Cephalaria procera, Scorzonera tomentosa ve Hedwigia ciliata ekstraktlarının in vitro antimikrobiyal aktivitelerinin değerlendirilmesi

Abstract

Bu çalışmada, artan antimikrobiyal direnç karşısında veteriner hekimlikte yeni tedavi seçenekleri geliştirme amacıyla, Türkiye'ye özgü endemik bitkiler olan Cephalaria procera, Scorzonera tomentosa ve Hedwigia ciliata ekstraktlarının antimikrobiyal aktiviteleri in vitro koşullarda değerlendirildi. Bitki ekstraktları veteriner hekimlik açısından klinik öneme sahip çeşitli bakteri ve mantar suşları üzerinde test edildi. Ekstraktların antibakteriyel etkilerini belirlemek için agar dilüsyon yöntemi, antifungal etkilerini belirlemek için sıvı makrodilüsyon yöntemi kullanıldı. C. procera ekstraktı Staphylococcus epidermidis, Bacillus subtilis ve E. coli üzerinde 2,2 mg/mL konsantrasyonda antibakteriyel etki gösterirken; S. tomentosa bakterilere karşı kullanılan en yüksek konsantrasyonlarda etkisiz bulundu. C. procera ve S. tomentosa Microsporum canis üzerinde sırasıyla 27,5 mg/mL ve 16,25 mg/mL düzeylerinde sınırlı antifungal aktivite gösterdi. H. ciliata ise test edilen tüm mikroorganizmalara karşı kullanılan en yüksek konsantrasyonlarda dahi etkisiz bulundu. Bu bulgular, Türkiye'ye özgü bu endemik bitkilerin antimikrobiyal potansiyellerinin sınırlı olduğunu ancak gelecekte yapılacak daha kapsamlı çalışmalarla, farklı ekstraklarla sinerjik etkileri geliştirilerek alternatif tedavi seçenekleri arasında değerlendirilebileceğini göstermektedir.

Thanks

Cephalaria procera ve Scorzonera tomentosa bitki örneklerin toplanması ve ayıklanmasında yaptıkları katkılardan dolayı Sayın Haydar METİNER ve Sayın Zülfü SADAĞ’ a teşekkür ederiz.

Evaluation of the In Vitro Antimicrobial Activities of Cephalaria procera, Scorzonera tomentosa, and Hedwigia ciliata Extracts

Abstract

The present study evaluated the antimicrobial potential of extracts obtained from Cephalaria procera, Scorzonera tomentosa, and Hedwigia ciliata, endemic plants specific to Türkiye, in vitro to develop new treatment options in veterinary medicine in the face of increasing antimicrobial resistance. The plant extracts were tested on various bacterial and fungal strains of clinical importance in veterinary medicine. To determine antibacterial efficacy, the extracts were tested using an agar dilution method; antifungal activity was assessed using a  broth macrodilution technique. C. procera extract exhibited inhibitory effects on Staphylococcus epidermidis, Bacillus subtilis, and E. coli at a concentration of 2.2 mg/mL; while S. tomentosa was found to be ineffective against bacteria at the highest concentrations used. C. procera and S. tomentosa showed limited antifungal activity against Microsporum canis at levels of 27.5 mg/mL and 16.25 mg/mL, respectively. H. ciliata was found to be ineffective against all tested microorganisms, even at the highest concentrations used. These findings indicate that the antimicrobial potential of these endemic plants unique to Türkiye is limited, but they could be considered as alternative treatment options through more comprehensive studies in the future by developing synergistic effects with different extracts.

Keywords

• Antibacterial
• antifungal
• Cephalaria procera
• Hedwigia ciliata
• in vitro
• Scorzonera tomentosa

Thanks

We would like to thank Mr. Haydar METİNER and Mr. Zülfü SADAĞ for their contributions to the collection and sorting of Cephalaria procera and Scorzonera tomentosa plant specimens.

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