
18. Ohkawa, H., Ohishi, N., Yagi, K., “Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction”, Anal Biochem, 95: 351-358 (1979).
19. Marklund, S., Marklund, G.. “Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase”, Eur J Biochem, 47: 469-474, (1974).
20. Aebi, H., “Catalase in vitro”, Methods Enzymol, 105: 121-126. (1984).
21. Paglia, D.E., Valentine, W.N., “Studies on the quantitative and qualitative characterization of erythrocytes glutathione peroxidase”. J Lab Clin Med, 70: 158-165 (1967). [21] Habig, W.H, Pabst, M.J, Jakoby, W.B., “Glutathione-S-transferases: the first enzymatic step in mercapturic acid formation”. J Biol Chem, 249: 7130-7139 (1974).
22. Narayana, K., Al-Bader, M., “Ultrastructural and DNA damaging effects of lead nitrate in the liver”, Experimental and Toxicologic Pathology 63: 43-51, (2011).
23. Allouche, L., Hamadouche, M., Touabti, A., “Chronic effects of low lead levels on sperm quality, gonadotropins and testosterone in albino rats”, Experimental and Toxicologic Pathology 61: 503– 510, (2009).
24. Abdul-Hamid, M., Maoustafa, N., “Amelioration of alloxan-induced diabetic keratopathy by beta- carotene”, Experimental and Toxicologic Pathology 66, 49-59 (2014).
25. Morcillo, E.J., Estrela, J., Cortijo, J., “Oxidative stress and pharmacological intervention with antioxidants”, Pharmacol. Res. 40, 393–404, (1999).
26. Alireza, S., Leila, N., Siamak, S., Mahoammad- Hasan, K.A., Behrouz, I., “Effects of vitamin E on pathological changes induced by diabetes in rat luns”, Respiratory Physiology and Neurobiology 185: 593-599 (2013).
27. Shirpoor, A., Ansari, M.H., Salami, S., Pakdel, F.G., Rasmi, Y., “Effect of vitamin E on oxidative stress status in small intestine of diabetic rat”, World Journal of Gastroenterology 13: 4340-4344, (2007).
28. Durak, D., Kalender, S., Uzun, FG., Demir, F., Kalender, Y. “Mercury chloride-induced oxidative stress and the protective effect of vitamins C and E in human erythrocytes in vitro”. Afr J Biotechnol, 9 (4): 488–495 (2010).
29. Xu, Z., Wang, Z., Jian-Jun, L., Chen, C., Zhang, P., Dong, L., Chen, J., Chen, Q., Zhang, X., Wang, Z., “Protective effects of selenium on oxidative damage and oxidative stress related gene expression in rat live under chronic poisoning of arsenic”, Food Chem Toxicol 58: 1-7, (2013).
30. Khan, R.A., Khan, M.R., Sahreen, A., “Protective effect experimentally induced lung injuries in rats: A novel study”, Experimental and Toxicologic Pathology 64: 725-731 (2012). extracts against
31. Li, Q., Hu, X., Bai, Y., Alattar, M., Ma, D., Cao, Y., Hao, Y., Wang, L., Jiang, C., “The oxidative damage and inflammatory response induced by lead sulfide nanoparticles in rat lung”, Food Chem Toxicol 60: 213-217 (2013).
32. Baş H., Kalender Y.,” Chlorpyrifos induced cardiotoxicity in rats and the protective role of quercetin and catechin”, Gazi University Journal of Science 24(3):387-395 (2011).
33. Uzun, F.G., Kalender, Y., “Chlorpyrifos induced hepatotoxic and hematologic changes in rats: The role of quercetin and catechin”. Food Chem Toxicol 55:549-556, (2013).
34. Mykkanen, H.M., Humaloja, T., “Effect of lead on the intestinal absorption of sodium selenite and selenomethionine (75Se) in chicks”, Biol Trace Elem 6: Res 1-7, (1984).