Some Biological Activities of the Moss Brachythecium populeum (Hedw.) Bruch, Schimp. & W.Gumbel (Bryophyta)

Yıl 2021, Cilt: 34 Sayı: 2, 327 - 333, 01.06.2021

Selda Kabadere Filiz Birgi Pınar Öztopçu Vatan Cansu Fılık Iscen Semra İlhan

https://doi.org/10.35378/gujs.750754

Cited By: 1

Öz

The present study assessed the antimicrobial and anticancer activities of Brachythecium populeum (Hedw.) Bruch, Schimp. & W.Gümbel (Brachytheciaceae) extracts using two different extracting methods. The antibacterial and antifungal activity of seven solvent extracts of Brachythecium populeum was examined against six species of bacteria and seven species of fungi by using well diffusion method. The minimal inhibitory concentration (MIC) of the extracts of acetone and C was determined. Extract C has the highest effect against E. faecalis (13 mm), B. subtilis and S. aureus (12 mm). The inhibition zone was showed by acetone extract against E. faecalis (13 mm), P. aeruginosa (10 mm) and B. subtilis (9 mm). Furthermore, all extracts exhibited different activities on P. aeruginosa. The MIC ranges acetone and C extracts against bacterial strains were from 93.8-375 μg/mL. Cytotoxic activities of extracts of acetone, A and C of B. populeum (0.17, 1.7, 17, 85 and 170 μg/mL) were tested against rat glioma cells by using MTT assay, after 24 and 48 h. Extracts of acetone, A and C showed a moderate toxicity, however high concentrations of extract A significantly decreased the survival of glioma cells in 48 h. The study confirms the antimicrobial and cytotoxic activities of B. populeum, extracted using various solvents. It is suggested that the active substances to be obtained by using different solvents from B. populeum may be the active substances of various drugs in the future. Further studies are needed.

Anahtar Kelimeler

Brachythecium populeum, Moss, Extract, Antimicrobial activity, Cytotoxicity

Destekleyen Kurum

Eskişehir Osmangazi Üniversitesi

Proje Numarası

2019/19D09

Teşekkür

This study’s cytotoxic effect part was supported by Eskisehir Osmangazi University Scientific Research Projects Committee (Project No:2019/19D09).

Kaynakça

• [1] Uyar, G., Çetin, B., "A new check-list of the mosses of Turkey", Journal of Bryology, 26: 203-220, (2004).
• [2] Klavina, L., Springe, G., Nikolajeva, V., Martsinkevich, I., Nakurte, I., Dzabijeva, D., Steinberga, I., "Chemical composition analysis, antimicrobial activity and cytotoxicity screening of moss extracts (Moss Phytochemistry)", Molecules, 20: 17221–17243, (2015).
• [3] Karpınski T.M., Adamczak A., "Antibacterial activity of ethanolic extracts of some moss species" Herba Polonica, 63: 11-17, (2017).
• [4] Saxena, D. K., Harinder , "Uses of bryophytes", Resonance, 9: 56–65, (2004).
• [5] Asakawa, Y., "Biologically active compounds from bryophytes", Pure and Applied Chemistry, 79: 557–580, (2007).
• [6] Asakawa, Y., Ludwiczuk, A., Nagashima, F., "Phytochemical and biological studies of bryophytes", Phytochemistry, 91: 52–80, (2013).
• [7] Erturk, O., Sahin, H., Ertürk, E.Y., Hotaman, H. E., Koz, B., Ozdemir, O., "The antimicrobial and antioxidant activities of extracts obtained from some moss species in Turkey", Herba Polonica, 61: 52–65, (2016).
• [8] Oztopcu-Vatan, P., Savaroglu, F., Iscen, C. F., Kabadere, S., Ozturk, N., Ilhan, S., "Screening of antimicrobial, cytotoxic effects and phenolic compounds of the moss Aulacomnium androgynum (Hedw.) schwagr (Bryophyta)", Journal of Animal and Plant Sciences, 27: 1909–1917, (2017).
• [9] Savaroglu, F., Oztopcu-Vatan, P., Kabadere, S., Ozturk, N., Ilhan, S., Iscen C.F., "Screening of antimicrobial, cytotoxic effects and phenolic compounds of the moss Dicranum scoparium", Biological Diversity and Conservation, 11: 87–94, (2018).
• [10] Jones, W. P., Kinghorn, A. D., "Extraction of plant secondary metabolites", Methods in Molecular Biology, 864: 341–366., (2012).
• [11] Clinical and Laboratory Standards Institute. M44-A2: Method for Antifungal Disk Diffusion Susceptibility Testing of Yeasts; Approved Guideline—Second Edition. CLSI. M44-A2: 29(17), (2009).
• [12] Clinical and Laboratory Standards Institute. M02-A12: Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard—Twelfth Edition. CLSI. 35: 73, (2015).
• [13] Mosmann, T., "Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays", Journal of Immunological Methods, 65: 55–63, (1983).
• [14] Vollár, M., Gyovai, A., Szucs, P., Zupkó, I., Marschall, M., Csupor-Lffler, B., Berdi, P., Vecsernyes, A., Csorba, A., Liktor-Busa, E., Urban, E., Csupor, D., "Antiproliferative and antimicrobial activities of selected bryophytes",Molecules, 23(7): 1520, (2018).
• [15] Yayintas, O. T., Yapici, B. M., "in vitro antimicrobial activity of Brachythecium campestre and Eurhynchium pulchellum extracts", Asian Jornal of Chemistry, 21: 2193–2197, (2009).
• [16] Singh, M., Rawat, A. K. S., Govindarajan, R., "Antimicrobial activity of some Indian mosses", Fitoterapia, 78: 156–158, (2007).
• [17] Fu, P., Lin, S., Shan, L., Lu, M., Shen, Y.H., Tang, J., Liu, R.H., Zhang, X., Zhu, R.L., Zhang, W.D., "Constituents of the moss Polytrichum commune", Journal of Natural Products, 72: 1335–1337, (2009).
• [18] Krzaczkowski, L., Wright, M., Rebérioux, D., Massiot, G., Etiévant, C., Gairin, J.E., "Pharmacological screening of bryophyte extracts that inhibit growth and induce abnormal phenotypes in human HeLa cancer cells", Fundamental and Clinical Pharmacology, 23: 473–482, (2009).
• [19] Komala, I., Ito, T., Nagashima, F., Yagi, Y., Asakawa, Y., "Cytotoxic bibenzyls, and germacrane- and pinguisane-type sesquiterpenoids from Indonesian, Tahitian and Japanese liverworts", Natural Product Communications, 6: 303–309, (2011).
• [20] Savaroǧlu, F., Filik-Işçen, C., Öztopcu-Vatan, A. P., Kabadere, S., Ilhan, S., Uyar, R., "Determination of antimicrobial and antiproliferative activities of the aquatic moss Fontinalis antipyretica Hedw", Turkish Journal of Biology., 35: 361–369, (2011).
• [21] Oztopcu-Vatan, P., Savaroǧlu, F., Iscen, C. F., Kabadere, S., Ilhan, S., Uyar, R., "Antimicrobial and antiproliferative activities of Homalothecium sericeum (Hedw.)Schimp. extracts", Fresenius Environmental Bulletin, 20: 461–466, (2011).