Antioxidant activity, total phenolic compounds and ld50 bioassay (toxicity) activity of the fermented wood “Nikhra” fractions of combretum hartmannianum, terminalia laxiflora and acacia seyal

Abstract

This study characterizes antioxidant activity, total phenolic compounds and LD50 bioassay (Toxicity) of the fermented wood “Nikhra” fractions of Combretum hartmannianum, Acacia seyal and Terminalia laxiflora. Antioxidant potential using DPPH assay, the content of total phenolic compounds in the fractions was determined spectrometrically according to the Folin−Ciocalteu procedure and calculated as gallic acid equivalents (GAE) and toxicity using the brine shrimp Artemia salina standard method. These trees exhibited far stronger antioxidant activity and contained significantly higher levels of phenolics. All fractions proved to be non toxic against A. salina expect ethyl acetate and chloroform fractions of A. seyal and chloroform fractions of C. hartmannianum which possessed slight toxicity.

Keywords

• Antioxidant activity;
• Phenolic compounds;
• Phenolic acids;
• Flavonoids;
• Tannins;
• Free radical;
• Toxicity activity

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