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CoFe2O4-Chitosan and Gold Nanoparticles Based Label-Free Electrochemical Immunosensor for Determination of Leptin

Year 2024, Volume: 37 Issue: 4, 1674 - 1689, 01.12.2024

Günseli Birge , Derya Koyuncu Zeybek

https://doi.org/10.35378/gujs.1422409

Abstract

Herein, a label-free electrochemical leptin immunosensor was demonstrated. The sensing platform consists of the immobilizing of the anti-leptin antibody on a glassy carbon electrode (GCE) modified with cobalt iron oxide (CoFe2O4) nanoparticles, chitosan (CHI), and gold nanoparticles (AuNPs). A simple and rapid leptin determination was achieved by measuring the change of current response in a redox probe solution before and after the immunocomplex formation. SEM examined the surface morphologies of the prepared electrodes. The electrochemical performance of the leptin immunosensor was commented on via EIS, CV, and DPV. Under optimized circumstances, a linear response was found between the current peaks acquired from DPV and the logarithm concentration of leptin in the range of 1─4000 ng mL-1 with a detection limit (LOD) of 0.1 ng mL-1. The subjected immunosensor demonstrated satisfactory reproducibility.

Keywords

Chitosan Cobalt iron oxide Gold nanoparticles Immunosensor Leptin

References

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Year 2024, Volume: 37 Issue: 4, 1674 - 1689, 01.12.2024

Günseli Birge , Derya Koyuncu Zeybek

https://doi.org/10.35378/gujs.1422409

Abstract

References

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  • [2] Peng, J., Yin, L., and Wang, X., “Central and peripheral leptin resistance in obesity and improvements of exercise”, Hormones and Behavior, 133: 105006, (2021).
  • [3] Pronk, N.P., Eneli, I., Economos, C.D., Bradley, D., Fassbender, J., Calancie, L., Patawaran, W., and Hovmand, P.S., “Using systems science for strategic planning of obesity prevention and treatment: the roundtable on obesity solutions experience”, Current Problems in Cardiology, 48(8): 101240, (2023). [4] Yadav, R., Swetanshu, and Singh, P., “The molecular mechanism of obesity: The science behind natural exercise yoga and healthy diets in the treatment of obesity”, Cardiology Research and Practice, 49(2): 102345, (2024).
  • [5] Yu, M., Ju, M., Fang, P., and Zhang, Z., “Emerging central and peripheral actions of spexin in feeding behavior, leptin resistance and obesity”, Biochemical Pharmacology, 202: 115121, (2022).
  • [6] Chrysafi, P., Perakakis, N., Farr, O.M., Stefanakis, K., Peradze, N., Sala-Vila, A., and Mantzoros, C.S., “Leptin alters energy intake and fat mass but not energy expenditure in lean subjects”, Nature Communications, 11: 5145, (2020).
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  • [13] Cai, J., Gou, X., Sun, B., Li, W., Li, D., Liu, J., Hu, F., and Li, Y., “Porous graphene-black phosphorus nanocomposite modified electrode for detection of leptin”, Biosensors Bioelectronics, 137: 88–95, (2019).
  • [14] Naresh, V., Lee, N., “A review on biosensors and recent development of nanostructured materials-enabled biosensors”, Sensors, 21(4): 1109, (2021).
  • [15] Lino, C., Barrias, S., Chaves, R., Adega, F., Martins-Lopes, P., and Fernandes, J.R., “Biosensors as diagnostic tools in clinical applications”, Biochimica et Biophysica Acta (BBA) - Reviews on Cancer 1877(3): 188726, (2022).
  • [16] Chen, H., Zhang, J., Huang, R., Wang, D., Deng, D., Zhang, Q., and Luo, L., “The applications of electrochemical immunosensors in the detection of disease biomarkers: a review”, Molecules, 28(8): 3605, (2023).
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  • [20] Andryukov, B.G., Besednova, N.N., Romashko, R. V, Zaporozhets, T.S., and Efimov, T.A. “Label-free biosensors for laboratory-based diagnostics of infections: current achievements and new trends”, Biosensors (Basel) 10(2):11, (2020).
  • [21] Patel, M., Agrawal, M., and Srivastava, A., “Signal amplification strategies in electrochemical biosensors via antibody immobilization and nanomaterial-based transducers”, Materials Advances, 3: 8864-8865, (2022).
  • [22] Al-Amiery, A.A., Fayad, M.A., Abdul Wahhab, H.A., Al-Azzawi, W.K., Mohammed, J.K., and Sh. Majdi, H., “Interfacial engineering for advanced functional materials: surfaces, interfaces, and applications”, Results in Engineering, 22: 102125, (2024).
  • [23] Wang, D., Astruc, D., “Fast-growing field of magnetically recyclable nanocatalysts”, Chemical Reviews, 114: 6949–6985, (2014).
  • [24] Yalcin, B., Ozcelik, S., Icin, K., Senturk, K., Ozcelik, B., and Arda, L., “Structural, optical, magnetic, photocatalytic activity and related biological effects of CoFe2O4 ferrite nanoparticles”, Journal of Materials Science: Materials in Electronics 32: 13068–13080, (2021).
  • [25] Liu, P., Li, C., Zhang, R., Tang, Q., Wei, J., Lu, Y., and Shen, P., “An ultrasensitive electrochemical immunosensor for procalcitonin detection based on the gold nanoparticles-enhanced tyramide signal amplification strategy”, Biosensors and Bioelectronics, 126: 543–550, (2019). [26] Malinowska, I., Ryżyńska, Z., Mrotek, E., Klimczuk, T., and Zielińska-Jurek, A., “Synthesis of CoFe2O4 nanoparticles: the effect of ionic strength, concentration, and precursor type on morphology and magnetic properties”, Journal of Nanomaterials, 2020: 9046219, (2020).
  • [27] Montes-García, V., Squillaci, M.A., Diez-Castellnou, M., Ong, Q.K., Stellacci, F., and Samorì, P., “Chemical sensing with Au and Ag nanoparticles”, Chemical Society Reviews, 50: 1269–1304, (2021).
  • [28] Wu, B., Yeasmin, S., Liu, Y., and Cheng, L.-J., Sensitive and selective electrochemical sensor for serotonin detection based on ferrocene-gold nanoparticles decorated multiwall carbon nanotubes”, Sensors and Actuators B: Chemical, 354: 131216, (2022).
  • [29] Pellis, A., Guebitz, G.M., and Nyanhongo, G.S., “Chitosan: sources, processing and modification techniques”, Gels, 8(7): 393, (2022).
  • [30] Román-Doval, R., Torres-Arellanes, S.P., Tenorio-Barajas, A.Y., Gómez-Sánchez, A., and Valencia-Lazcano, A.A., “Chitosan: properties and its application in agriculture in context of molecular weight”, Polymers, 15(13): 2867, (2023).
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  • [34] Lin, W.R., Chuang, Y.J., Lee, C.H., Tseng, F.G., and Chen, F.R., “Fabrication and characterization of a high-performance multi-annular backscattered electron detector for desktop SEM”, Sensors, 18: 3093, (2018).
  • [35] Thalir, S., Celshia Susai, S., Selvamani, M., Suresh, V., Sethuraman, S., and Ramalingam, K., “Sensing of quercetin with cobalt-doped manganese nanosystems by electrochemical method”, Cureus, 16(3): e56665, (2024).
  • [36] Katowah, D.F., Hussein, M.A., Alam, M.M., Gabal, M.A., Sobahi, T.R., Asiri, A.M., Uddin, J., and Rahman, M.M., “Selective fabrication of an electrochemical sensor for Pb2+ based on poly(pyrrole-co-o–toluidine)/CoFe2O4 nanocomposites, ChemistrySelect, 4: 10609–10619, (2019).
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There are 47 citations in total.

Details

Primary Language English
Subjects Sensor Technology
Journal Section Chemistry
Authors

Günseli Birge 0000-0002-6717-5353

Derya Koyuncu Zeybek 0000-0003-4214-1744

Early Pub Date September 6, 2024
Publication Date December 1, 2024
Submission Date January 19, 2024
Acceptance Date May 6, 2024
Published in Issue Year 2024 Volume: 37 Issue: 4

Cite

APA Birge, G., & Koyuncu Zeybek, D. (2024). CoFe2O4-Chitosan and Gold Nanoparticles Based Label-Free Electrochemical Immunosensor for Determination of Leptin. Gazi University Journal of Science, 37(4), 1674-1689. https://doi.org/10.35378/gujs.1422409
AMA Birge G, Koyuncu Zeybek D. CoFe2O4-Chitosan and Gold Nanoparticles Based Label-Free Electrochemical Immunosensor for Determination of Leptin. Gazi University Journal of Science. December 2024;37(4):1674-1689. doi:10.35378/gujs.1422409
Chicago Birge, Günseli, and Derya Koyuncu Zeybek. “CoFe2O4-Chitosan and Gold Nanoparticles Based Label-Free Electrochemical Immunosensor for Determination of Leptin”. Gazi University Journal of Science 37, no. 4 (December 2024): 1674-89. https://doi.org/10.35378/gujs.1422409.
EndNote Birge G, Koyuncu Zeybek D (December 1, 2024) CoFe2O4-Chitosan and Gold Nanoparticles Based Label-Free Electrochemical Immunosensor for Determination of Leptin. Gazi University Journal of Science 37 4 1674–1689.
IEEE G. Birge and D. Koyuncu Zeybek, “CoFe2O4-Chitosan and Gold Nanoparticles Based Label-Free Electrochemical Immunosensor for Determination of Leptin”, Gazi University Journal of Science, vol. 37, no. 4, pp. 1674–1689, 2024, doi: 10.35378/gujs.1422409.
ISNAD Birge, Günseli - Koyuncu Zeybek, Derya. “CoFe2O4-Chitosan and Gold Nanoparticles Based Label-Free Electrochemical Immunosensor for Determination of Leptin”. Gazi University Journal of Science 37/4 (December 2024), 1674-1689. https://doi.org/10.35378/gujs.1422409.
JAMA Birge G, Koyuncu Zeybek D. CoFe2O4-Chitosan and Gold Nanoparticles Based Label-Free Electrochemical Immunosensor for Determination of Leptin. Gazi University Journal of Science. 2024;37:1674–1689.
MLA Birge, Günseli and Derya Koyuncu Zeybek. “CoFe2O4-Chitosan and Gold Nanoparticles Based Label-Free Electrochemical Immunosensor for Determination of Leptin”. Gazi University Journal of Science, vol. 37, no. 4, 2024, pp. 1674-89, doi:10.35378/gujs.1422409.
Vancouver Birge G, Koyuncu Zeybek D. CoFe2O4-Chitosan and Gold Nanoparticles Based Label-Free Electrochemical Immunosensor for Determination of Leptin. Gazi University Journal of Science. 2024;37(4):1674-89.