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Application of γ-Aminobutyric Acid Treatment Differently Affects Physicochemical Characteristics of Tomato Fruits during Post-harvest Storage

Yıl 2021, Cilt: 38 Sayı: 2, 101 - 109, 01.12.2021
https://doi.org/10.16882/hortis.997921

Öz

The quality of tomato fruit, from harvest to human consumption, requires a lengthy period for shipping, storing, and marketing. γ-aminobutyric acid (GABA) is a good candidate because it is a natural substance produced by plants to defend themselves against stress conditions. In this study, the effect of post-harvest GABA treatments at 0 (control), 5 mM and, 20 mM on the physical and biochemical properties and the polysaccharide content of tomatoes during 28 days of storage were investigated. Our results indicated that 5 mM of GABA treatment increased firmness and shelf-life by maintaining the integrity of fruits compared to control and 20 mM of GABA treated fruits. The fruits treated with 5 mM of GABA decreased the amount of WSP and the expression of cell wall related genes Pectate lyase (PL) and Polygalacturonase (PG). There was not a clear difference in colour index (CI) values among all treated groups at the end of post-harvest storage. Moreover, the tomato fruits treated with 5 mM GABA also showed somewhat less ethylene production, respiration rate and expression level of two ethylene synthesis genes ACS2 and ACS4 towards the end of storage. These results suggested that treatment with 5 mM GABA could be a beneficial strategy for maintaining the morphological and biochemical quality of tomato under post-harvest storage conditions.

Kaynakça

  • Aghdam, M.S., Kakavand, F., Rabiei, V., Nahandi, F.Z., & Razavi, F. (2019). γ-Aminobutyric acid and nitric oxide treatments preserve sensory and nutritional quality of cornelian cherry fruits during postharvest cold storage by delaying softening and enhancing phenols accumulation. Scientia Horticulturae, 246:812-817.
  • Antoniou, C., Chatzimichail, G., Xenofontos, R., Pavlou, J.J., Panagiotou, E., Christou, A., & Fotopoulos, V. (2017). Melatonin systemically ameliorates drought stress-induced damage in Medicago sativa plants by modulating nitro-oxidative homeostasis and proline metabolism. Journal of Pineal Research, 62(4).
  • Bai, J., Alleyne, V., Hagenmaier, R.D., Mattheis, J.P., & Baldwin, E.A. (2003). Formulation of zein coatings for apple (Malus domestica Borkh). Postharvest Biology and Technology, 28:259–268.
  • Barry, C.S., Llop-Tous, M.I., & Grierson, D. (2000). The regulation of 1-aminocyclopropane-1-carboxylic acid synthase gene expression during the transition from system-1 to system-2 ethylene synthesis in tomato. Plant Physiology, 123:979-986.
  • Brummel, D.A., & Harpster, M.H. (2001). Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants. Plant Molecular Biology, 47:311-339.
  • Candir, E., Candir, A., & Sen, F. (2017) Effects of aminoethoxyvinylglycine treatment by vacuum infiltration method on postharvest storage and shelf life of tomato fruit, Postharvest Biology and Technology, 125:13-25.
  • Chen, Y.H., Hung, Y.C., Chen, M.Y., Lin, M.S., & Lin, H.T. (2019). Enhanced storability of blueberries by acidic electrolyzed oxidizing water application may be mediated by regulating ROS metabolism. Food Chemistry, 270:229-235.
  • Decros, G., Baldet, P., Beauvoit, B., Stevens, R., Flandin, A., Colombié, S., Gibon, Y., & Pétriacq, P. (2019). Get the balance right: ROS homeostasis and redox signalling in fruit. Frontiers in Plant Science, 10:1091.
  • Deewatthanawong, R., Rowell, P., & Watkins, C.B. (2010). γ-Aminobutyric acid (GABA) metabolism in CO2 treated tomatoes. Postharvest Biology and Technology, 57:97-105.
  • Distefano, M., Arena, E., Mauro, R.P., Brighina, S., Leonardi, C., Fallico, B., & Giuffrida, F. (2020). Effects of genotype, storage temperature and time on quality and compositional traits of cherry tomato. Foods, 9:1729.
  • Filisetti-Cozzi, T.M.C.C., & Carpita, N.C. (1991). Measurement of uronic acids without interference from neutral sugars. Analytical Biochemistry, 197:157-162.
  • Foyer, C.H. (2018). Reactive oxygen species, oxidative signaling and the regulation of photosynthesis. Environmental and Experimental Botany, 154:134-142.
  • Gao, H., Zhang, Z., Lv, X. G., Cheng, N., Peng, B.Z., & Cao, W. (2016). Effect of 24-epibrassinolide on chilling injury of peach fruit in relation to phenolic and proline metabolisms. Postharvest Biology and Technology, 111:390-397.
  • Giovannoni, J.J. (2007). Fruit ripening mutants yield insights into ripening control. Current Opinion in Plant Biology, 10:283–289.
  • Han, S., Nan, Y., Qu, W., He, Y., Ban, Q., Lv, Y., & Rao, J. (2018). Exogenous γ-Aminobutyric acid treatment that contributes to regulation of malate metabolism and ethylene synthesis in apple fruit during storage. Journal of Agricultural and Food Chemistry, 66:13473-13482.
  • Kinnersley, A.M., & Turano, F.J. (2000). γ-Aminobutyric acid (GABA) and plant responses to stress Critical Reviews in Plant Sciences, 19:479-509.
  • Klee, H.J., & Giovannoni, J.J. (2011). Genetics and control of tomato fruit ripening and quality attributes Annual Review of Genetics, 45:41-59.
  • Li, J., Zhou, X., Wei, B., Cheng, S., Zhou, Q., & Ji, S. (2019). GABA application improves the mitochondrial antioxidant system and reduces peel browning in ‘Nanguo’ pears after removal from cold storage. Food Chemistry, 297:124903.
  • Lin, Y., Lin, Y., Lin, H., Lin, M., Li, H., Yuan, F., Chen, Y., & Xiao, J. (2018) Effects of paper containing 1-MCP postharvest treatment on the disassembly of cell wall polysaccharides and softening in Younai plum fruit during storage. Food Chemistry, 264:1-8.
  • Lin, Y.X., Lin, H.T., Chen, Y.H., Wang, H., Lin, M.S., & Ritenour, M.A. (2020). The role of ROS-induced change of respiratory metabolism in pulp breakdown development of longan fruit during storage. Food Chemistry, 305:125439.
  • Livak, K.J., & Schmittgen, T.D. (2001). Analysis of relative gene expression data using real-time quantitative pcr and the 2 CT method. Methods, 25:402-408.
  • Lunn, D., Phan, T.D., Tucker, G.A., & Lycett, G.W. (2013). Cell wall composition of tomato fruit changes during development and inhibition of vesicle trafficking is associated with reduced pectin levels and reduced softening. Plant Physiology and Biochemistry, 66:91-97.
  • Makino, Y., Soga, N., Oshita, S., Kawagoe, Y., & Tanaka, A., (2008). Stimulation of γ-aminobutyric acid production in vine-ripe tomato (Lycopersicon esculentum Mill.) fruits under modified atmospheres. Journal of Agricultural and Food Chemistry, 56:7189–7193.
  • Malekzadeh, P., Khara, J., & Heydari, R. (2014) Alleviating effects of exogenous Gamma-aminobutiric acid on tomato seedling under chilling stress. Physiology and Molecular Biology of Plants, 20:133-137.
  • Mansourbahmani, S., Ghareyazie, B., Zarinnia, V., Kalatejari, S., & Mohammadi, R.Z. (2018). Study on the efficiency of ethylene scavengers on the maintenance of postharvest quality of tomato fruit. Journal of Food Measurement and Characterization, 12:691-701.
  • Mohammadrezakhani, S., Hajilou, J., Rezanejad, F., & Nahandi, F.Z. (2019). Assessment of exogenous application of proline on antioxidant compounds in three Citrus species under low temperature stress. Journal of Plant Interactions, 14:347-358.
  • Nangare, D.D., Singh, Y., Kumar, P.S., & Minhas, P.S., (2016). Growth, fruit yield and quality of tomato (Lycopersicon esculentum Mill.) as affected by deficit irrigation regulated on phenological basis. Agricultural Water Management, 171:73-79.
  • Nazoori, F., Zamani Bahramabadi, E., & Mirdehghan, S.H. (2020). Extending the shelf life of pomegranate (Punica granatum L.) by GABA coating application. Food Measure, 14: 2760-2772.
  • Niazi, Z., Razavi, F., Khademi, O., & Aghdam, M.S. (2021). Exogenous application of hydrogen sulfide and γ-aminobutyric acid alleviates chilling injury and preserves quality of persimmon fruit (Diospyros kaki, cv. Karaj) during cold storage. Scientia Horticulturae, 285:110198.
  • Park, M.H., Kim, S.J., Lee, J.S., Hong, Y.P., Chae, S.H., & Ku, K.M. (2021). Carbon dioxide pre-treatment and cold storage synergistically delay tomato ripening through transcriptional change in ethylene-related genes and respiration-related metabolism. Foods, 10:744.
  • Pokalsky, A.R., Hiatt, W.R., Ridge, N., Rasmussen, R., & Shewmaker, C. K. (1989) Structure and expression of elongation factor 1α in tomato. Nucleic Acids Research, 17:4666-4673.
  • Rastegar, S., Khankahdani, H.H., & Rahimzadeh, M. (2020). Effect of γ-aminobutyric acid on the antioxidant system and biochemical changes of mango fruit during storage. Food Measure, 14:778-789.
  • Seymour, G.B., Chapman, N.H., Chew, B.L., & Rose, J.K. C. (2013). Regulation of ripening and opportunities for control in tomato and other fruits. Plant Biotechnology Journal, 11:269-278.
  • Shang, H.T., Cao, S.F., Yang, Z.F., Cai, Y.T., & Zheng, Y.H. (2011). Effect of exogenous γ-aminobutyric acid treatment on proline accumulation and chilling injury in peach fruit after long-term cold storage. Journal of Agricultural and Food Chemistry, 59:264-1268.
  • Shekari, A., Hassani, R.N., & Aghdam, M.S. (2021). Exogenous application of GABA retards cap browning in Agaricus bisporus and its possible mechanism. Postharvest Biology and Technology, 174:111434.
  • Shelp, B.J., Bown, A.W., & McLean, M.D. (1999). Metabolism and functions of gamma-aminobutyric acid. Trends in Plant Sciences, 4:446-452.
  • Soleimani Aghdam, M., Naderi, R., Jannatizadeh, A., Sarcheshmeh, M.A., & Babalar, M. (2016) Enhancement of postharvest chilling tolerance of anthurium cut flowers by γ-aminobutyric acid (GABA) treatments. Scientia Horticulturae, 198:52-60.
  • Uluisik, S., Chapman, N.H., Smith, R., Poole, M., Adams, G., Gillis, R.B., Besong, T.M., Sheldon, J., Stiegelmeyer, S., Perez, L., Samsulrizal, N., Wang, D., Fisk, I.D., Yang, N., Baxter, C., Rickett, D., Fray, R., Blanco-Ulate, B., Powell, A.L., Harding, S.E., Craigon, J., Rose, J.K., Fich, E.A., Sun, L., Domozych, D.S., Fraser, P.D., Tucker, G.A., Grierson, D., & Seymour, G.B. (2016). Genetic improvement of tomato by targeted control of fruit softening. Nature Biotechnology, 34:950-2.
  • Wang, D., Yeats, T.H., Uluisik, S., Rose, J.K.C., & Seymour, G.B. (2018). Fruit Softening: Revisiting the Role of Pectin. Trends in Plant Science, 23:302-310. Wang, Y., Luo, Z., Mao, L., & Ying, T. (2016). Contribution of polyamines metabolism and GABA shunt to chilling tolerance induced by nitric oxide in cold-stored banana fruit. Food Chemistry, 197:333-339.
  • Wei, C., Ma, L., Cheng, Y., Guan, Y., & Guan, J. (2019). Exogenous ethylene alleviates chilling injury of ‘Huangguan’ pear by enhancing the proline content and antioxidant activity. Scientia Horticulturae, 257:208671.
  • Yan, M.A., Duan, S., & Zhao, M. (2016). Research progress of foods rich in gamma-aminobutyric acid. Biotic Resources, 38:1-6.
  • Zarei, L., Saba, M.K., & Vafaee, Y. (2020). Effect of gamma-amino-butyric acid (GABA) foliar application on chilling and postharvest quality of tomato (cv. Newton). Plant Productions, 43:199-212.
  • Zhang, X.H., Shen, L., Li, F.J., Zhang, Y.X., Meng, D.M., & Sheng, J.P. (2010). Up-regulating arginase contributes to amelioration of chilling stress and the antioxidant system in cherry tomato fruits. Journal of the Science of Food and Agriculture, 90:2195-2202.
  • Zhong, T.Y., Yao, G.F., & Wang, S.S. (2021). Hydrogen sulfide maintains good nutrition and delays postharvest senescence in postharvest tomato fruits by regulating antioxidative metabolism. Journal of Plant Growth Regulation. https://doi.org/10.1007/s00344-021-10377-4.
Yıl 2021, Cilt: 38 Sayı: 2, 101 - 109, 01.12.2021
https://doi.org/10.16882/hortis.997921

Öz

Kaynakça

  • Aghdam, M.S., Kakavand, F., Rabiei, V., Nahandi, F.Z., & Razavi, F. (2019). γ-Aminobutyric acid and nitric oxide treatments preserve sensory and nutritional quality of cornelian cherry fruits during postharvest cold storage by delaying softening and enhancing phenols accumulation. Scientia Horticulturae, 246:812-817.
  • Antoniou, C., Chatzimichail, G., Xenofontos, R., Pavlou, J.J., Panagiotou, E., Christou, A., & Fotopoulos, V. (2017). Melatonin systemically ameliorates drought stress-induced damage in Medicago sativa plants by modulating nitro-oxidative homeostasis and proline metabolism. Journal of Pineal Research, 62(4).
  • Bai, J., Alleyne, V., Hagenmaier, R.D., Mattheis, J.P., & Baldwin, E.A. (2003). Formulation of zein coatings for apple (Malus domestica Borkh). Postharvest Biology and Technology, 28:259–268.
  • Barry, C.S., Llop-Tous, M.I., & Grierson, D. (2000). The regulation of 1-aminocyclopropane-1-carboxylic acid synthase gene expression during the transition from system-1 to system-2 ethylene synthesis in tomato. Plant Physiology, 123:979-986.
  • Brummel, D.A., & Harpster, M.H. (2001). Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants. Plant Molecular Biology, 47:311-339.
  • Candir, E., Candir, A., & Sen, F. (2017) Effects of aminoethoxyvinylglycine treatment by vacuum infiltration method on postharvest storage and shelf life of tomato fruit, Postharvest Biology and Technology, 125:13-25.
  • Chen, Y.H., Hung, Y.C., Chen, M.Y., Lin, M.S., & Lin, H.T. (2019). Enhanced storability of blueberries by acidic electrolyzed oxidizing water application may be mediated by regulating ROS metabolism. Food Chemistry, 270:229-235.
  • Decros, G., Baldet, P., Beauvoit, B., Stevens, R., Flandin, A., Colombié, S., Gibon, Y., & Pétriacq, P. (2019). Get the balance right: ROS homeostasis and redox signalling in fruit. Frontiers in Plant Science, 10:1091.
  • Deewatthanawong, R., Rowell, P., & Watkins, C.B. (2010). γ-Aminobutyric acid (GABA) metabolism in CO2 treated tomatoes. Postharvest Biology and Technology, 57:97-105.
  • Distefano, M., Arena, E., Mauro, R.P., Brighina, S., Leonardi, C., Fallico, B., & Giuffrida, F. (2020). Effects of genotype, storage temperature and time on quality and compositional traits of cherry tomato. Foods, 9:1729.
  • Filisetti-Cozzi, T.M.C.C., & Carpita, N.C. (1991). Measurement of uronic acids without interference from neutral sugars. Analytical Biochemistry, 197:157-162.
  • Foyer, C.H. (2018). Reactive oxygen species, oxidative signaling and the regulation of photosynthesis. Environmental and Experimental Botany, 154:134-142.
  • Gao, H., Zhang, Z., Lv, X. G., Cheng, N., Peng, B.Z., & Cao, W. (2016). Effect of 24-epibrassinolide on chilling injury of peach fruit in relation to phenolic and proline metabolisms. Postharvest Biology and Technology, 111:390-397.
  • Giovannoni, J.J. (2007). Fruit ripening mutants yield insights into ripening control. Current Opinion in Plant Biology, 10:283–289.
  • Han, S., Nan, Y., Qu, W., He, Y., Ban, Q., Lv, Y., & Rao, J. (2018). Exogenous γ-Aminobutyric acid treatment that contributes to regulation of malate metabolism and ethylene synthesis in apple fruit during storage. Journal of Agricultural and Food Chemistry, 66:13473-13482.
  • Kinnersley, A.M., & Turano, F.J. (2000). γ-Aminobutyric acid (GABA) and plant responses to stress Critical Reviews in Plant Sciences, 19:479-509.
  • Klee, H.J., & Giovannoni, J.J. (2011). Genetics and control of tomato fruit ripening and quality attributes Annual Review of Genetics, 45:41-59.
  • Li, J., Zhou, X., Wei, B., Cheng, S., Zhou, Q., & Ji, S. (2019). GABA application improves the mitochondrial antioxidant system and reduces peel browning in ‘Nanguo’ pears after removal from cold storage. Food Chemistry, 297:124903.
  • Lin, Y., Lin, Y., Lin, H., Lin, M., Li, H., Yuan, F., Chen, Y., & Xiao, J. (2018) Effects of paper containing 1-MCP postharvest treatment on the disassembly of cell wall polysaccharides and softening in Younai plum fruit during storage. Food Chemistry, 264:1-8.
  • Lin, Y.X., Lin, H.T., Chen, Y.H., Wang, H., Lin, M.S., & Ritenour, M.A. (2020). The role of ROS-induced change of respiratory metabolism in pulp breakdown development of longan fruit during storage. Food Chemistry, 305:125439.
  • Livak, K.J., & Schmittgen, T.D. (2001). Analysis of relative gene expression data using real-time quantitative pcr and the 2 CT method. Methods, 25:402-408.
  • Lunn, D., Phan, T.D., Tucker, G.A., & Lycett, G.W. (2013). Cell wall composition of tomato fruit changes during development and inhibition of vesicle trafficking is associated with reduced pectin levels and reduced softening. Plant Physiology and Biochemistry, 66:91-97.
  • Makino, Y., Soga, N., Oshita, S., Kawagoe, Y., & Tanaka, A., (2008). Stimulation of γ-aminobutyric acid production in vine-ripe tomato (Lycopersicon esculentum Mill.) fruits under modified atmospheres. Journal of Agricultural and Food Chemistry, 56:7189–7193.
  • Malekzadeh, P., Khara, J., & Heydari, R. (2014) Alleviating effects of exogenous Gamma-aminobutiric acid on tomato seedling under chilling stress. Physiology and Molecular Biology of Plants, 20:133-137.
  • Mansourbahmani, S., Ghareyazie, B., Zarinnia, V., Kalatejari, S., & Mohammadi, R.Z. (2018). Study on the efficiency of ethylene scavengers on the maintenance of postharvest quality of tomato fruit. Journal of Food Measurement and Characterization, 12:691-701.
  • Mohammadrezakhani, S., Hajilou, J., Rezanejad, F., & Nahandi, F.Z. (2019). Assessment of exogenous application of proline on antioxidant compounds in three Citrus species under low temperature stress. Journal of Plant Interactions, 14:347-358.
  • Nangare, D.D., Singh, Y., Kumar, P.S., & Minhas, P.S., (2016). Growth, fruit yield and quality of tomato (Lycopersicon esculentum Mill.) as affected by deficit irrigation regulated on phenological basis. Agricultural Water Management, 171:73-79.
  • Nazoori, F., Zamani Bahramabadi, E., & Mirdehghan, S.H. (2020). Extending the shelf life of pomegranate (Punica granatum L.) by GABA coating application. Food Measure, 14: 2760-2772.
  • Niazi, Z., Razavi, F., Khademi, O., & Aghdam, M.S. (2021). Exogenous application of hydrogen sulfide and γ-aminobutyric acid alleviates chilling injury and preserves quality of persimmon fruit (Diospyros kaki, cv. Karaj) during cold storage. Scientia Horticulturae, 285:110198.
  • Park, M.H., Kim, S.J., Lee, J.S., Hong, Y.P., Chae, S.H., & Ku, K.M. (2021). Carbon dioxide pre-treatment and cold storage synergistically delay tomato ripening through transcriptional change in ethylene-related genes and respiration-related metabolism. Foods, 10:744.
  • Pokalsky, A.R., Hiatt, W.R., Ridge, N., Rasmussen, R., & Shewmaker, C. K. (1989) Structure and expression of elongation factor 1α in tomato. Nucleic Acids Research, 17:4666-4673.
  • Rastegar, S., Khankahdani, H.H., & Rahimzadeh, M. (2020). Effect of γ-aminobutyric acid on the antioxidant system and biochemical changes of mango fruit during storage. Food Measure, 14:778-789.
  • Seymour, G.B., Chapman, N.H., Chew, B.L., & Rose, J.K. C. (2013). Regulation of ripening and opportunities for control in tomato and other fruits. Plant Biotechnology Journal, 11:269-278.
  • Shang, H.T., Cao, S.F., Yang, Z.F., Cai, Y.T., & Zheng, Y.H. (2011). Effect of exogenous γ-aminobutyric acid treatment on proline accumulation and chilling injury in peach fruit after long-term cold storage. Journal of Agricultural and Food Chemistry, 59:264-1268.
  • Shekari, A., Hassani, R.N., & Aghdam, M.S. (2021). Exogenous application of GABA retards cap browning in Agaricus bisporus and its possible mechanism. Postharvest Biology and Technology, 174:111434.
  • Shelp, B.J., Bown, A.W., & McLean, M.D. (1999). Metabolism and functions of gamma-aminobutyric acid. Trends in Plant Sciences, 4:446-452.
  • Soleimani Aghdam, M., Naderi, R., Jannatizadeh, A., Sarcheshmeh, M.A., & Babalar, M. (2016) Enhancement of postharvest chilling tolerance of anthurium cut flowers by γ-aminobutyric acid (GABA) treatments. Scientia Horticulturae, 198:52-60.
  • Uluisik, S., Chapman, N.H., Smith, R., Poole, M., Adams, G., Gillis, R.B., Besong, T.M., Sheldon, J., Stiegelmeyer, S., Perez, L., Samsulrizal, N., Wang, D., Fisk, I.D., Yang, N., Baxter, C., Rickett, D., Fray, R., Blanco-Ulate, B., Powell, A.L., Harding, S.E., Craigon, J., Rose, J.K., Fich, E.A., Sun, L., Domozych, D.S., Fraser, P.D., Tucker, G.A., Grierson, D., & Seymour, G.B. (2016). Genetic improvement of tomato by targeted control of fruit softening. Nature Biotechnology, 34:950-2.
  • Wang, D., Yeats, T.H., Uluisik, S., Rose, J.K.C., & Seymour, G.B. (2018). Fruit Softening: Revisiting the Role of Pectin. Trends in Plant Science, 23:302-310. Wang, Y., Luo, Z., Mao, L., & Ying, T. (2016). Contribution of polyamines metabolism and GABA shunt to chilling tolerance induced by nitric oxide in cold-stored banana fruit. Food Chemistry, 197:333-339.
  • Wei, C., Ma, L., Cheng, Y., Guan, Y., & Guan, J. (2019). Exogenous ethylene alleviates chilling injury of ‘Huangguan’ pear by enhancing the proline content and antioxidant activity. Scientia Horticulturae, 257:208671.
  • Yan, M.A., Duan, S., & Zhao, M. (2016). Research progress of foods rich in gamma-aminobutyric acid. Biotic Resources, 38:1-6.
  • Zarei, L., Saba, M.K., & Vafaee, Y. (2020). Effect of gamma-amino-butyric acid (GABA) foliar application on chilling and postharvest quality of tomato (cv. Newton). Plant Productions, 43:199-212.
  • Zhang, X.H., Shen, L., Li, F.J., Zhang, Y.X., Meng, D.M., & Sheng, J.P. (2010). Up-regulating arginase contributes to amelioration of chilling stress and the antioxidant system in cherry tomato fruits. Journal of the Science of Food and Agriculture, 90:2195-2202.
  • Zhong, T.Y., Yao, G.F., & Wang, S.S. (2021). Hydrogen sulfide maintains good nutrition and delays postharvest senescence in postharvest tomato fruits by regulating antioxidative metabolism. Journal of Plant Growth Regulation. https://doi.org/10.1007/s00344-021-10377-4.
Toplam 44 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Ziraat Mühendisliği
Bölüm Araştırma Makalesi
Yazarlar

Selman Uluışık Bu kişi benim 0000-0003-0790-6705

Yayımlanma Tarihi 1 Aralık 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 38 Sayı: 2

Kaynak Göster

APA Uluışık, S. (2021). Application of γ-Aminobutyric Acid Treatment Differently Affects Physicochemical Characteristics of Tomato Fruits during Post-harvest Storage. Horticultural Studies, 38(2), 101-109. https://doi.org/10.16882/hortis.997921
AMA Uluışık S. Application of γ-Aminobutyric Acid Treatment Differently Affects Physicochemical Characteristics of Tomato Fruits during Post-harvest Storage. HortiS. Aralık 2021;38(2):101-109. doi:10.16882/hortis.997921
Chicago Uluışık, Selman. “Application of γ-Aminobutyric Acid Treatment Differently Affects Physicochemical Characteristics of Tomato Fruits During Post-Harvest Storage”. Horticultural Studies 38, sy. 2 (Aralık 2021): 101-9. https://doi.org/10.16882/hortis.997921.
EndNote Uluışık S (01 Aralık 2021) Application of γ-Aminobutyric Acid Treatment Differently Affects Physicochemical Characteristics of Tomato Fruits during Post-harvest Storage. Horticultural Studies 38 2 101–109.
IEEE S. Uluışık, “Application of γ-Aminobutyric Acid Treatment Differently Affects Physicochemical Characteristics of Tomato Fruits during Post-harvest Storage”, HortiS, c. 38, sy. 2, ss. 101–109, 2021, doi: 10.16882/hortis.997921.
ISNAD Uluışık, Selman. “Application of γ-Aminobutyric Acid Treatment Differently Affects Physicochemical Characteristics of Tomato Fruits During Post-Harvest Storage”. Horticultural Studies 38/2 (Aralık 2021), 101-109. https://doi.org/10.16882/hortis.997921.
JAMA Uluışık S. Application of γ-Aminobutyric Acid Treatment Differently Affects Physicochemical Characteristics of Tomato Fruits during Post-harvest Storage. HortiS. 2021;38:101–109.
MLA Uluışık, Selman. “Application of γ-Aminobutyric Acid Treatment Differently Affects Physicochemical Characteristics of Tomato Fruits During Post-Harvest Storage”. Horticultural Studies, c. 38, sy. 2, 2021, ss. 101-9, doi:10.16882/hortis.997921.
Vancouver Uluışık S. Application of γ-Aminobutyric Acid Treatment Differently Affects Physicochemical Characteristics of Tomato Fruits during Post-harvest Storage. HortiS. 2021;38(2):101-9.