Deve (Camelus dromedarius) İnce Barsak Mukozasında Plazma Hücresi ve Eozinofil Granulosit Dağılımının Belirlenmesi

Abstract

Yapılan çalışmada develerde ince barsak mukozasında plazma hücresi ve eozinofil granulositlerin lokalizasyon ve dağılım oranlarının ortaya konulması amaçlanmıştır. Çalışmada kesimhaneden elde edilen erişkin ve sağlıklı altı adet tek hörgüçlü deveden alınan ince barsak (duodenum, jejenum, ileum) örnekleri kullanıldı. Alınan doku örnekleri %10 NBF (Neutral buffer formalin) ve IFAA ( İzotonik formaldehit asetik asit) (Ph 2.9) solüsyonlarında tespit edildi. Rutin histolojik işlemleri takiben parafinde bloklanan dokulardan 6m kalınlığında seri kesitler alındı. Elde edilen kesitlere plazma hücrelerini belirlemek amacıyla MGP (Methyl green pyronin), eozinofil granulositleri belirlemek amacıyla Kongo Red ve genel görünüm için Crossmon’ın üçlü boyama yöntemleri uygulandı. Yapılan çalışma ile duodenumdaki plazma hücrelerinin; villusların tepe kısmında yoğunlaştığı (4-en yoğun), az sayıda olan eozinofil granulositlerin ise; kan damarları etrafında toplandığı (2-az yoğun) belirlendi. Jejenumda ise; plazma hücrelerinin sayılarının duodenuma göre azaldığı (3-yoğun) ve villus-kript aralığında yoğunlaştığı belirlenirken (3-yoğun), eozinofil granulosit sayılarının villuslarda arttığı  (4-en yoğun) gözlendi. İleumda; villuslarda plazma hücreleri sayısının arttığı  (4-en yoğun) gözlendi. Ayrıca agregat lenf folliküllerinde de plazma hücreleri dikkati çekti. Bununla birlikte eozinofil granulositlerin ileum villuslarında yer aldığı (3-yoğun) belirlendi. Bu hücrelere az oranda lenf folliküllerinde de rastlandı. Sonuç olarak yapılan çalışma ile ince barsaklarda hem plazma hücrelerinin hem de eozinofil granulositlerin genel olarak villusların tepe kısmında yoğunlaştığı belirlenmiştir. Bununla birlikte lenf folliküllerinde de plazma hücreleri ve eozinofil granulositlerin yerleştiği saptanmıştır. Paraziter enfeksiyonlarda sayıları artan plazma hücreleri ve eozinofil granulositlerin bağırsak sağlığının korunmasında önemli rol oynadığı kanaatine varılmıştır.

Keywords

• Deve,Eozinofil granulosit,İnce barsak,Plazma hücresi

Determination of the Plasma Cells and Eosinophil Granulocytes Distribution in the Small Intestine Mucosa of the Camel (Camelus dromedarius)

Abstract

The present study aimed the investigation of the plasma cells and eosinophil granulocyte localization and distrubution in the small intestine mucosa of the camel (Camelus dromedarius).  Small intestine samples (duodenum, jejenum, ileum) of six adult and healty one-humped camel were obtained from the slaughterhouse . Tissue samples were fixed by immersion in 10% neutral formalin saline and IFAA (isotonic formalin acetic acid) Ph:2,9. After routine histological procedure tissues embedded in paraffin. Sections (6m-thick) were stained methyl-green pyronin (MGP) for plasma cells, Congo Red for eosinophil granulocytes and Masson’s trichrome stain modified by Crossmon for general view. In duodonum the plasma cells were concentrated at the tip of villi (4-most intense) while few eosinophil granulocytes (2-less intense) that concentrated around the blood vessels were observed. The number of plasma cells were decreased in villus of jejenum (3-intense), at the same time plasma cells number in villus-crypt space were increased (3-intense). Eosinophil granulocytes were increased in villus of jejenum (4-most intense). In ileum, increased plasma cell numbers were observed in villus (4-most intense). Plasma cells were determined in aggregated lymph follicles. Eosinophil granulocytes were seen in villus of ileum (3-intense) while these cells were rarely observed in lymph follicles. As a result, it was determined that both plasma cells and eosinophil granulocytes were concentrated in the villi in the small intestines. In addition, plasma cells and eosinophil granulocytes were found to be located in the lymph follicles. It has been concluded that plasma cells and eosinophil granulocytes with increasing numbers of parasitic infections play an important role in preserving the intestinal health.

Keywords

• Camel,Eosinophil granulocytes,Small intestine,Plasma cells

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