Investigation of Immunological Infertility in Repeat Breeder Cows

Abstract

In terms of the future of livestock businesses, obtaining calf from cows is crucial. There are many factors that prevent fertility. These factors include hereditary and functional problems (an- oestrus, sub-oestrus, cysts), infectious diseases, and miscarriage. It is noteworthy that some cows do not become pregnant despite the numerous inseminations even in the absence of an appearent reason. In this study it was aimed to investigate the incidence of immunological infertility by determining immunglobulin levels in repeat breeder cows. In the study 50 cows which calved at least once and were not pregnant despite at least 3 subsequent inseminations although they did not have any disorders preventing fertility as assessed by rectal and ultrasonographic examinations, were used. Antisperm antibodies (ASA) were detected by ELISA test in blood serum samples obtained after insemination. As a result, ASA were detected in 14.33% of the cows that were at least 3 times inseminated but not conceived at any time in normal field conditions.

Keywords

• Infertility,Immunological infertility,Secretory immuneglobulin A,Artificial insemination

Döl Tutmayan İneklerde İmmunolojik İnfertilitenin Araştırılması

Abstract

Hayvancılık işletmelerinin geleceği açısından ineklerden yavru elde edilmesi çok önemli yer teşkil eder. İneklerde infertilite nedenleri çok çeşitlidir. Bunlar arasında herediter, işlevsel (anöstrus, suböstrus, kistler), enfeksiyöz hastalıklar, bakım ve besleme bozuklukları sayılabilir. Günümüzde yukarıda sayılan bu nedenlerin bulunmadığı durumlarda bile yapılan çok sayıda tohumlamaya rağmen bazı ineklerde gebe kalamama olgularının görülmesi dikkat çekicidir. Bu çalışmada infertil ineklerde immunglobulin seviyelerine bakılarak immünolojik infertilitenin tespiti ve immünolojik infertilitenin görülme sıklığının araştırılması amaçlanmıştır. Çalışmada, özel işletmelerde bulunan ve en az 3 kere tohumlandığı halde gebe kalmayan, rektal ve ultrason ile yapılan muayenede fertiliteye engel herhangi bir bozukluğu bulunmayan, daha önce en az bir kere sorunsuz doğum yapmış inekler kullanılmıştır. Tohumlama sonrası elde edilen kan serumu örneklerinde ELISA testi ile antisperm antikorları tespit edilmiştir. Sonuç olarak, normal saha koşullarında test edilen herhangi bir rahatsızlığı bulunmayan, en az 3 kere tohumlanmış ama gebe kalmamış ineklerin kan serumlarında ELISA testi ile % 14,33 oranında antisperm antikorları (ASA) tespit edilmiştir. 

Keywords

• İnfertilite,İmmunolojik infertilite,Salgısal immunglobülin A,Suni tohumlama

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