The promoter effect of laminin-derived IKVAV peptide on three dimensional HUVEC microtissue

Abstract

Tissue engineering research is recently a popular field but the vascularization process of existing methods limits the study area. Human Umbilical Vein Endothelial Cells (HUVEC) are essential cell models for vascularization study in vitro. Although studies about vascular biomaterial are mostly performed in traditional 2 Dimensional (D) cell culture, the system has some disadvantages. However, 3D scaffold-free microtissue can be used to overcome these disadvantages for the identification of the optimum concentration of biomaterials. IKVAV is an active unit of laminin which is an effective protein in the extracellular matrix. IKVAV may increase cell adhesion, proliferation, migration, and cellular differentiation. Since IKVAV directly affects endothelial cells, the definition of the optimum concentration of IKVAV is critically important for HUVEC growth and viability during vascularization. Thus, the study aimed identification of the optimal IKVAV peptide concentration for the production and viability of 3D HUVEC SFM. After peptide synthesis, 3D SFM was fabricated. 0.5 mM and 1 mM concentrations of IKVAV peptide were treated with SFM. The control group was incubated without any peptide concentration. Diameters and viabilities of SFMs were evaluated. 1 mM concentration showed the highest diameter and viability. The increasing concentrations may support HUVEC growth and viability so it may induce vascularization in vivo conditions.

Keywords

• huvec cell line
• IKVAV peptide
• Microtissue

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