Localization of Desmin, Vimentin, and Laminin in the Syrinx of the Chukar Partridge (Alectoris chukar)

Abstract

The importance of vocalizations in the lives of birds has attracted the attention of many researchers to date. Extensive studies on this subject suggest that the male's song has essential effects on the female's decision-making, that it may provide information about the male's possible hormonal status and physical strength, and that this may be effective in deciding the female's choice of mate. Although they have a larynx like mammals, birds use the syrinx, localized deep in the chest between the trachea and lungs, to sing. Our study aimed to investigate the possible effects of desmin, vimentin, and laminin on the syrinx. In this context, epithelial tissue, muscle tissue, cartilage tissue, and glands of the syrinx were examined, and the presence of these filaments was histologically investigated using immunohistochemical methods. It was determined that desmin, vimentin, and laminin did not vary between male and female partridges. Desmin and vimentin showed a moderate-intensity reaction in smooth muscle cells. Laminin showed strong reactions in muscles; it also showed strong reactions in glands, epithelial cells, and vessel walls. Based on these findings, we can say that these intermediary filaments participate in the cytoskeletal structure of cell groups in the partridge syrinx, maintaining homeostasis in the cells and the physiological processes by which the organ functions.

Keywords

• Desmin
• Keklik
• Laminin
• Sirinks

Kınalı Keklik (Alectoris chukar) Sirinks’inde Desmin, Vimentin ve Laminin Lokalizasyonu

Abstract

Kuşlarda sesin yaşamlarındaki önemi günümüze kadar birçok araştırmacının ilgisini çekmiştir. Bununla ilgili yapılan geniş çaplı çalışmalar erkeğin ötüşünün dişinin karar kılmasında önemli etkileri olduğunu, erkeğin muhtemel hormonal durumu ve fiziksel gücü hakkında bilgi veriyor olabileceğini, bu durumun dişinin eş seçiminde karar kılmada etkili olabileceğini düşündürmektedir. Memelilerdeki gibi larinks bulunmasına karşın kuşlar şarkılarını söylemek için göğüslerinin derinliklerine yerleşmiş trakea ve akciğerler arasında lokalize olan sirinksi kullanmaktadırlar. Çalışmamızın amacı, desmin, vimentin ve laminin’in sirinks üzerine olası etkilerini araştırmaktır. Bu kapsamda sirinks’in epitel dokusu, kas dokusu, kıkırdak dokusu ve bezleri incelenmiş ve bu filamanların varlıkları histolojik olarak immünohistokimyasal yöntemler kullanılarak araştırılmıştır. Yapılan incelemeler sonucu desmin, vimentin ve laminin’in erkek ve dişi kınalı keklikler arasında değişkenlik göstermediği tespit edildi. Desmin ve vimentin’in düz kas hücrelerinde orta yoğunluklu bir reaksiyon gösterdiği görüldü. Laminin’in kaslarda güçlü reaksiyonlar gösterdiği; bezlerde, epitelyum hücrelerinde ve damar duvarlarında da güçlü reaksiyonlar gösterdiği görüldü. Elde edilen bu verilerden yola çıkarak bu intermediyer filamanların, keklik sirinksindeki hücre gruplarının iskelet yapısına katılarak hücrelerdeki homeostazının sağlanmasında ve organın fonksiyon gördüğü fizyolojik süreçlerin devamlılığında etkileri olduğunu söyleyebiliriz.

Keywords

• Desmin
• Keklik
• Laminin
• Sirinks

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