Hipotiroidili Ratlarda Koenzim Q10'un Plazma Parametreleri Üzerine Etkisi

Abstract

Deneysel olarak hipotiroidizm oluşturulmuş ratlarda Koenzim Q10 (KoQ10)’un kan glikoz, total protein, trigliserit ve total kolesterol düzeyleri üzerindeki etkisinin belirlenmesi amaçlandı. Araştırmada materyal olarak sağlıklı toplam 32 adet yetişkin erkek Wistar Albino ırkı rattan yararlanıldı. Hayvanların10 günlük adaptasyon süresini takiben esas araştırma dönemi toplam 3 hafta sürdürüldü. Çalışma süresince her kafeste 4 rat olacak şekilde barındırılan ratlar kontrol (K), KoQ10 (C), Hipotiroid (H) ve KoQ10+ Hipotiroid (CH) olmak üzere 4 deneme grubuna ayrıldı. C grubu hayvanlara her gün intraperitoneal olarak KoQ10 uygulandı. Deneysel hipotiroidizm oluşturmak amacıyla, H grubundaki ratların sularına günlük propiltiyourasil (PTU) ilave edildi. HC grubundaki hayvanlara ise hem KoQ10, hem de PTU uygulaması yapıldı. Deneme sonunda gruplardaki deneklerden genel anestezi altında kardiak punksiyon ile yeterli oranda kan alındı ve plazmaları elde edildi. Elde edilen plazma örneklerinden glikoz, total protein, trigliserit ve total kolesterol düzeyleri belirlendi. Araştırmada, hipotiroidizm oluşturulmasının, kontrol grubu ile kıyaslandığında plazma glikoz ve trigliserit değerlerini düşürdüğü (p<0,05), total protein ve total kolesterol düzeylerini arttırdığı (p<0,05) gözlendi. Çalışmada, KoQ10 tedavisinin (HC grubu), hipotiroidizm oluşturulan ratlar ile kıyaslandığında (H grubu) plazma glikoz, total protein ve trigliserit değerleri üzerinde istatistiksel öneme sahip bir etkisi belirlenemezken, total kolesterol düzeyini artırdığı görüldü(p<0,05). Araştırmada KoQ10 (C grubu) uygulamasının, K grubuna göre plazma trigliserit değerlerini azalttığı (p<0,05) görüldü. Sonuç olarak hipotiroidide, CoQ10 uygulaması toplam kolesterol düzeyini arttırdığından, metabolik hastalıklarda CoQ10 takviyesinin kan lipit profili üzerindeki etkisi göz önünde bulundurulmalıdır.

Keywords

• KoQ10
• hipotiroidizm
• plazma parametreleri
• tiroid hormonları
• rat

Effect of Coenzyme Q10 on Plasma Parameters in Hypothyroıd Rats

Abstract

This research was conducted to determine the effect of Coenzyme Q10 (CoQ10) on blood glucose, total protein, triglyceride, and total cholesterol levels in rats for that experimental hypothyroidism was induced. A total of 32 healthy adult male Wistar Albino rats were used as a material in the research. After the adaptation period of animals for ten days, the main research period was conducted for a total of three weeks. The rats housed as four rats in each cage during the research were divided into four experimental groups as control (K), CoQ10 (C), Hypothyroid (H), and CoQ10+Hypothyroid (CH). CoQ10 was administered intraperitoneally every day to the group C animals. In addition, propylthiouracil (PTU) was added daily to the water of rats in group H to induce experimental hypothyroidism. Both CoQ10 and PTU administration was performed on the animals in the group HC. At the end of the trial period, blood was drawn from the hearts of rats in all groups. Glucose, total protein, triglyceride, and total cholesterol levels were determined in plasma samples obtained from blood samples. It was observed in the research that hypothyroidism administration (group H) decreased plasma glucose and triglyceride values (p<0.05) and increased total protein and total cholesterol levels (p<0.05) compared to group K. In the study, it was observed that while CoQ10 administration (group HC) did not have a statistically significant effect on plasma glucose, total protein and triglyceride values in hypothyroidism-induced rats (group H), it increased the total cholesterol level (p<0,05). In the research, it was observed that the CoQ10 administration (group C) decreased plasma triglyceride values compared to that of group K (p<0.05). As a result, since CoQ10 administration increases total cholesterol level in hypothyroidism, the effect of CoQ10 supplementation on blood lipid profile in metabolic diseases should be considered.

Keywords

• CoQ10
• hypothyroidism
• plasma parameters
• thyroid hormones
• rat

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