Dicle Nehrindeki Balık ve Su Örneklerinde Vibrio parahaemolyticus’un PCR Yöntemi İle İdentifikasyonu

Abstract

İklim değişikliğinin bir sonucu olarak artan sayıda Vibrio parahaemolyticus enfeksiyonu tespit edilmiştir. Su sıcaklığı çok güçlü bir ekolojik faktördür. Dolayısıyla su ortamlarında ve su ürünlerinde V. parahaemolyticus'u takip etmek önem arzetmektedir. Bu çalışmanın amacı, Dicle nehrinde bulunan Malabadi Köprüsü, Fiskaya, Ongözlü Köprüsü, Bağıvar, Pamukçay, Ambarçay, Seyrantepe ve Silvan olmak üzere sekiz farklı yerdeki Vibrio parahaemolyticus'un varlığını araştırmaktır. Bu amaçla toplam 480 su ve balık numunesi kullanılmıştır. Bakteri izolasyonu için örnekler Tiyosülfatsitratsafra tuzu sukroz (TCBS) Agara ekim yapılmış, 37°C'de 24 saat inkübe edilmiştir ve bakteri kolonileri, TCBS agar plakalarına üç kez sürme yoluyla saflaştırılmıştır. Patojenin kesin tespiti PCR testi ile gerçekleştirilmiştir. Araştırmanın sonucunda Seyrantepe'deki 40 (6.6%) adet balık örneğinde patojenin varlığı tespit edilmiştir.

Keywords

• vibrio parahaeomolyticus
• Balık
• PCR
• Su

Identification of Vibrio parahaemolyticus by PCR Method in Fish and Water Samples from Tigris River, Turkey

Abstract

Climate change has caused increasing Vibrio parahaemolyticus infections in areas unaffected by this pathogen. Water temperature is an important ecological factor. It is consequently of high importance to follow V. parahaemolyticus in aquatic environments and foods. The aim of this work was to detect the presence of V. parahaemolyticus in Tigris River from eight different sites namely Malabadi Bridge, Fiskaya, Ongözlü Bridge, Bağıvar, Pamukçay, Ambarçay, Seyrantepe and Silvan. Total 480 samples of water and fish were used for V. parahaemolyticus detection. Thiosulphatecitrate‐bile salt sucrose (TCBS) Agar was employed for bacteria isolation for 24 h at 37◦C. The dominant uniform bacterial colonies were purified by streaking onto the TCBS agar plates three times. Identification of this pathogen was made by PCR. A total of 480 water and fish samples were analyzed. According to the results, V. parahaemolyticus was detected in 40 (6.6%) fish samples taken from the Seyrantepe region.

Keywords

• pcr
• Vibrio parahaemolyticus
• Fish
• Water

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