A New Laccase-Based Biosensor for Epinephrine Determination

Yıl 2015, Cilt: 28 Sayı: 1, 1 - 9, 23.02.2015

Fatma Arslan , Selma Durmuş Özlem Çolak Halit Arslan

Öz

In this paper, a novel amperometric epinephrine (adrenaline) biosensor with immobilization of laccase on polypyrrole–polyvinylsulphonate (PPy-PVS) film has been a complished. Laccase enzyme were immobilized on PPy-PVS film by cross-linking with glutaraldehyde. Determination of EP was carried out by the reduction of enzymatically produced epinephrinequinone at - 0.220 V vs. Ag/AgCl. The effects of pH and temperature were investigated. There are two linear parts in the region between 0.1 µM-1.0 µM and 1.0 µM-10.0 µM. The storage stability and operation stability of the enzyme electrode were also studied. Interference effects were investigated on response of the biosensor.

Anahtar Kelimeler

Epinephrine, laccase, biosensor, polypyrrole (PPy), polyvinylsulphonate (PVS)

Kaynakça

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