The Investigation of the Effect of Topical Vascular Endothelial Growth Factor (VEGF) Administration On Serum Oxidative Parameters in Diabetic Rats

Year 2016, Volume: 29 Issue: 3, 543 - 547, 30.09.2016

Şule Coşkun Cevher Vildan Sönmez Çoban

Abstract

There is positiveeffect of exogenvascularendothelialgrowthfactor (VEGF) applicationtodiabeticwound.It is aimed to research the effects of topical VEGF administration on oxidative parameters in rat serum.In this study, 36 male wistar albino rats (weight: 200-250 g) were used. All rats get diabetic with streptozotocin. Dorsalateral excisional wounds (4 cm lengthy) were made on the two sides of medulla spinalis on the rats and they were randomly divided into 3 groups: untreated groups (n=12), chitosan treated (n=12)and chitosan+ VEGF (n=12)(7 ng/ml). In chitosan treated groups, wounds treated topically with equal amount blank chitosan gel. After this administration on the 3 th and 7 th days of wound,animals were sacrified. NOx, TBARS and RSH levels were measured spectrophotometrically in serum. Results were expressed as mean ± standart deviation. Mean differences were compared by Anova Variance Analysis(p<0,05). It can be thought that topical VEGF administration on the excisional wound of diabetic rats may have possitive effect on the recovery of oxidative damage in serum.

Keywords

VEGF, Diabetes mellitus, Wound.Healing, Oxidative.Stress, Antioxidant

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