Spectrofluorimetric Determination of Diiodohydroxyquinoline in Presence of Metronidazole in Pharmaceutical Formulation and Spiked Human Plasma

Yıl 2013, Cilt: 26 Sayı: 2, 141 - 149, 05.07.2013

Y Aboul-eneın Nouruddin Alı Mohammed Gamal Mohammed Abdelkawy

Öz

Accurate and sensitive spectrofluorimetric method was developed for determination of diiodohydroxyquinoline in presence of metronidazole. In this method the native fluorescence of diiodohydroxyquinoline in water solvent at λ =495 nm when excitation was at 250 nm is used for its determination. Linear correlation was obtained in theconcentration range of 400 to 900 ng mL-1. The proposed method was successfully applied for determination ofdiiodohydroxyquinoline in bulk powder with mean accuracy of 100.21±1.13 or in spiked human plasma with mean accuracy of 100.53±1.42 without interference of metronidazole.

Anahtar Kelimeler

spectrofluorimetry, Diiodohydroxyquinoline, Metronidazole

Kaynakça

• 1. Merck Index: An Encyclopedia of Chemicals, Drugs and Biological 14th Ed., S. Budavari (Ed.), Merck & Co., Inc., Whitehouse Station, NJ(2006).
• 2. Martindale: The Extra Pharmacopoeia, the Complete Drug Reference 35th Ed., K. Profitt (Ed.), Royal Pharmaceutical Society, London, UK(2006).
• 3. United States Pharmacopeia "The National Formulary USP", United States Pharmacopeia convention Inc (2009).
• 4. Nagaraja, P, Sunitha, K R, Vasantha RA, Yathirajan H.S., Spectrophotometric determination of metronidazole and tinidazole in pharmaceutical preparations. Journal of Pharmaceutical and Biomedical Analysis 28(3-4), 527–535 (2002).
• 5. Erk N, Altun M L N., Spectrophotometric resolution of metronidazole and miconazole nitrate in ovules using ratio spectra derivative spectrophotometry and RPLC.Journal of Pharmaceutical and Biomedical Analysis, 25(1)115-122 (2001).
• 6. Khashaba PY, Refaat I H, Emara K M and Gaber H M. , Utility of Diazotized 4 - Amino-6-Chloro-1,3- Benzene Disulphonamide for the Spectrophotometric Determination of 8-Hydroxquinolines and Dibenzazepines.Analytical Letters, 32(15) 3029-3047 (1999).