The Effects of Regular Treadmill Exercise on Some Blood Parameters in Diabetes Mellitus

Abstract

This study aims to determine the effects of regular treadmill exercise on some blood parameters in rats with an experimental diabetes model. In the study, 36 Wistar Albino rats were divided into four groups Control (C), Exercise (E), Diabetes (D), and Diabetes+Exercise (DE). A single dose of 60 mg/kg STZ solution was injected intraperitoneally (i.p) into the D and DE groups to create a diabetes model. After diabetes induction, E and DE groups were given running exercises at 20 m/min and 45 minutes daily for four weeks. According to the data obtained, while lipid profile (TC, LDL, TG) and liver enzyme (ALT, AST) levels increased significantly in group D compared to group C, HDL and TP levels were found to decrease. However, a decrease was observed in parameters other than HDL and TP in the DE group. It was also observed that the mineral and vitamin (Vit D, Ca, Mg, and Zn) levels decreased in the D group compared to the C group, while these values tended to increase in the DE group. As a result, the experiment shows that a four week regular exercise program in rats with diabetes has a therapeutic, preventive, and protective effect on lipoprotein levels, liver enzyme markers, and micronutrient levels.

Keywords

• Diabetes Mellitus
• Exercise
• Lipid profile
• Liver enzymes
• Micronutrients

Diabetes Mellitus'ta Düzenli Koşu Bandı Egzersizinin Bazı Kan Parametreleri Üzerindeki Etkisi

Abstract

Bu çalışma, deneysel diyabet modeli oluşturulan sıçanlarda düzenli koşu bandı egzersizinin bazı kan parametreleri üzerindeki etkilerini belirlemeyi amaçlamaktadır. Çalışmada 36 adet Wistar Albino rat Kontrol (K), Egzersiz (E), Diyabet (D) ve Diyabet+Egzersiz (DE) olmak üzere dört gruba ayrıldı. D ve DE gruplarına diyabet modeli oluşturmak için tek doz 60 mg/kg STZ solüsyonu intraperitoneal (i.p) olarak enjekte edildi. Diyabet indüksiyonu sonrası, E ve DE gruplarına dört hafta boyunca, 20 m/dk hızda ve günlük 45 dk. koşu egzersizi yaptırıldı. Elde edilen verilere göre, K grubuna göre D grubunda lipid profil (TK, LDL, TG) seviyeleri ile karaciğer enzim (ALT, AST) düzeyleri önemli ölçüde artış gösterirken, HDL ve TP düzeylerinin azaldığı tespit edildi. Ancak DE grubunda HDL ve TP dışındaki parametrelerde bir düşüş gözlendi. Yine K grubuna oranla D grubunda mineral ve vitamin (Vit D, kalsiyum, magnezyum ve çinko) seviyelerinin azaldığı gözlenirken, DE grubunda bu değerlerin artma eğiliminde olduğu belirlendi. Sonuç olarak, deneysel diyabetli sıçanlarda dört haftalık düzenli egzersiz programının lipoprotein seviyeleri, karaciğer enzim belirteçleri ve mikro besin seviyeleri üzerinde terapötik, önleyici ve koruyucu bir etkiye sahip olduğunu göstermektedir.

Keywords

• diabetes mellitus
• egzersiz
• lipid profili
• karaciğer enzimleri
• mikrobesinler

References

1. Abdella Baragob AE, AlMalki WH, Farag Alla HEH, Ibrahim A, Muhammed SK, & Abdella S, 2014: Investigate evaluation of oxidative stress and lipid profile in STZ-induced rats treated with antioxidant vitamin. Pharm Pharmacol, 5 (03), 272-279.
2. Ahangarpour A, Shabani R, & Farbood Y, 2018: The effect of betulinic acid on leptin, adiponectin, hepatic enzyme levels and lipid profiles in streptozotocin–nicotinamide-induced diabetic mice. Res Pharm Sci, 13 (2), 142.
3. Ahmadizad S, & El-Sayed MS, 2005: The acute effects of resistance exercise on the main determinants of blood rheology. J Sports Sci, 23 (3), 243-249.
4. Akbari A, Tadibi V, & Behpoor N, 2020: Effect of Aerobic Exercise Combined With Stevia Extract Supplementation on Selected Liver Enzymes in Diabetic Rats. CMJA, 10 (3), 244-257.
5. Aly YE, Abdou AS, Rashad MM, & Nassef MM, 2016: Effect of exercise on serum vitamin D and tissue vitamin D receptors in experimentally induced type 2. Diabetes Mellitus. J Adv Res, 7 (5), 671-679.
6. Amaechi D, Udosen E, Edet E, Asanga E, & Mbakwe I, 2015: Lipid profile status of streptozotocin induced diabetic rats treated with ethanolic leaf extract of Solenostemon monostachyus. J Med Plant Res, 9 (8), 289-293.
7. Analike RA, Ihim A, Iweanya S, Ogbodo E, Onah C, Asomugha A, Amah A, Obi P, Nwobodo E, & Timothy C, 2019: Assessment of glycated haemoglobin, total protein and albumin levels in patients with type 2 diabetes mellitus visiting NAUTH, Nnewi. Indian J Pathol Oncol, 6 (4), 700-703.
8. Balducci S, Sacchetti M, Haxhi J, Orlando G, D'Errico V, Fallucca S, Menini S, & Pugliese G, 2014: Physical exercise as therapy for type 2 diabetes mellitus. Diabetes Metab Res Rev, 30 (S1), 13-23.

9. Barbagallo M, & Dominguez LJ 2015: Magnesium and type 2 diabetes. World J Diabetes, 6 (10), 1152.
10. Baydil B, 2013: Serum macro-micro element responses to acute maximal physical exercise. World Appl Sci J, 23 (7), 945-949.
11. Bicer M, Akil M, Sivrikaya A, Kara E, Baltaci AK, & Mogulkoc R, 2011: Effect of zinc supplementation on the distribution of various elements in the serum of diabetic rats subjected to an acute swimming exercise. J Physiol Biochem, 67, 511-517.
12. Butola LK, Gusain N, Kute PK, Anjankar A, & Vagga AA, 2020: To study the association of vitamin D, calcium and phosphorus in type 2 diabetes mellitus patients. Age, 53 (11.2), 52-56.
13. Bürger-Mendonça M, Bielavsky M, & Barbosa FC, 2008: Liver overload in Brazilian triathletes after half-ironman competition is related muscle fatigue. Ann Hepatol, 7 (3), 245-248.
14. da Silva Bandeira V, Pires LV, Hashimoto LL, de Alencar LL, Almondes KGS, Lottenberg SA, & Cozzolino SMF, 2017: Association of reduced zinc status with poor glycemic control in individuals with type 2 diabetes mellitus. J Trace Elem Med Biol, 44, 132-136.
15. Dhavane S, & Dhavane P, 2022: Serum magnesium level in type 2 Diabetes mellitus and its relationship with glycemic control and diabetic complications. EJMCM, 9 (08), 2022.
16. Dönmez N, Keskin E, Özsan M, Shoshın OMA, & Mehtap B, 2020: The Favorable Effect of Swimming on Some Blood Parameters in Diabetic Rats. Ataturk Univ Vet Bilim Derg, 15 (1), 31-37.
17. Duzguner V, & Kaya S, 2007: Effect of zinc on the lipid peroxidation and the antioxidant defense systems of the alloxan-induced diabetic rabbits. Free Radic Biol Med, 42 (10), 1481-1486.
18. Ekperikpe US, Owolabi OJ, & Olapeju BI, 2019: Effects of Parkia biglobosa aqueous seed extract on some biochemical, haematological and histopathological parameters in streptozotocin induced diabetic rats. J Ethnopharmacol, 228, 1-10.
19. Eldin AB, Diab AA, Hasan MM, Mamoud N, & Zaitoun A, 2021: Effect of vitamin D supplementation and muscular exercise on irisin serum level and related metabolic parameters in type II diabetic albino rats. Gagandeep D, Shailaza S, & Rahul R, 2015: Evaluation of trace elements and glycated hemoglobin in type 2 diabetes mellitus. World J Pharm Pharm Sci, 4 (5), 940-947.
20. Gailiūnienė A, Stasiulis A, & Michailovienė J, 2007: The effect of submaximal exercise on blood creatinine, urea, total protein and uric acid levels of trained and untrained subjects. Ugdymas. Kūno kultūra Sportas, (3), 5-10.
21. Goboza M, Aboua YG, Chegou N, & Oguntibeju OO, 2019: Vindoline effectively ameliorated diabetes-induced hepatotoxicity by docking oxidative stress, inflammation and hypertriglyceridemia in type 2 diabetes-induced male Wistar rats. Biomed Pharmacother, 112, 108638.
22. Gómez T, Bequer L, Mollineda A, Molina JL, Álvarez A, Lavastida M, & Clapés S, 2017: Concentration of zinc, copper, iron, calcium, and magnesium in the serum, tissues, and urine of streptozotocin-induced mild diabetic rat model. Biol Trace Elem Res, 179, 237-246.
23. Gürbüz M, & Çelik MN, 2019: Mineraller ve Diabetes Mellitus. Turkiye Klinikleri J Intern Med, 4 (2), 71-83.
24. Hadi WH, Khudair TT, & Al-Fartosi KG, 2023: Level of lipid profile and liver enzyme of diabetic male rats induced by streptozotocin treated with forxiga. 3c Empresa: investigación y pensamiento crítico, 12 (1), 273-288.
25. Hatipoğlu D, Kahraman O, 2021: Hypoglycemic shock and acute liver injury in a dog associated with xylitol toxicity. J VetBio Sci Tech, 6 (2), 165-170.
26. Huskisson E, Maggini S, & Ruf M, 2007: The role of vitamins and minerals in energy metabolism and well-being. J Int Med Res, 35 (3), 277-289.
27. Jialal I, & Singh G, 2019: Management of diabetic dyslipidemia: an update. World J Diabetes, 10 (5), 280.
28. Kalas MA, Chavez L, Leon M, Taweesedt PT, & Surani S, 2021: Abnormal liver enzymes: A review for clinicians. World J Hepatol, 13 (11), 1688.
29. Kathak RR, Sumon AH, Molla NH, Hasan M, Miah R, Tuba HR, Habib A, & Ali N, 2022: The association between elevated lipid profile and liver enzymes: a study on Bangladeshi adults. Sci Rep, 12 (1), 1-8.
30. Kaynar Ö, Öztürk N, Kıyıcı F, Baygutalp N, & Bakan E, 2016: The effects of short-term intensive exercise on levels of liver enzymes and serum lipids in kick boxing athletes. Dicle Tip Derg, 43 (1), 130-134.
31. Khan AR, & Awan FR, 2014: Metals in the pathogenesis of type 2 diabetes. JDMDC, 13, 1-6.
32. Khaoshbaten M, Gholami N, Sokhtehzari S, Monazami AH, & Nejad MR, 2013: The effect of an aerobic exercise on serum level of liver enzymes and liver echogenicity in patients with non alcoholic fatty liver disease. Gastroenterol Hepatol Bed Bench, 6 (Suppl 1), S112.
33. Kılıçarslan G, & Dönmez N, 2016: The Effects of Quercetin on Antioxidant System and Some Blood Parameters in Experimental Diabetic Rats. Bull Env Pharmacol Life Sci, 5, 28-32.
34. Kilincarslan G, & Donmez N, 2019: Effect of Quercetin Administration and Exercise on Plasma Cytokine Levels in Rats with STZ Induced Diabetes. Bull. Env. Pharmacol. Life Sci, 8, 119-127.
35. Kumar D, Priya V, Jaiprabhu J, & Ramalingam K, 2014: Serum copper and zinc levels significance in type 2 diabetic patients. J Med Sci Tech, 3 (2), 79-81.
36. Lenaerts AJ, Johnson CM, Marrieta KS, Gruppo V, & Orme IM, 2005: Significant increases in the levels of liver enzymes in mice treated with anti-tuberculosis drugs. Int J Antimicrob Agents, 26 (2), 152-158.
37. Lippi G, Schena F, Montagnana M, Salvagno GL, Banfi G, & Guidi GC, 2011: Significant variation of traditional markers of liver injury after a half-marathon run. Eur J Intern Med, 22 (5), e36-e38.
38. Liu G, & Wang XH, 2014: Research advances in the effects of excise and diet on LPL and its mechanism. Sheng li ke xue jin zhan [Progress in physiology], 45 (2), 87-92.
39. Makinde EA, Radenahmad N, Adekoya AE, & Olatunji OJ, 2020: Tiliacora triandra extract possesses antidiabetic effects in high fat diet/streptozotocin‐induced diabetes in rats. J Food Biochem, 44 (6), e13239.
40. Malawadi B, & Adiga U, 2016: Plasma proteins in type 2 diabetes mellitus. J Biotechnol Biochem, 2 (5), 1-3.
41. Mohamed J, Nafizah AN, Zariyantey A, & Budin S, 2016: Mechanisms of diabetes-induced liver damage: the role of oxidative stress and inflammation. Sultan Qaboos Univ Med J, 16 (2), e132.
42. Mohammad P, Esfandiar KZ, Abbas S, & Ahoora R, 2019: Effects of moderate-intensity continuous training and high-intensity interval training on serum levels of resistin, chemerin and liver enzymes in streptozotocin-nicotinamide induced type-2 diabetic rats. J Diabetes Metab Disord, 18, 379-387.
43. Mooren F, Krüger K, Völker K, Golf S, Wadepuhl M, & Kraus A, 2011: Oral magnesium supplementation reduces insulin resistance in non‐diabetic subjects–a double‐blind, placebo‐controlled, randomized trial. Diabetes Obes Metab, 13 (3), 281-284.
44. O’hagan C, De Vito G, & Boreham CA, 2013: Exercise prescription in the treatment of type 2 diabetes mellitus. Sports med, 43(1), 39-49.
45. Perego C, Da Dalt L, Pirillo A, Galli A, Catapano AL, & Norata GD, 2019: Cholesterol metabolism, pancreatic β-cell function and diabetes. Biochimi Biophys Acta mol basis dis, 1865(9), 2149-2156.
46. Pettersson J, Hindorf U, Persson P, Bengtsson T, Malmqvist U, Werkström V, & Ekelund M, 2008: Muscular exercise can cause highly pathological liver function tests in healthy men. Br J Clin Pharmacol, 65(2), 253-259.
47. Pourvaghar MJ, & Shahsavar AR, 2010: The changes of serum magnesium on nano scale after an exhaustion exercise in male students. Dig J Nanomater Biostructures, 5 (1).
48. Qureshi A, Jamil M, Butt MZI, Khan S, Khaskheli NA, Darya MH, Selamoglu Z, & Ozdemir B, 2022: Impact of High-Intensity Exercise on Antioxidant System and Liver Enzymes: High-Intensity Exercise, Antioxidant System & Liver Enzymes. PBMJ, 31-36.
49. Ragbetli C, Dede S, Tanritanir P, Yoruk IH, & Ragbetli MC, 2014: Determination of micronutrients and oxidative stress status in the blood of STZ-induced experimental diabetic rat models. Cell biochem biophys, 70, 933-938.
50. RamzanPour MR, Mousavian S, & Hejazi SM, 2014: The effect of 12 weeks of selective aerobic exercise on insulin resistance and hepatic enzymes in middle-aged type II diabetic women. Int J Sport Stud, 4 (5), 508-513.
51. Rana S, Morya RK, Malik A, Bhadada SK, Sachdeva N, & Sharma G, 2016: A relationship between vitamin D, parathyroid hormone, calcium levels and lactose intolerance in type 2 diabetic patients and healthy subjects. Clin Chim Acta, 462, 174-177.
52. Riaz S, Tariq M, & Aslam S, 2018: Association of serum protein levels in the diabetic patients with risk of cardiovascular disease and nephropathy in Pakistani population. J Res Diabetes Metab, 4 (1), 011-015.
53. Sjögren M, 2007: ADVANCES IN HEPATOLOGY. Gastroenterol Hepatol, 3 (12), 913.
54. Tangvarasittichai S, 2015: Oxidative stress, insulin resistance, dyslipidemia and type 2 diabetes mellitus. World J Diabetes, 6 (3), 456.
55. Ugurlu I, Baltaci SB, Unal O, Mogulkoc R, Ucaryilmaz H, & Baltaci AK, 2022: Chronic Running Exercise Regulates Cytotoxic Cell Functions and Zinc Transporter SLC39A10/ZIP10 Levels in Diabetic Rats. Biol trace Element Res, 200 (2), 699-705.
56. Yang W, Zhang Y, Yang J, Tan L, & Yang K, 2011: Potential antiosteoporosis effect of biodegradable magnesium implanted in STZ‐induced diabetic rats. J Biomed Mater Res Part A, 99 (3), 386-394.
57. Yao J, Meng M, Yang S, Li F, Anderson RM, Liu C, Liu L, Yuan X, Fang Z, & Lou Q, 2018: Effect of aerobic and resistance exercise on liver enzyme and blood lipids in Chinese patients with nonalcoholic fatty liver disease: a randomized controlled trial. Int J Clin Exp Med, 11(5), 4867-4874.
58. Yaribeygi H, Butler AE, & Sahebkar A, 2019: Aerobic exercise can modulate the underlying mechanisms involved in the development of diabetic complications. J Cell Physiol, 234(8), 12508-12515.
59. Yazdi HB, Hojati V, Shiravi A, Hosseinian S, & Vaezi G, 2019: Liver dysfunction and oxidative stress in streptozotocin-induced diabetic rats: protective role of Artemisia turanica. J Pharmacopuncture, 22(2), 109.