Simultaneous Determination of Ascorbic Acid, Paracetamol, Aspirin in Tablets Using UPLC

Year 2016, Issue: 2, 135 - 149, 01.06.2016

Faysal Selimoğlu Yücel Kadıoğlu Erdal Dinç

Abstract

Ultra-high-performance liquid chromatographic UPLC data obtained from photodiode array PDA detection was processed by the PCR and PLS algorithms for the simultaneous quantitative resolution of Ascorbic Acid AA , Paracetamol PAR , Aspirin ASP in a commercial formulation. Principle component regression PCR and partial least squares PLS were applied to the peak area ratio of the analytes/internal standard at multi-wavelength PDA detector responses. The combined use of UPLC and chemometric calibration techniques was denoted UPLC- PCR and UPLC-PLS. For the comparison purpose, the UPLC method was used for the confirmation of the results obtained from combined UPLC-chemometric calibration techniques. A good chromatographic separation between drugs and internal standard IS was achieved using a Waters ACQUITY UPLC BEH Phenyl 100 mm x 1.0 mm, i.d., 1.7 μm column and a mobile phase consisting of 0.1 M CH3COOH and methanol 75:25, v/v . The multi-wavelength PDA detection for Ascorbic Acid AA , Paracetamol PAR , Aspirin ASP was accomplished by measuring the peak area at the wavelength set corresponding to 245, 250, 255.0, 260.0, 265, 270, 275 and 280 nm. The proposed UPLC-PCR and UPLC-PLS approaches were validated by using the synthetic mixtures, and inter-day and intra-day experiments. The proposed methods were successfully applied to commercial samples containing the analyzed drugs.

Keywords

Quantitative resolution, UPLC method, Paracetamol, Ascorbic acid, Aspirin, Pharmaceutical preparation

Tabletlerde Askorbik Asit, Parasetamol ve Aspirinin UPLC Yöntemi ile Eşzamanlı Analizi

Abstract

Ticari tablet formülasyonu içerisindeki Askorbik Asit AA , Parasetamol PAR ve Aspirinin ASP ’nin kantitatif eş zamanlı miktar tayinleri için fotodiod dizili dedeksiyonu PDA kullanılarak elde edilen aşırı yüksek perfomranslı sıvı kromatografik UPLC verileri, kısmi en küçük kareler PLS ve temel bileşen regresyon PCR algoritmaları ile proses edildi. Kısmi en küçük kareler ve temel bileşen regresyon algoritmaları, çok dalgaboyunda PDA detektör cevaplarından elde edilen analit/iç standart pik alanları oranına uygulandı. UPLC ve kemometrik kalibrasyon tekniklerinin kombine kullanımı, UPLC-PCR ve UPLC-PLS olarak adlandırıldı. Karşılaştırma amacıyla, klasik UPLC yöntemi, kombine UPLC kemometrik tekniklerden elde edilen sonuçları doğrulamak için kullanıldı. Analiz edilen ilaçlar ve iç standart madde arasında iyi bir kromatografik ayrımı, Waters ACQUITY UPLC BEH fenil 100 mm x 1.0 mm, i.d., 1.7 μm kolon ile 0.1 M CH3COOH ve metanol 75:25, v/v kullanılarak gerçekleşitrildi. Çalışmada Askorbik Asit AA , Parasetamol PAR ve Aspirinin ASP ’nin çoklu dalga boylarındaki PDA ölçümleri 245, 250, 255.0, 260.0, 265, 270, 275 ve 280 nm dalgaboylarına karşılık gelen dalga boylarının pik alanlarının ölçümü ile elde edildi. KAF’ın internal standart olarak varlığında, Askorbik Asit AA , Parasetamol PAR ve Aspirinin ASP ’nin sentetik karışımları ile gün içi ve günler arası numunelerin anal izleriyle UPLC-PCR ve UPLC-PLC yöntemlerinin validasyonu gerçekleştirildi. Bu çalışmada önerilen yöntemlerin analizi edilen ilaçları içeren ticari örnekleri analizlerine başarıyla uygulandı.

Keywords

Kantitatif çözümleme, UPLC metodu, Parasetamol, Askorbik asid, Aspirin, Pharmasötik Hazırlık

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