Growth and norharmane production of Chroococcus minutus under various stress conditions

Abstract

Cyanobacterium samples were collected from fresh water of Tokat city in Turkey, and then isolation and cultivation of Chroococcus minutus were achieved successfully. TLC (Thin layer chromatography) and HPLC (High Performance Liquid Chromatography) analyses revealed that the C. minutus consisted of norharmane as a major product. So amount of norharmane was determined during the growth process. Growth and norharmane production of C. minutus were executed under salt stress and pH stress conditions. The most growth and the highest production of norharmane were detected at 16^th day. Therefore inoculation process was performed at 16^th day. Salt stress was evaluated at 0.5, 1.0, 3.0 and 5.0 M concentrations. The most norharmane was synthesized by C. minutus at 5 M concentration. The norharmane production and the growth were higher at pH 9 than that of the pH 5. Most norharmane was produced at pH 7.

Keywords

• Chroococcus minutus,cyanobacteria,HPLC,norharmane

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