Nil Tilapia (Oreochromis niloticus Linnaeus, 1758) Rasyonuna Vitamin E (Alfa-Tokoferol Asetat) Katkısının Bazı Spermatolojik Parametreler ve Malondialdehid Düzeyine Etkisi

Abstract

Bu çalışmada, Nil Tilapia balıklarının rasyonuna vitamin E (Alfa-tokoferol Asetat) katkısının spermatolojik parametreler (sperm motilitesi, yoğunluğu ve pH’sı) ve malondialdehit (MDA) düzeyleri üzerine olan etkisi araştırıldı. Çalışmada her bir deneme grubunda 12 balık olmak üzere toplam 48 tilapia balığı kullanıldı. Balıkların beslenmesi için %32 oranında ham protein içeren bir kontrol rasyonu hazırlandı. Bu rasyona 80 mg/kg, 160 mg/kg ve 240 mg/kg miktarlarında vitamin E ilave edilerek sırasıyla D1, D2 ve D3 nolu deneme rasyonları oluşturuldu. Tilapia balıklarının canlı ağırlıklarının %4’ü oranında hazırlanan rasyon, 3 ay süreyle günde üç öğün halinde 25±1 °C su sıcaklığında verildi. Çalışmanın sonunda, D1 ve D3 gruplarında kontrol grubuna göre MDA düzeylerinin sayısal olarak arttığı ancak bu artışın istatistiksel olarak önemsiz olduğu belirlenmiştir (p>0,05). D2 grubunda sperm motilitesinin ve sperma pH’nın sayısal olarak arttığı, ancak bu artışın istatistiksel olarak önemsiz olduğu (p>0,05) belirlenmiştir.

Keywords

• Nil tilapia,Vitamin E,Malondialdehid,Spermatolojik parametreler,Alfa-tokoferol asetat

The Effect of Vitamin E (Alpha-Tocopherol Acetate) Supplements to Nile Tilapia (Oreochromis niloticus Linnaeus, 1758) Ration on Some Spermatological Parameters and Malondialdeyhde Level

Abstract

In this study, the effect of vitamin E (Alpha-tocopherol Acetate) contribution to the ration of Nil Tilapia fish on spermatological parameters (sperm motility, density and pH) and malondialdehyde (MDA) levels were investigated. In the study, a total of 48 tilapia fish, 12 fish in each experimental group, were used. A control diet containing 32% crude protein was prepared for feeding the fish. To this diet, 80 mg/kg, 160 mg/kg and 240 mg/kg amounts of vitamin E were added to form D1, D2 and D3, respectively. The ration prepared at the rate of 4% of the live weights of Tilapia fish was given at 25±1 °C water temperature in three times a day for 3 months. At the end of the study, it was determined that MDA levels were increased numerically in D1 and D3 groups, but this increase was statistically insignificant (p>0.05). It was determined that sperm motility and sperm pH were increased numerically in the D2 group, but this increase was statistically insignificant (p> 0.05).

Keywords

• Nile Tilapia,Vitamin E,Malondialdehyde,Spermatological parameters,Alpha-Tocopherol Acetate

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