Analysis of Proteins of Corynebacterium pseudotuberculosis Exposed to Starvation Stress by SDS-PAGE

Abstract

Corynebacterium pseudotuberculosis is a Gram-positive facultative bacterium that is an intracellular pathogen causing caseous lymphadenitis (CLA) in small ruminants. In addition, C. pseudotuberculosis is a bacterial species that causes disease in humans and animals. When exposed to starvation stress, this bacterium adapts by changing its cellular structure and metabolism. These changes may also result in alterations in the protein expression of the bacterium. In this study, we aimed to investigate the differences in protein profiles of C. pseudotuberculosis when exposed to starvation stress and when grown on general media. A previously isolated field strain and a reference strain of C. pseudotuberculosis were grown in Tryptic Soy Broth and RPMI 1640 (Roswell Park Memorial Institute), and all proteins were analyzed by SDS PAGE. As a result, it was determined that there were profile differences in protein structures between C. pseudotuberculosis strains exposed and not exposed to starvation stress.

Açlık Stresine Maruz Kalan Corynebacterium pseudotuberculosis’in SDS-PAGE İle Proteinlerinin Analizi

Abstract

Corynebacterium pseudotuberculosis, hücre içi bir patojen olup küçük ruminantlarda kazeöz lenfadenit (KLA) hastalığına sebep olan, Gram-pozitif fakültatif bir bakteridir. Ayrıca C. pseudotuberculosis, insanlarda ve hayvanlarda hastalık oluşturan bir bakteri türüdür. Bu bakteri, açlık stresine maruz kaldığında, hücresel yapısını ve metabolizmasını değiştirerek adaptasyon gösterir. Bu değişiklikler, bakterinin protein ekspresyonunda da değişikliklere neden olabilir. Bu çalışmada, C. pseudotuberculosis’in açlık stresine maruz bırakıldığında ve genel besiyerlerinde üretildiğinde protein profillerinde meydana gelen farklılıkların araştırılması amaçlanmıştır. Daha önce izole edilmiş olan bir saha suşu ve referans olarak kullanılan C. pseudotuberculosis suşu Tryptic Soy Broth ve RPMI 1640 (Roswell Park Memorial Institute) besiyerlerinde üretildikten sonra tüm proteinleri SDS PAGE ile karşılaştırılarak incelendi. Sonuç olarak, açlık strese maruz bırakılan ve bırakılmayan C. pseudotuberculosis suşları arasında protein yapıları arasında profil farklılıkları olduğu tespit edilmiştir.

Keywords

• Corynebacterium pseudotuberculosis
• RPMI
• SDS-PAGE
• stres

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