MOLECULAR PHYLOGENETICS OF SOME ORCHIS SPECIES (ORCHIDACEAE) NATIVE TO TURKEY USING NUCLEAR AND CHLOROPLAST DNA SEQUENCES

Year 2017, Volume: 30 Issue: 2, 35 - 43, 19.06.2017

Ayten Dizkırıcı , Sinan İşler , Oktay Yiğit

Abstract

Phylogenetic relationships among nine Turkish Orchis species were inferred from variation in the internal transcribed spacer and maturase Kinase regions. Foreign sequences were also retrieved from NCBI to increase interspecific sampling. The topology of ITS tree was broadly congruent to that of matK tree. The trees showed two major clades; the first one included species from Orchis and Neotinea subgenera and the second included species of Anacamptis subgenus. Phylogenetic separation of some species found in Neotinea and Anacamptis subgenera were previously reported, and the current study also indicated that moving these species into Neotinea and Anacamptis genera could be reasonable.

Keywords

Anacamptis, Orchis; phylogeny; ITS; matK; Neotinea

References

• Atwood, J.T., “The size of the Orchidaceae and the systematic distribution of epiphytic orchids”, Selbyana, 9: 171-186, (1986).
• Peakall, R., “Speciation in the Orchidaceae: confronting the challenges”, Molecular Ecology, 16(14): 2855-66, (2007).
• Güler, N., “Orchis”, In: Güner, A., Aslan, S., Ekim, T., Vural, M., and Babac, M.T. (eds) A Checklist of the Flora of Turkey (Vascular Plants). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul, (2012).
• Özhatay, N., Europe’s Medicinal and Aromatic Plants: Their Use, Trade and Conservation. A TRAFFIC Network Report, TRAFFIC International: Cambridge, UK, (2000).
• Altundag, E. and Sevgi, E., “Vegetative anatomy of Orchis italica Poiret. (Orchidaceae) in Turkey”., XIII OPTIMA Meeting Proceedings, 22-26, (2010).
• Sevgi, E., Altundag, E., Kara, O., Sevgi, O., Tecimen, H.B. and Bolat, I., “Morphological and Ecological studies on some Orchis (Orchidaceae) taxa of Mediterranean region, Turkey”, Journal of Environmental Biology, 33: 343-353, (2012).
• Durmuşkahya, C., Akyol, Y. and Özdemir, C., “Ecology, anatomy and morphology of Orchis spitzelii in Turkey”, Journal of Environmental Biology, 36: 177-184, (2015).
• Durmuşkahya, C., Ozdemir, C., Bozdag, B. and Ozturk M., “Studies on the Morphology, Anatomy and Ecology of Ophrys lutea Cav. Subsp. Minor (Guss.) O. Danesch & E. Danesch ex Golz & H.R. Reinhard (Orchidaceae) in Turkey”, Pakistan Journal of Botany, 46: 81-88, (2014).
• Aceto, S., Caputo, P., Cozzolino, S., Gaudio, L. and Moretti, A., “Phylogeny and evolution of Orchis and allied genera based on ITS DNA variation: morphological gaps and molecular continuity”, Molecular Phylogenetics and Evolution, 13: 67–76, (1999).
• Aceto, S., Caputo, P., Gaudio, L., Nazzaro, R. and Cozzolino, S., “Molecular approach to the identification and characterization of natural hybrids between Orchis pauciflora and Orchis quadripunctata (Orchidaceae)”, Botanica Helvetica, 110: 31-39, (2000).
• Cozzolino, S., Aceto, S., Caputo, P., Nazzaro, R. and Gaudio, L., “Phylogenetic relationships in Orchis and some related genera: an approach using chloroplast DNA”, Nordic Journal of Botany, 18: 79-87, (1998).
• Cozzolino, S., Aceto, S., Caputo, P., Widmer, A. and Dafni, A., “Speciation processes in eastern Mediterranean Orchis s.l. species: molecular evidence and the role of pollination biology”, Israel Journal of Plant Sciences, 49: 91-103, (2001).
• Sramko, G., Molnar, V.A., Hawkins, J.A. and Bateman, R.M., “Molecular phylogeny and evolutionary history of the Eurasiatic orchid genus Himantoglossum s.l. (Orchidaceae)”, Annals of Botany, 114: 1609-1626, (2014).
• Steele, K.P. and Wojciechowski, M.F., Phylogenetic analyses of tribes Trifolieae and Vicieae, based on sequences of the plastid gene matK (Papilionoideae: Leguminosae). In: Klitgaard, B.B., Bruneau., A. (eds). Advances in Legume Systematics, Part 10: Higher Level Systematics. Kew, UK: Royal Botanic Gardens, pp. 355-370, (2003).
• Hedge, I.C. In: Davis P.H. (ed), Flora of Turkey and the East Aegean Islands: Orchis L., vol 7. University of Edinburgh Press, Edinburgh, (1982).
• Quentin, P., “Proposition de revision des genres Orchis, Aceras, Anacamptis et Neotinea”, Orchidophile, 31(143): 179-182, (2000).
• Doyle, J.J. and Doyle, J.L., “A rapid DNA isolation procedure for small quantities of fresh leaf tissue”, Phytochemical Bulletin, 19: 11-15, (1987).
• Hsiao, C., Chatterton, N.J., Asay, K.H. and Jensen, K.B., “Phylogenetic relationships of the monogenomic species of the wheat tribe, Triticeae(Poaceae), inferred from nuclear rDNA (internal transcribed spacer) sequences”, Genome, 38: 221-223, (1995).
• Li, J.H., Bogle, A.L. and Klein, A.S., “Interspecific relationships and genetic divergence of the disjunct genus Liquidambar (Hamamelidaceae)”, Rhodora, 99: 229-241, (1997).
• Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S., “MEGA 5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods”, Molecular Biology and Evolution, 28: 2731-2739, (2011).
• Tamura, K. and Nei, M. “Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees”, Molecular Biology and Evolution, 10: 512-526, (1993).
• Felsenstein, J., “Confidence limits on phylogenies: An approach using the bootstrap”, Evolution, 39: 783-791, (1985).
• Haider, N., Nabulsi, I. and Kamary, Y., “Phylogeny of Orchidaceae species in northwest Syria based on ISSRs”, Journal of Plant Biology Research, 1(2): 36-50, (2012).
• Bateman, R.M., Pridgeon, A.M. and Chase, M.W., “Phylogenetics of subtribe Orchidine (Orchidoideae, Orchidaceae) based on nuclear ITS sequences. 1. Infrageneric relationships and reclassification to achieve monophyly of Orchis sensu stricto”, Lindleyana, 12: 113-141, (1997).
• Bateman, R.M., Hollingsworth, P.M., Preston, J., Luo, Y.B., Pridgeon, A.M. and Chase M.W., “Molecular phylogenetics and evolution of Orchidinae and selected Habenariinae (Orchidaceae)”, Botanical Journal of the Linnean Society, 142: 1-40, (2003).