Deneysel Diyabetik Nefropatide Patomorfoloji ve Apoptozis

Nihat Yumuşak

doi:10.31196/huvfd.686659

Deneysel Diyabetik Nefropatide Patomorfoloji ve Apoptozis

Abstract

Bu çalışmada diyabetik nefropatiye bağlı oluşan böbrek hasarında histopatolojik ve apoptotik bulguların değerlendirilmesi amaçlandı. Çalışmada 16 rat rastgele olarak kontrol (n=8) ve üç gün arayla iki kez streptozisinle (60 mg/kg, i.p) uyarılmış diyabet (n=8) olmak üzere iki gruba ayrıldılar. Son uygulamayı takip eden 90. günde ratlar uyutularak böbrekleri patolojik incelemeler amacıyla alındı. Dokulara histopatolojik olarak hematoksilen-eozin ve immunohistokimyasal olarak kaspas-3, kaspas-8, kaspas-9 ve TUNEL işaretlemeleri yapıldı. Histopatolojik olarak, tubullerde dilatasyon, atrofi, dejenerasyon, nekroz, glomeruluslarda ise hipertrofi ve vasküler değişikler görüldü. İntersitisyel alanlarda ise yer yer peritubuler, perivasküler ve periglomeruler fibrozis ve inflamasyonlar gözlendi. İmmunohistokimyasal olarak, kaspas-3’ün birçok tubulde pozitif olduğu ayrıca glomerullerde ve intersitisyumda tek tük pozitif hücre ile karşılaşıldı. Kaspas-8 ve kaspas-9 antikorlarının ise birçok tubulde reaktif oldukları belirlendi. TUNEL boyamalarında ise yaygın pozitiflik tubulerde, yer yer intersitisyumda ve glomeruluslarda belirlenmiştir. Sonuç olarak, diyabete bağlı oluşan nefropatide bütün böbrek dokusunda hasarın şekillendiği belirlenmiştir.

Keywords

Diyabetik nefropati,patomorfoloji,apoptozis

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Details

Primary Language

Turkish

Subjects

Veterinary Surgery

Journal Section

Research Article

Authors

Nihat Yumuşak ^*
0000-0002-9299-2902
Türkiye

Publication Date

June 25, 2020

Submission Date

February 8, 2020

Acceptance Date

March 17, 2020

Published in Issue

Year 2020 Volume: 9 Number: 1

DOI

https://doi.org/10.31196/huvfd.686659

IZ

https://izlik.org/JA33HY93ZR

Cite

RIS / Bibtex

APA

Yumuşak, N. (2020). Deneysel Diyabetik Nefropatide Patomorfoloji ve Apoptozis. Harran University Journal of the Faculty of Veterinary Medicine, 9(1), 29-35. https://doi.org/10.31196/huvfd.686659

AMA

1.Yumuşak N. Deneysel Diyabetik Nefropatide Patomorfoloji ve Apoptozis. Harran Univ Vet Fak Derg. 2020;9(1):29-35. doi:10.31196/huvfd.686659

Chicago

Yumuşak, Nihat. 2020. “Deneysel Diyabetik Nefropatide Patomorfoloji Ve Apoptozis”. Harran University Journal of the Faculty of Veterinary Medicine 9 (1): 29-35. https://doi.org/10.31196/huvfd.686659.

EndNote

Yumuşak N (June 1, 2020) Deneysel Diyabetik Nefropatide Patomorfoloji ve Apoptozis. Harran University Journal of the Faculty of Veterinary Medicine 9 1 29–35.

IEEE

[1]N. Yumuşak, “Deneysel Diyabetik Nefropatide Patomorfoloji ve Apoptozis”, Harran Univ Vet Fak Derg, vol. 9, no. 1, pp. 29–35, June 2020, doi: 10.31196/huvfd.686659.

ISNAD

Yumuşak, Nihat. “Deneysel Diyabetik Nefropatide Patomorfoloji Ve Apoptozis”. Harran University Journal of the Faculty of Veterinary Medicine 9/1 (June 1, 2020): 29-35. https://doi.org/10.31196/huvfd.686659.

JAMA

1.Yumuşak N. Deneysel Diyabetik Nefropatide Patomorfoloji ve Apoptozis. Harran Univ Vet Fak Derg. 2020;9:29–35.

MLA

Yumuşak, Nihat. “Deneysel Diyabetik Nefropatide Patomorfoloji Ve Apoptozis”. Harran University Journal of the Faculty of Veterinary Medicine, vol. 9, no. 1, June 2020, pp. 29-35, doi:10.31196/huvfd.686659.

Vancouver

1.Nihat Yumuşak. Deneysel Diyabetik Nefropatide Patomorfoloji ve Apoptozis. Harran Univ Vet Fak Derg. 2020 Jun. 1;9(1):29-35. doi:10.31196/huvfd.686659