DEKSKETOPROFEN TROMETAMOLÜN SIÇAN BÖBREĞİ ÜZERİNDEKİ TOKSİK ETKİSİNE KARŞI C VİTAMİNİN KORUYUCU VE ÖNLEYİCİ ETKİSİNİN ARAŞTIRILMASI

Abstract

Günümüzde birçok ağrı kesici kullanılmaktadır. Bunların önemli bir kısmı nonsteroid antiinflamatuar ilaçlardır (NSAİİ). Deksketoprofen trometamol (DEX), NSAİİ grubundan bir ağrı kesicidir. Sık ve yüksek doz kullanımı böbrekler üzerinde toksik etkilere sahiptir. C vitamini (Vit C) güçlü bir antioksidandır. Bu çalışmada, Vit C’ nin DEX' in böbrek toksisitesine karşı koruyucu ve önleyici etkileri araştırılmıştır. Mevcut çalışmada, böbrek toksisitesine, apoptoza ve lipid peroksidasyonuna neden olan biyobelirteçler değerlendirilmiştir. DEX grubunda, kontrol grubuyla karşılaştırıldığında; Üre, kreatinin, malondialdehit (MDA), interlökin-17A (IL-17A), nükleer faktör kappa-B (NF-κB), tümör nekroz faktörü-α (TNF-α), kaspaz-3, Bcl-2 ile ilişkili X proteini (Bax) ve böbrek hasarı molekülü-1 (KIM-1) değerleri daha yüksek, glutatyon (GSH), süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GPx), B hücreli lenfoma-2 (Bcl-2) ve aquaporin-2 (AQP-2) değerleri ise daha düşük bulundu. DEX+C Vitamini grubu DEX grubuyla karşılaştırıldığında üre, kreatinin, MDA, IL-17A, NF-κB, TNF-α, kaspaz-3, Bax ve KIM-1 değerleri daha düşük bulunurken, GSH, SOD, CAT, GPx, Bax ve AQP-2 değerleri daha yüksek bulundu. Kontrol grubuyla karşılaştırıldığında, DEX grubunda renal toksisite, apoptozis ve lipid peroksidasyon biyobelirteçlerinde artışlar gözlenirken, DEX+C Vitamini grubunda tam tersi sonuçlar gözlendi. Bu sonuçlar C Vitamininin DEX nefrotoksisitesine karşı koruyucu olduğunu gösterdi.

Keywords

• Apoptozis
• Deksketoprofen trometamol
• Nefrotoksisite
• Oksidatif stres
• C vitamini

Investigation of The Protective and Preventive Effect of Vitamin C Against the Toxic Effect of Dexketoprofen Trometamol on Rat Kidney

Abstract

There are many painkillers used today. A significant portion of these are nonsteroidal anti-inflammatory drugs (NSAIDs). Dexketoprofen trometamol (DEX) is an analgesic drug from the NSAID group. Frequent and high-dose use has toxic effects on the kidneys. Vitamin C (Vit C) is a powerful antioxidant. The current study investigated the protective and preventive effects of Vit C against the kidney toxicity of DEX. In this study, biomarkers associated with kidney toxicity, apoptosis and lipid peroxidation were evaluated. In the DEX group, compared to the control group; Urea, creatinine, malondialdehyde (MDA), interleukin-17A (IL-17A), nuclear factor kappa-B (NF-κB), tumor necrosis factor-α (TNF-α), caspase-3, Bcl-2-associated X protein (Bax) and kidney injury molecule-1 (KIM-1) values were higher, while glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), B cell lymphoma-2 (Bcl-2) and aquaporin-2 (AQP-2) values were lower. When the DEX+Vit C group was compared with the DEX group, urea, creatinine, MDA, IL-17A, NF-κB, TNF-α, caspase-3, Bax and KIM-1 values were found lower, while GSH, SOD, CAT, GPx, Bax and AQP-2 values were found higher. Compared to the control group, the DEX group showed increases in biomarkers of renal toxicity, apoptosis, and lipid peroxidation, while the opposite results were observed in the DEX+Vit C group. These results demonstrated that Vit C protects against DEX nephrotoxicity.

Keywords

• Apoptosis
• Dexketoprofen trometamol
• Nephrotoxicity
• Oxidative stress
• Vitamin C.

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