The surface imprinted technique was utilized in creating the synthetic lysozyme receptors on the poly(hydroxyethyl methacrylate) [p(HEMA)] cryogel membrane with the assistance of polydopamine under alkaline conditions. Following the design of the artificial receptors, Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and the swelling test were used for the characterization studies and the lysozyme adsorption capability of the adsorbent was evaluated in the aqueous solution.
The maximum adsorption capacity (Qmax) of lysozyme was found as 108.9 mg/g and the adsorption of the target protein on the adsorbent was monolayer and showed a good fit to the Langmuir isotherm model. The IF factor was 3.26 and the adsorbent was highly selective against creatinine and bovine serum albumin (BSA). Furthermore, the adsorbent maintained its stability after 5 adsorption, desorption, and regeneration cycles.
lysozyme adsorption molecular imprinting p(HEMA) dopamine cryogel.
Dr. Aykut Arif Topçu thanks Prof. Dr. Adil Denizli for his kind help and valuable contributions.
lysozyme adsorption molecular imprinting p(HEMA) dopamine cryogel.
Birincil Dil | İngilizce |
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Bölüm | Research Articles |
Yazarlar | |
Yayımlanma Tarihi | 31 Mart 2023 |
Gönderilme Tarihi | 24 Ocak 2023 |
Yayımlandığı Sayı | Yıl 2023 |
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