Virus diseases of lettuce in ankara province

Abstract

Lettuce is one of the widely grown vegetable in Ankara province of Turkey and virus diseases cause great yield loss.  Surveys were conducted to five different districts of Ankara during two years, 2014-2015 and a total of 544 samples were collected, and all were tested with the DAS-ELISA against Mirafiori lettuce big vein virus (MiLBVV), Lettuce mosaic virus (LMV), Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSVW) antisera. LMV was the predominant and unique infection detected in 2014 and was present in the 65 of the specimen collected but  in 2015, 39 MiLBVV, 25 LMV infected isolates were detected. TSWV and CMV were not present in the research area. RT-PCR tests were performed to determine the coat protein gene regions of those viruses. Amplicons were obtained at 800 bp, 469 bp and 296 bp of LMV, MiLBVV and LBVaV, respectively. Lettuce big vein-associated virus (LBVaV) were present in the 5 out of a total of 6 samples, as mixed infected with MiLBVV. Microscopic observations were performed to determine the presence of Olpidium spp. in the roots which is the vector of the viruses belong to Lettuce Big Vein diseases (LBV), resting spores were observed in 146 of the investigated root samples. These results indicate that LMV and LBV diseases causing viruses are the most common endemic viral pathogens in Ankara's lettuce cultivation areas and the vector Olpidium spp. was present in the research area.

Keywords

• Ankara,lettuce viruses,DAS-ELISA,olpidium spp

References

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