Antibiotic Resistance and Slime Production in Coagulase-Negative Staphylococcus Strains from Retail Meat Samples

Year 2015, Volume: 28 Issue: 3, 359 - 364, 27.02.2015

Sumru Çıtak , Meryem Külahcı Şükriye Öcal

Abstract

In this study, a total of 100 retail meat samples were analysed for the presence of coagulase negative Staphylococcus (CoNS). A total of 208 CoNS strains were isolated from these samples. Staphylococcus saprophyticus was the most prevalent species. Resistance to methicillin was detected in 63.4% of CoNS isolates. Methicillin-resistant CoNS (MRCoNS) strains were determined to be more resistant to antibiotics than methicillin-susceptible CoNS strains. Resistance of methicillin was found in 76.1% of slime positive and in 57.4% of slime negative strains. Most of isolated slime producing CoNS were methicillin resistant, which makes the detection of these microorganisms necessary to prevent their dissemination in the environment, veterinary medicine and public health.

Keywords

Antibiotic resistance, slime, CoNS, retail meat

References

• Kogan, G., Sadovskaya, I., Chaignon, P., Chokr, A. and Jabbouri, S., “Clinical strains of Staphylococcus that do not contain polysaccharide intercellular adhesin”, FEMS Microbiol Lett., 255: 11–16, (2006).
• Kloos, W.E. and Scheifer, K.H., Genus IV. Staphylococcus, Rosenbach 1884.In: Sneath, PHA, Mair NS, Sharpe ME, Holt JG.(Eds). Bergey’s Manual of Systematic Bacteriology. Vol 2. Williams &Wilkins, Baltimore, 1013-1035, (1986).
• Citak, S. and Duman, T. “Staphylococcus aureus and coagulase-negative Staphylococcus from raw chicken samples in Turkey: Prevalence and antimicrobial resistance”, J. Food Agriculture Environ., 9: 156-158, (2011).
• Raz, R., Colodner, R. and Kunin, C., “Who Are You—Staphylococcus saprophyticus?”, Clin. Infect. Dis., 40: 896-898, (2005).
• Agvald-Ohman, C., Lund, B. and Edlund, C., “Multiresistant coagulase-negative Staphylococci disseminate frequently between incubated patients in a multidisciplinary intensive care unit”, Crit. Care, 8: 42-47, (2004).
• Swartz, M.N., “Human diseases caused by foodborne pathogens of animal origin”, Clin. Infect. Dis., 34: 111-122, (2002).
• Gotz, F., “Staphylococcus and biofilms”, Mol. Microbiol., 43:1367-1378, (2002).
• Moodley, A. and Guardabassi, L., “Clonal spread of methicillin-resistant coagulase-negative Staphylococci among horses, personel and environmental sites at eqine facilities”, Vet. Microbiol., 137: 397- 401, (2009).
• Al-Tarazi, Y.H., Albetar, M.A. and Alaboudi, A.R., “Biotyping and enterotoxigenicity of Staphylococci isolated from fresh and frozen meat marketed in Jordan”, Food Research Int., 42: 374-379, (2009).
• Mathew, A.G., Cissell,. and Liamthong, S., “Antibiotic resistance in bacteria associated with food animals: a United States perspective of livestock production”, Foodborne Pathog. Dis., 4: 115–133, (2007).
• Perreten, V., Schwarz , F., Cresta, L., Boeglin, M., Dasen, G. and Teuber, M., “Antibiotic resistance spread in food”, Nature, 389: 801-802, (1997).
• Kawano, J., Shimizu, A., Saitoh, Y., Yagi, M., Saito, T. and Okamoto, R., “Isolation of methicillin-resistant coagulase-negative staphylococci from chickens”, J. Clin. Microbiol., 34: 2072–2077, (1996).
• Maran ., “Monitoring of antimicrobial resistance and antibiotic usage in animals in the Netherlands in 2005”, (2005). Available at: http://www.cidc-lelystad.wur.nl/UK/publications/
• Rather, P.N., Davis, A.P. and Wilkinson, B.J., “Slime production by bovine milk Staphylococcus aureus and identification of coagulase-negative staphylococcal isolates”, J. Clin. Microbiol., 23: 858-862, (1986).
• Clinical and Laboratory Standart Institue (CLSI). Performance Standart for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. CLSI document, M100-S16.vol.26-3; M7-A7, vol.26-2; M2-A9,vol. 26-1.Wayne, PA. USA, (2006).
• Knauer, A., Fladerer, P., Strempfl, C., Krause, R. and Wenisch, C., “Effect of hospitalization and antimicrobial therapy an antimicrobial resistance of colonizing Staphylococcus epidermidis”, Wien Klin. Wochenschr, 16: 489-494, (2004).
• Turutoglu, H., Ercelik, S. and Ozturk, D., “Antibiotic resistance of Staphylococcus aureus and coagulase- negative staphylococci isolated from bovine mastitis”, Bull. Vet. Inst. Pulawy., 50: 41-45, (2006).
• Simeoni, D., Rizzotti, L., Cocconcelli, P., Gazzola, S., Dellaglio, F. and Torriani, S., “Antibiotic resistance genes and identification of staphylococci collected from the production chain of swine meat commodities”, Food. Microbiol., 25: 196-201, (2008).
• Erginkaya, S. and Filazi, A., “Determination of antibiotic residues in milk samples”, Kafkas University, The J. Vet. Faculty, 16(Supp-A): 31-35, (2010).
• Rich, M., Deighton, L. and Roberts, L., “Clindamycin-resistance in methicillin-resistant Staphylococcus aureus isolated from animals”, Vet. Microbiol., 111: 237-240, (2005).
• Virdis, S., Scarano, C., Cossu, F., Spanu, V., Spanu, C. and Luigi De Santis, E.P., “Antibiotic Resistance in Staphylococcus aureus and Coagulase Negative Staphylococci Isolated from Goats with Subclinical Mastitis”, Vet. Med. Int., Article ID 517060, 1-6, (2010).
• Palazzo, I.C.V., Araujo, M.L.C. and Darini, A.L.C., “First report of vancomycin –resistant Staphylococci isolated from healty carriers in Brazil”, J. Clin. Microbiol., 43: 179-185, (2005).
• Witte, W., Cuny, C., Klare, I., Nubel, U., Strommenger, B. and Werner, G., “Emergence and spread of antibiotic-resistant Gram positive bacterial pathogens”, Int. Medical Microbiol., 298 ( 5-6): 365-377, (2008).
• Jain, A. and Agarwal, A., “Biofilm production, a marker of pathogenic potential of colonizing and commensal staphylococci”, J. Microbiology Met.,76: 88-92, (2009).
• Mack, K.D., Bartscht, S., Dobinsky, M.A., Horskotte, H., Kiel, K.M., Knobloch, A. and Schafer, P., Staphylococcal factors involved in adhesion and biofilm formation on biomaterials. In YH An, RJ Friedman (eds) Handbook of Bacterial Adhesion, Principles Methods and Application, Humana press, Totowa, NJ: 307-330, (2000).
• Koksal, F., Yasar, H., Samasti, M.,“Antibiotic resistance patterns of coagulase-negative Staphylococcus strains isolated from blood cultures of septicemic patients in Turkey”, Microbiol. Research, 16: 404-410, (2009).
• Citak, S., Varlik, O. and Gundogan, N., “Slime production and DNase activity of staphylococci isolated from raw milk”, J. Food Safety, 23: 281-288, (2003).
• Amorena, B., Gracia, E., Monzon, M., Leiva, J., Oteiza, C., Perez, M., Alabart, J.L. and Hernandez-Yago, J., “Antibiotic susceptibility assay for Staphylococcus aureus in biofilms developed in vitro”, J. Antimicrob. Chemother, 44: 43-55, (1999).
• Gunaydin, M., Leblebicioglu, H., Sanic, A. and Pirinciler, M., “Koagulaz negatif stafilokoklarda slime yapimi ve antibiyotik direnci ile ilişkisi”, Microbiology Bulltein, 105: 493-500, (1995).