Daidzein alleviates isoproterenol-induced cardiac injury in rats via NF-kB pathway inhibition
Yıl 2024,
, 124 - 130, 01.06.2024
Vadivelan Ramachandran
,
Vikash Vaikunth
,
Tharani Mohanasundaram
,
Ruchı Tıwarı
,
Harshini Magesh
Öz
The current study set out to explore the anti-inflammatory effects of Daidzein (Dz) on the isoproterenol (Iso)-induced myocardial infarction (MI) in male Wistar rats. Iso(80 mg/kg) was injected subcutaneously into rats to cause MI for two days at intervals of 24 hours. ELISA kits were used to measure the amounts of TNF-α & IL-6 in cardiac tissues. The quantity of NF-kB in the cytosol and nuclei was measured using a western blot approach. H&E staining was used to identify histological cardiac changes. In rats with Iso-induced myocardial infarction, Dz therapy shrunk the size of the infarct and reduced histological anomalies in the myocardium. Dz significantly lowered cardiac pro-inflammatory cytokine levels and prevented NF-kB nuclear translocation in MI-damaged rats, substantially reversing Iso-induced damage. This research shows that daidzein treatment in vivo reduces Iso-induced myocardial damage by preventing NF-kB activation, which may therefore reduce the release of inflammatory cytokines.
Kaynakça
- 1. Moran AE, Forouzanfar MH, Roth GA, Mensah GA, Ezzati M,
Flaxman A, et al. The global burden of ischemic heart disease
in 1990 and 2010: The global burden of disease 2010 study.
Circulation. 2014;129:1493–501. https://doi.org/10.1161/
CIRCULATIONAHA.113.004046
- 2. Reichert K, Colantuono B, McCormack I, Rodrigues F, Pavlov
V, Abid MR. Murine left anterior descending (LAD) coronary
artery ligation: An improved and simplified model for myo-
cardial infarction. J Vis Exp. 2017;2(122):55353. https://doi.
org/10.3791/55353
- 3. Frieden TR, Cobb LK, Leidig RC, Mehta S, Kass D. Reducing
Premature Mortality from Cardiovascular and Other Non-
Communicable Diseases by One Third: Achieving Sustainable
Development Goal Indicator 3.4.1. Global Heart. 2020;15:50.
https://doi.org/10.5334/gh.531
- 4. Balwan Wahied Khawar, Kour S. Lifestyle Diseases: The
Link between Modern Lifestyle and Threat to Public Health. Saudi J Med Pharm Sci. 2021;7(4):179–84. https://doi.org/10.136348/sjmp.2021.v07i04.00X
- 5. Silveira Rossi JL, Barbalho SM, Reverete de Araujo R, Bechara MD, Sloan KP, Sloan LA. Metabolic syndrome and cardiovascular diseases: Going beyond traditional risk factors. Diabetes/Metabolism Research and Reviews. 2022;38:e3502. https://doi.org/10.1002/dmrr.3502
- 6. Rana A, Samtiya M, Dhewa T, Mishra V, Aluko RE. Health
benefits of polyphenols: A concise review. J of Food Biochem.
2022;46:e14264. https://doi.org/10.1111/jfbc.14264
- 7. Ku YS, Ng MS, Cheng SS, Luk CY, Ludidi N, Chung G, et
al. Soybean secondary metabolites and flavors: The art of
compromise among climate, natural enemies, and human culture. In: Advances in Botanical Research. 2022;102:295–347. https://doi.org/10.1016/bs.abr.2022.03001
- 8. Messina M. Soy and health update: Evaluation of the clinical and epidemiologic literature. Nutrients. 2016;8:754. https://doi.org/10.3390/nu8120754
- 9. Szeja W, Grynkiewicz G, Rusin A. Isoflavones, their Glycosides and Glycoconjugates. Synthesis and Biological Activity. Curr Org Chem. 2017;21:218–35. https://doi.org/10.2174/1385272820666160928120822
- 10. Qaseem A, Forciea MA, McLean RM, Denberg TD. Treatment of low bone density or osteoporosis to prevent fractures in men and women: A clinical practice guideline update from the American college of physicians. Annals of Int Med. 2017;166:818-839. https://doi.org/10.7326/M15-1361.
- 11. Song Z, Wang X, He L, Chen L, Ren Z, Song S. Suppression of lysosomal-associated protein transmembrane 5 ameliorates cardiac function and inflammatory response by inhibiting the nuclear factor-kappa B (NF-κB) pathway after myocardial infarction in mice. Exp Anim. 2022;71(4):415–25.
- 12. Khalil MI, Ahmmed I, Ahmed R, Tanvir EM, Afroz R, Paul S, et al. Amelioration of Isoproterenol-Induced Oxidative Damage in Rat Myocardium by Withania somnifera Leaf Extract. Biomed Res Int. 2015;2015:1-10. https://doi.org/10.1155/2015/624159
- 13. Ma M, Uekawa K, Hasegawa Y, Nakagawa T, Katayama T, Sueta D, et al. Pretreatment with rosuvastatin protects against focal cerebral ischemia/reperfusion injury in rats through attenuation of oxidative stress and inflammation. Brain Res. 2013;26:87–94. https://doi.org/10.1016/j.brain-res.2013.04.040
- 14. Kumar M, Kasala ER, Bodduluru LN, Dahiya V, Sharma
D, Kumar V, et al. Animal models of myocardial infarction:
Mainstay in clinical translation. Regul Toxicol Pharmacol.
2016;76:221–30. https://doi.org/10.1016/j.yrtph.201603.005
- 15. Frangogiannis NG, Smith CW, Entman ML. The inflammatory response in myocardial infarction. Cardiovascular Research. 2002;53:31–47. https://doi.org/10.1016/S00086363(01)00434-5
- 16. Amran AZ, Jantan I, Dianita R, Buang F. Protective effects of
the standardized extract of Zingiber officinale on myocardium
against isoproterenol-induced biochemical and histopathological alterations in rats. Pharm Biol. 2015;53(12):1795–802. https://doi.org/10.3109/13880209.2015.1008147
- 17. Abdalla Hussein M. Cardioprotective Effects of Astaxanthin against Isoproterenol-Induced Cardiotoxicity in Rats. J Nutr Food Sci. 2014;5:1000335 https://doi.org/10.4172/2155-9600
- 18. Barnaba C, Medina-Meza IG. Flavonoids Ability to Disrupt Inflammation Mediated by Lipid and Cholesterol Oxidation. In: Advances in Experimental Medicine and Biology. 2019;1161:243–53. https://doi.org/10.1007/978-3-030-21735-8_19
Daidzein alleviates isoproterenol-induced cardiac injury in rats via NF-kB pathway inhibition
Yıl 2024,
, 124 - 130, 01.06.2024
Vadivelan Ramachandran
,
Vikash Vaikunth
,
Tharani Mohanasundaram
,
Ruchı Tıwarı
,
Harshini Magesh
Öz
The current study set out to explore the anti-inflammatory effects of Daidzein (Dz) on the isoproterenol (Iso)-induced myocardial infarction (MI) in male Wistar rats. Iso(80 mg/kg) was injected subcutaneously into rats to cause MI for two days at intervals of 24 hours. ELISA kits were used to measure the amounts of TNF-α & IL-6 in cardiac tissues. The quantity of NF-kB in the cytosol and nuclei was measured using a western blot approach. H&E staining was used to identify histological cardiac changes. In rats with Iso-induced myocardial infarction, Dz therapy shrunk the size of the infarct and reduced histological anomalies in the myocardium. Dz significantly lowered cardiac pro-inflammatory cytokine levels and prevented NF-kB nuclear translocation in MI-damaged rats, substantially reversing Iso-induced damage. This research shows that daidzein treatment in vivo reduces Iso-induced myocardial damage by preventing NF-kB activation, which may therefore reduce the release of inflammatory cytokines.
Teşekkür
We thank the Department of Pharmacology (DST-FIST, Sponsored) at JSS College of Pharmacy, Ooty, The Nilgiris, Tamil Nadu, India, for their kind assistance and infrastructure to carryout the research work.
Kaynakça
- 1. Moran AE, Forouzanfar MH, Roth GA, Mensah GA, Ezzati M,
Flaxman A, et al. The global burden of ischemic heart disease
in 1990 and 2010: The global burden of disease 2010 study.
Circulation. 2014;129:1493–501. https://doi.org/10.1161/
CIRCULATIONAHA.113.004046
- 2. Reichert K, Colantuono B, McCormack I, Rodrigues F, Pavlov
V, Abid MR. Murine left anterior descending (LAD) coronary
artery ligation: An improved and simplified model for myo-
cardial infarction. J Vis Exp. 2017;2(122):55353. https://doi.
org/10.3791/55353
- 3. Frieden TR, Cobb LK, Leidig RC, Mehta S, Kass D. Reducing
Premature Mortality from Cardiovascular and Other Non-
Communicable Diseases by One Third: Achieving Sustainable
Development Goal Indicator 3.4.1. Global Heart. 2020;15:50.
https://doi.org/10.5334/gh.531
- 4. Balwan Wahied Khawar, Kour S. Lifestyle Diseases: The
Link between Modern Lifestyle and Threat to Public Health. Saudi J Med Pharm Sci. 2021;7(4):179–84. https://doi.org/10.136348/sjmp.2021.v07i04.00X
- 5. Silveira Rossi JL, Barbalho SM, Reverete de Araujo R, Bechara MD, Sloan KP, Sloan LA. Metabolic syndrome and cardiovascular diseases: Going beyond traditional risk factors. Diabetes/Metabolism Research and Reviews. 2022;38:e3502. https://doi.org/10.1002/dmrr.3502
- 6. Rana A, Samtiya M, Dhewa T, Mishra V, Aluko RE. Health
benefits of polyphenols: A concise review. J of Food Biochem.
2022;46:e14264. https://doi.org/10.1111/jfbc.14264
- 7. Ku YS, Ng MS, Cheng SS, Luk CY, Ludidi N, Chung G, et
al. Soybean secondary metabolites and flavors: The art of
compromise among climate, natural enemies, and human culture. In: Advances in Botanical Research. 2022;102:295–347. https://doi.org/10.1016/bs.abr.2022.03001
- 8. Messina M. Soy and health update: Evaluation of the clinical and epidemiologic literature. Nutrients. 2016;8:754. https://doi.org/10.3390/nu8120754
- 9. Szeja W, Grynkiewicz G, Rusin A. Isoflavones, their Glycosides and Glycoconjugates. Synthesis and Biological Activity. Curr Org Chem. 2017;21:218–35. https://doi.org/10.2174/1385272820666160928120822
- 10. Qaseem A, Forciea MA, McLean RM, Denberg TD. Treatment of low bone density or osteoporosis to prevent fractures in men and women: A clinical practice guideline update from the American college of physicians. Annals of Int Med. 2017;166:818-839. https://doi.org/10.7326/M15-1361.
- 11. Song Z, Wang X, He L, Chen L, Ren Z, Song S. Suppression of lysosomal-associated protein transmembrane 5 ameliorates cardiac function and inflammatory response by inhibiting the nuclear factor-kappa B (NF-κB) pathway after myocardial infarction in mice. Exp Anim. 2022;71(4):415–25.
- 12. Khalil MI, Ahmmed I, Ahmed R, Tanvir EM, Afroz R, Paul S, et al. Amelioration of Isoproterenol-Induced Oxidative Damage in Rat Myocardium by Withania somnifera Leaf Extract. Biomed Res Int. 2015;2015:1-10. https://doi.org/10.1155/2015/624159
- 13. Ma M, Uekawa K, Hasegawa Y, Nakagawa T, Katayama T, Sueta D, et al. Pretreatment with rosuvastatin protects against focal cerebral ischemia/reperfusion injury in rats through attenuation of oxidative stress and inflammation. Brain Res. 2013;26:87–94. https://doi.org/10.1016/j.brain-res.2013.04.040
- 14. Kumar M, Kasala ER, Bodduluru LN, Dahiya V, Sharma
D, Kumar V, et al. Animal models of myocardial infarction:
Mainstay in clinical translation. Regul Toxicol Pharmacol.
2016;76:221–30. https://doi.org/10.1016/j.yrtph.201603.005
- 15. Frangogiannis NG, Smith CW, Entman ML. The inflammatory response in myocardial infarction. Cardiovascular Research. 2002;53:31–47. https://doi.org/10.1016/S00086363(01)00434-5
- 16. Amran AZ, Jantan I, Dianita R, Buang F. Protective effects of
the standardized extract of Zingiber officinale on myocardium
against isoproterenol-induced biochemical and histopathological alterations in rats. Pharm Biol. 2015;53(12):1795–802. https://doi.org/10.3109/13880209.2015.1008147
- 17. Abdalla Hussein M. Cardioprotective Effects of Astaxanthin against Isoproterenol-Induced Cardiotoxicity in Rats. J Nutr Food Sci. 2014;5:1000335 https://doi.org/10.4172/2155-9600
- 18. Barnaba C, Medina-Meza IG. Flavonoids Ability to Disrupt Inflammation Mediated by Lipid and Cholesterol Oxidation. In: Advances in Experimental Medicine and Biology. 2019;1161:243–53. https://doi.org/10.1007/978-3-030-21735-8_19