The Preventive Effect of Thymoquinone and Ceratonia Siliqua L. in Experimental Asthmatic Pregnant Rats: Histologically and Immunohistochemically Evaluation

Yıl 2019, , 215 - 222, 30.09.2019

Ameerah Fadhil Ahmed Sule Coskun Cevher

https://doi.org/10.17350/HJSE19030000150

Cited By: 2

Öz

T here are no studies yet have been compared pregnant rat asthma models by assessing the airway both histologically and immunohistochemically under the effect of Thymoquinone TQ and Ceratonia siliqua CS Carob . To compare asthmatic pregnant models with TQ and CS by studying changes of both the histologically and the immunohistochemically of lung tissue using light microscopy. 18 female wistar rats were randomly split to 3 groups: asthmatic pregnant group I sensitized by intraperitoneal ovalbumin injection OVA with alum on days 0 and 14 and submitted to OVA aerosolized 3 days in excess of the next 1 week then they coupled with male rats to get pregnancy; asthmatic pregnant with TQ and CS group II sensitized as above then they administered each of TQ and CS on the last 5 days of pregnancy; and asthmatic pregnant with dexamethasone group III that received dexamethasone by intraperitoneal injection on last 5 days of pregnancy.Lung tissue immunohistochemically and histologically were evaluated. Various histological changes in lung tissues of group I were revealed. However, treatment of TQ and CSwas prevented these changes in group II. Immunohistochemically, vascular endothelialgrowth factor VEGF staining levels increase was observed in group I, while there was asignificant decrease in group II than it is in group III. In this study, the use of TQ and CS has been shown to alleviate the histological changes and immunohistochemically causedby asthma. In conclusion, TQ and CS was thought to be a promising treatment agent forasthma in the future especially during pregnancy. The use of TQ and CS in people withnormal asthma may be used as a new promising supplement to eliminate the negative effects of asthma

Anahtar Kelimeler

Rats, Asthma, Remodeling, VEGF, Nebulized ovalbumin, Thymoquinone, Ceratonia siliqua.

Kaynakça

• 1. Hansel TT, Barnes PJ. Novel drugs for treating asthma. Curr Allergy Asthma Rep 2001;1:164-73.
• 2. Tagaya E, Tamaoki J. Mechanisms of airway remodeling in asthma. Allergol Int. 2007;56:331-40.
• 3. Herz U, Renz H, Wiedermann U. Animal models of type I allergy using recombinant allergens. Methods. 2004 ;32:271-80.
• 4. Zosky GR, Sly PD. Animal models of asthma.Clin Exp Allergy. 2007;37:973-88.
• 5. Ramos-Barbón D, Ludwig MS, Martin JG. Airway remodeling: lessons from animal models. Clin Rev Allergy Immunol. 2004;27:3- 21.
• 6. Arm JP, Lee TH. The pathobiology of bronchial asthma. Adv Immunol 1992; 51:323-82.
• 7. Gleich GJ, Adolphson CR. The eosinophilic leukocyte: structure and function. Adv Immunol 1986; 39:177-253.
• 8. Sumi Y, Hamid Q. Airway remodeling in asthma. Allergol Int 2007;56:341-8.
• 9. Atta-ur-Rahman, Malik S, Ahmed S, Choudhary MI, Habib-urRehman. Nigellimine-N-oxide A new isoquinoline alkaloid from the seeds of Nigella sativa. Heterocycles 1985; 23:953-5.
• 10. Woo CC, Kumar AP, Sethi G, Tan KH. Thymoquinone: Potential Cure for Inflammatory Disorders and Cancer. Biochem Pharmacol 2012; 83: 443-51.
• 11. Ammar el SM, Gameil NM, Shawky NM, Nader MA. Comparative evaluation of anti-inflammatory poperties of thymoquinone and curcumin using an asthmatic murine model. Int Immunopharmacol 2011; 11: 2232-6.
• 12. El Gazzar M, El Mezayen R, Marecki JC, Nicolls MR, Canastar A, Dreskin SC. Anti-inflammatory effect of thymoquinone in a mouse model of allergic lung inflammation. Int Immunopharmacol 2006; 6: 113542.
• 13. Al-Gahmdi MS. The anti-inflammatory, analgesic and antipyretic activity of Nigella sativa. J Ethnopharmacol 2001;76:45–8.
• 14. Sidina MM., El Hansali M, Wahid N, Ouatmane A, Boulli A, Haddioui A. Fruit and seed diversity of domesticated carob (Ceratonia siliqua L.) in Morocco. Scientia Horticulturae. 2009; 123: 110-116.
• 15. Luthria D. Significance of sample preparation in developing analytical methodologies for accurate estimation of bioactive compounds in functional foods. Journal of the Science of Food and Agriculture2006; 86: 2266-2272.
• 16. Anonim. Ulusal Gida Kompozisyon Veri Tabanı. 2017; www. turkomp.gov.tr/food/376 (Access Date: 11.07. 2017).
• 17. Youssef, M. K. E., El-Manfaloty, M. M., & Ali, H. M. Assessment of proximate chemical composition, nutritional status, fatty acid composition and phenolic compounds of carob (Ceratonia siliqua L.). Healthcare Foodservice Magazine. 2013; 3, 304–308.
• 18. Karhan, M., H. Gubbuk, İ. Turhan, H. R. Öziyci, H. Akgül, K. Uçgun. Türkiye’de yetişen keçiboynuzu (Ceratonia siliqua L.) tiplerinin biyoaktif birmolekülolan D-pinitol içeriği üzerine çevre koşulları ve bileşim unsurlarının etkisi. 2010. TÜBİTAK Projesi Proje No: 107O650.
• 19. Alper, Y. Keçiboynuzu (Ceratonia siliqua L.) meyvesinden süperkritik karbondioksit (CO2) ekstraksiyonu ile d-pinitol eldesi. Yüksek lisans tezi. E. Ü. Fen Bilimleri Enstitüsü Gıda Mühendisliği Anabilim Dalı Bornova- İzmir. 2016.
• 20. Davis, A., M. Christiansen, J. F. Horowitz, K. M. Hellerstein, and E. R. Ostlund. Effect of pinitol treatment on insulin action in subjects with insulin resistance. Diabetes Care. 2000; 23: 1000-1005.
• 21. Kim, J.I., Kim, J.C., Kang, M.J., Lee, M.S., Kim, J.J., Cha, I.J. Effects of Pinitol isolated from soybeans on glycemic control and cardiovascular risk factors in Korean patients with type 2 diabetes mellitus: a randomized controlled study. European Journal of Clinical Nutri. 2005; tion .59: 456-458.
• 22. Kovacic P, Jacintho JD. Mechanisms of carcinogenesis: Focus on oxidative stress and electron transfer. Current Medicinal Chemistry2001; 8: 773-796.
• 23. Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. Free Radicals and Antioxidants in Normal Physiological Functions and Human Disease. The International Journal of Biochemistry & Cell Biology. 2007; 39: 44-84.
• 24. Gulcin I, Beydemir S. Phenolic compounds as antioxidants: carbonic anhydrase isoenzymes inhibitors. Mini Rev Med Chem2013; 13 (3): 408-430.
• 25. De Zwart LL, Meerman JHN, Commandeur JNM, Vermeulen NPE. Biomarkers of free radical damage applications in experimental animals and in humans. Free Radical Biology & Medicine. 1999; 26: 202-226.
• 26. Yu LL, Zhou KK, Parry J. Antioxidant properties of cold pressed black caraway, carrot, cranberry, and hemp seed oils. Food Chemistry. 2005; 91: 723-729.
• 27. Xie, Q.M., X. Wu, H.M. Wu, Y.M. Deng, S.J. Zhang, J.P. Zhu, X.W. Dong. Oral administration of allergen extracts from Dermatophagoides farinae desensitizes specific allergen-induced inflammation and airway hyperresponsiveness in rats. Int Immunopharmacol. 2008; 8(12): 1639-1645.
• 28. Choi, Y.H., Yan, G.H., Chai, O.H., Lim, J.M., Sung, S.Y., Zhang, X., Kim, J.H., Choi, S.H., Lee, M.S., Han, E.H., Kim, H.T., Song, C.H. Inhibition of anaphylaxis-like reaction and mast cell activation by water extract from the fruiting body of Phellinus linteus.Biol Pharm Bull.; 2006; 29 (7):1360-1365
• 29. Moura CTM, Bezerra FC, Moraes IM, Magalh~aes PJC, Capaz FR. Increase responsiveness to 5-hydroxytryptamine after antigenic challenge is inhibited by nifedipine and niflumic acid in rat trachea in vitro. Clin Exp Pharmacol Physiol. 2005; 32:1119–1123.
• 30. Yang EJ, Lee J-S, Song BB, Yun C-Y, Kim D-H, Kim IS. Antiinflammatory effects of ethanolic extract from Lagerstroemia indica on airway inflammation in mice. J Ethnopharmacol. 2011; 136:422–427.
• 31. Hould R. 1984. Technical on histopathology and cytopathology. Maloine. 19-21:225–227.
• 32. Haines DM, Chelack BJ. Technical considerations for developing enzyme immunohistochemical staining procedures on formalinfixed paraffin-embedded tissues for diagnostic pathology. J Vet Diagn Invest 1991;3:101–112.
• 33. DiCosmo BF, Geba gp, Picarella D, Elias JA, Ranking JA, Stripp BR, Whetsett JA, Favell RA. Airway epithelial cell expression of interleukin-6 in transgenic mice. Uncooplingof airway inflammation and bronchial hyperreactivity. J Clin Invest. 1994; 94:2028–2035. [PubMed: 7962549]
• 34. Wills-Karp M, Gavett SH, Schofield B, Finkelman F. Role of interleukin-4 in the development of allergic airway inflammation and airway hyperresponsiveness. Av Exp Med Biol. 1995; 409:343– 347.
• 35. Serap C M., Serdar K., Tolgahan A., Hüsnü A B., Gonca K., Gökçen Ö Ş., Tuncay Ş., Osman Y. The effectıveness of thymoquınone in experimental model of asthma and role of vascular endothelial growth factor. Smyrna Med.J. 2013; 3: 34- 39. https:// ISSN: 2146- 670X.
• 36. S. Kalemci , S. Cilaker Micili , T. Acar , T. Senol , N. Dirican, G. Omeroglu , A. Bagriyanik , G. Kamaci , O. Yilmaz. Effectiveness of thymoquinone in the treatment of experimental asthma. Clin Ter 2013; 164 (3):e????. doi: 10.7417/CT.2013.1559.
• 37. Das, A.M., R.J. Flower, P.G. Hellewell, M.M. Teixeira, M. Perretti, 1997. A novel murine model of allergic inflammation to study the effect of dexamethasone on eosinophil recruitment. Br J Pharmacol., 121: 97-104. de Matos, O.G., S.S. Amaral, P.E. Pereira da Silva, D.A. Perez, D.M. Alvarenga, A.V. Ferreira, J.
• 38. Abeer A.A. Salama., Hala F. Zaki., Siham M. El-Shenawy., ELDenshary E.S.M., Ismaiel ElKassaby Ismaiel and Nermeen M. Shaffie. Effects of Fish oil and Dexamethasone in ExperimentallyInduced Bronchial Asthma. Australian J. of Basic and Applied Sciences, 2012 6(13): 497-506. ISSN 1991-8178.
• 39. Corrigan, C. 2012. Mechanisms of asthma. Medicine., 40 (5): 223- 227.
• 40. Bergeron C, Boulet LP. Structural changes in airway diseases: characteristics, mechanisms, consequences, and pharmacologic modulation. Chest 2006;129:106887.
• 41. Li X, Wilson JW. Increased vascularity of the bronchial mucosa in mild asthma. Am J Respir Crit Care Med 1997;156:229-33.
• 42. Arm JP, Lee TH. The pathobiology of bronchial asthma. Adv Immunol 1992;51:323-82.
• 43. Chun G L., Holger L., Peter B., Robert J H., Svetlana C., Vineet B., Min J K., Lauren C., Yoon K K., Donald M M., and Jack A E. Vascular endothelial growth factor (VEGF) induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. Nat Med . 2004 October ; 10(10): 1095–1103. doi:10.1038/ nm1105.
• 44. Vrugt B, et al. Bronchial angiogenesis in severe glucocorticoiddependent asthma. Eur. Respir. J. 2000; 15:1014–1021. [PubMed