Indocyanine green-mediated photodynamic therapy on glioblastoma cells in vitro

Abstract

Photodynamic therapy (PDT) is an alternative therapy which is administered with non-toxic drugs, called photosensitizers (PSs), along with irradiation at a specific wavelength of light to damage tumor cells. Different wavelengths of light sources and photosensitizers have been investigated in treatment of many cancer types. In this study, we investigated whether photodynamic therapy using indocyanine green (ICG), also a cyanine dye used in medical diagnostics, can be used to inhibit cell proliferation of glioblastoma. Glioblastoma cells were irradiated with a diode laser (λ = 809 nm, 100 J cm⁻²) in continuous wave operation mode. Cell proliferation was measured by XTT assay at 24 h after light irradiation. While only ICG application (10 µg/ml and 50 µg/ml concentrations) had no effects on glioblastoma cell proliferation, ICG in combination with laser irradiation (ICG-PDT) caused a significant decrease of cell proliferation. In conclusion, ICG-PDT may be valuable therapeutic approach as potential treatment for cancer such as glioblastoma. The results could be used as primary data for efficacy of ICG-PDT on malignant tumors.

Keywords

• photodynamic therapy, indocyanine green, glioblastoma, cell proliferation

Original Research Paper

Öz

References

1. A. P. Castano, T.N. Demidovaa, M.R. Hamblin, “Mechanisms in photodynamic therapy: part two-cellular signaling, cell metabolism and modes of cell death,” Photodiagnosis and Photodynamic Therapy, 2, pp. 1-23, 2005.
2. R. Bhuvaneswari, Y.Y. Gan, K.C. Soo and M. Olivo, “Targeting EGFR with photodynamic therapy in combination with Erbitux enhances in vivo bladder tumor response,” Molecular Cancer, 8:94, 2009.
3. D. Bechet, S.R. Mordon, F. Guillemin, M.A. Barberi-Heyob, “Photodynamic therapy of malignant brain tumours: A complementary approach to conventional therapies,” Cancer Treatment Reviews ,40, pp. 229–241, 2014.
4. A. Juzeniene, Q. Peng and J. Moan, “Milestones in the development of photodynamic therapy and fluorescence diagnosis,” Photochem. Photobiol. Sci., 6,pp. 1234–1245, 2007.
5. H.-J. Lim, C.-H. Oh, “Indocyanine green-based photodynamic therapy with 785nm light emitting diode for oral squamous cancer cells,” Photodiagnosis and Photodynamic Therapy, 8, pp. 337-342, 2011.
6. O. Bozkulak, R. F. Yamaci, O. Tabakoglu, M. Gulsoy, “Photo-toxic effects of 809-nm diode laser and indocyanine green on MDA-MB231 breast cancer cells,” Photodiagnosis and Photodynamic Therapy, Vol. 6, Issue 2, pp. 117–121, 2009.
7. C.A. Robertson, D. Hawkins Evans, H. Abrahamse, “Photodynamic therapy (PDT): A short review on cellular mechanisms and cancer research applications for PDT,” Journal of Photochemistry and Photobiology B: Biology, 96, pp. 1–8, 2009.
8. M. Lyons, I. Phang, S. Eljamel, “The effects of PDT in primary malignant brain tumours could be improved by intraoperative radiotherapy,” Photodiagnosis and Photodynamic Therapy, 9, pp. 40-45, 2012.

9. I. Coupienne, S. Bontems, M. Dewaele, N. Rubio, Y. Habraken, S. Fulda, P. Agostinis, J. Piette, “NF-kappaB inhibition improves the sensitivity of human glioblastoma cells to 5-aminolevulinic acid-based photodynamic therapy,” Biochemical Pharmacology, 81, pp. 606–616, 2011.
10. A.R. Montazerabadi, A. Sazgarnia, M.H. Bahreyni-Toosi, A. Ahmadi, A. Aledavood, “The Effects of Combined Treatment with Ionizing Radiation and Indocyanine gren-mediated Photodynamic therapy on Breast Cancer Cells”, Journal of Photochemistry and Photobiology B:Biology, 109, pp. 42-49, 2012.
11. W.W. Tseng, R.E. Saxton, A. Denutti, C.D. Liu, “Infrared Laser Activation of Indocyanine green Inhibits Gorwth in Human Pancreatic Cancer”, Pancreas, 27, pp. 42-45, 2003.
12. W. Baumler, C. Abels, S. Karrer, T. Weib, H. Messmann, M. Landthaler, R.M. Szeimies, “Photo-oxidative Killing of Human ColonicCancer Cells usnig Indocyanine Green and Infrared Light”, British Journal of Cancer, 80, (3/4) pp. 360-363, 1999.
13. Y. Tang, A.J.McGoron, “Combined Effects of Laser-ICG Photothermotherapy and Doxorubicin Chemotherapy on Ovarian Cancer Cells” Journal of Photochemistry and Photobiology B:Biology, 97, pp. 138-144, 2009.
14. K. Skrivanova, J. Skorpikova, J. Svihalek, V. Mornstein, R. Janisch, “Photochemical Properties of a Potential Photosensitiser Indocyanine Green in Vitro”, Journal of Photochemistry and Photobiology B:Biology, 85, pp. 150-154, 2006.