Bir Ankara Kedisi’nde Malign Fibröz Histiositomun (Dev Hücreli Tip) İnce İğne Aspirasyon Sitolojisi

Abstract

Bu olguda, 9 yaşlı, Ankara ırkı, dişi bir kedinin sırtında radyolojik, sitopatolojik ve histopatolojik olarak dev hücreli malign fibröz histiyositoma olgusu tanımlandı. Latero-lateral ve ventro-dorsal olarak  tümöral kitlenin invazyonu radyolojik olarak belirlendi. Radyolojik incelemede kitle invaziv görüntüde olup kemiğe bağlantısı yoktu.  Genel anestezi altında tümöral bölgeden İnce İğne Aspirasyon Sitolojisi (İİAS) tekniğine göre sitopatolojik örnekler alınarak May-Grunwald Giemsa yöntemine göre boyandı. Cerrahi operasyonla uzaklaştırılan biyopsi örneği ise rutin doku takibine alınarak hematoksilen eosin (HE) ile boyandı. Biyopsi materyali 8x6x6 cm ve 95 gr ağırlığında, deriyle kaplı, multilobuler yapıda ve sert kıvamlıydı. Kesit yüzü gri beyaz ve nekrotik manzaradaydı. Sitopatolojik incelemelerde anaplazik özelliklerde, pleomorfik şekilli histiyosit benzeri yada fibrosit benzeri çok sayıda hücreyle beraber, geniş ve vakuoler sitoplazmalı, çok çekirdekli dev hücreleri dikkati çekti. Histolojik incelemelerde ise, çeşitli yönlere girdaplar şeklinde dizilim yapmış atipik özellikler gösteren histiyosit benzeri veya bağ dokusu hücrelerinin aralarında, çok sayıda, geniş sitoplazmalı, 8-12 çekirdekli dev hücreleri fark edildi.

Keywords

• Kedi,Sitoloji,Radyoloji,Histopatoloji,Malign fibröz histiositom

Fine-Needle Aspiration Cytology of Malignant Fibrous Histiocytoma (Giant Cell Type) in an Angora Cat

Abstract

This case study presents the radiological, cytopathological and histopathological description of the giant cell type of a malignant fibrous histiocytoma on the back of a 9-year-old female Angora cat. Latero-lateral and ventro-dorsal thoracic radiographs was taken to evaluate invasion. Radiologically the mass was invasive but had not invaded the bone. Under general anaesthesia, cytopathological samples were taken from the tumour, using the Fine-Needle Aspiration Cytology (FNAC) technique, and were later stained with May-Grünwald Giemsa solution. The surgically extracted biopsy sample was subjected to routine tissue processing and stained with Haematoxylin-Eosin (HE). The biopsy material was 8x6x6 cm and 95 g, was covered by skin, multilobulary appearance and hard consistency. Cross-sections was greyish white and necrotic appearance. Cytopathological examination revealed the presence of numerous histiocyte- or fibrocyte-like cells of anaplastic character and pleomorphic shape, which were associated with giant cells with multiple nuclei and a broad and vacuolar cytoplasm. Histological examination demonstrated the presence of histiocyte-like atypical cells arranged in the form of swirls and extending in various directions, or numerous giant cells with 8 to 12 nuclei and a broad cytoplasm situated in between connective tissue cells.

Keywords

• Cat,Cytology,Radiology,Histopathology,Malignant fibrous histiocytoma

References

1. Aydın Y, Vural SA, Öznur N, 2003: Bir kedide dev hücreli malign fibröz histiyositom. Ankara Üniv Vet Fak Derg, 50, 247-249.
2. Bullough PG, 1997: Orthopaedic Pathology, 3rd edition, Time Mirror International Publishers Ltd., London.
3. Datarkar A, Hazare VK, 2009: Malignant fibrous histiocytoma: a case report. J Maxillofac Oral Surg, 8, 196-198.
4. Enneking WF, 1990: Clinical musculoskeletal pathology, 3rd revised edition, University of Florida Press/J. Hillis Miller Health Science Center, Gainesville, Florida, 1990.
5. Ford GH, Empson RN, Plopper CG, Brown PH, 1975: Giant cell tumor of soft parts: A report of an equine and a feline case. Vet Pathol, 12, 428-433.
6. Gleiser CA, Raulston GL, Jardine JH, Gray KN, 1979: Malignant fibrous histiocytoma in dogs and cats. Vet Pathol, 16, 199-208.
7. Goldschmidt MH, Shofer FS, 1992: Skin Tumors of the Dog and Cat. Pergamon Press, Oxford, 175-178.
8. Guccion, JG, Enzinger, FM, 1972: Malignant giant cell tumor of soft parts: an analysis of 32 cases. Cancer, 29, 1518-1528.

9. Hamir AN, 1989: Equine giant cell tumor of soft tissues. Cornell Vet, 2, 173-177.
10. Kiran MM, Karaman M, Hatipoglu F, Koc Y, 2005: Malignant fibrous histiocytoma in a dog: a case report. Vet Med Czech, 50, 553-557.
11. Meuten DJ, 2002: Tumors in Domestic Animals. 233-237. In: DJ Meuten (ed), 4th ed. Iowa.
12. Morris JS, Mcinnes EF, Bostock DE, Hoather TM, Dobson JM, 2002: Immunohistochemical and histopathologic features of 14 malignant fibrous histiocytomas from Flat-Coated Retrievers. Vet Path, 39, 473-479.
13. Pobirci DD, Bogdan FL, Pobirci OANA, Petcu CA, Rosca ELENA, 2011: Study of malignant fibrous histiocytoma: clinical, statistic and histopatological interrelation. Rom J Morphol Embryol, 52, 385-388.
14. Turk NS, Kelten C, Ozdemir NO, Duzcan E, 2010: Primary malignant fibrous histiocytoma of the kidney: Report of a case. Turk J Urol, 26, 165-167.
15. Wellman ML, 1990: The cytologic diagnosis of neoplasia. Vet Clin North Am Small Anim Prac, 20, 919-937.