Recombinant human G-CSF production as a protein based drug candidate for hematology and oncology

Yasemin Bozkurt; Sema Bilgin; Seçil Erden; İsmail Furkan Turan; İsa Gökçe

doi:10.32571/ijct.600180

EN

Recombinant human G-CSF production as a protein based drug candidate for hematology and oncology

Abstract

The human granulocyte colony stimulating factor (hG-CSF) is a member of the CSF family. The first purpose of this study was production of recombinant human G-CSF (rhG-CSF) which is an important therapeutic protein for angiogenesis based clinical applicaitons. rhG-CSF was produced as inclusion body in the Escherichia coli strain BL21 (DE3) pLysE with pTOLT vector system. The rhG-CSF was purified by Ni-NTA agarose afinity chromatography and characterized with SDS-PAGE analysis. The effects of this therapeutic protein on cell viability was measured by MTT assay. Additionally, angiogenic potential of produced rhG-CSF was investigated via HUVEC cell line by in vitro scratch assay. As a result, the purified protein induced cell proliferation and the EC50 value of protein based drug candidate was 0.051 mM. Additionally, it was determined that the migration ability of the HUVECs was promoted by rhG-CSF in a concentration-dependent manner by in vitro scratch assay.

Keywords

Granulocyte colony stimulating factor,recombinant proteins,angiogenesis,cell proliferation

Supporting Institution

Tokat Gaziosmanpasa University Scientific Research Project

Project Number

2016/61

Thanks

This study was financially supported by the Tokat Gaziosmanpasa University Scientific Research Project (2016/61). Additionally, Yasemin BOZKURT was supported by TUBİTAK-BİDEB 2210/C National Scholarship Program for MSc students.

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Details

Primary Language

English

Subjects

Chemical Engineering

Journal Section

Research Article

Authors

Yasemin Bozkurt This is me
0000-0002-3456-3261
Türkiye

Sema Bilgin
0000-0001-5921-5434
Türkiye

Seçil Erden
0000-0001-8473-5896
Türkiye

İsmail Furkan Turan
0000-0002-2391-1582
Türkiye

İsa Gökçe This is me
0000-0002-5023-9947
Türkiye

Publication Date

December 31, 2019

Submission Date

August 5, 2019

Acceptance Date

September 20, 2019

Published in Issue

Year 2019 Volume: 3 Number: 2

DOI

https://doi.org/10.32571/ijct.600180

IZ

https://izlik.org/JA84NT99CS

Cite

RIS / Bibtex

APA

Bozkurt, Y., Bilgin, S., Erden, S., Turan, İ. F., & Gökçe, İ. (2019). Recombinant human G-CSF production as a protein based drug candidate for hematology and oncology. International Journal of Chemistry and Technology, 3(2), 92-100. https://doi.org/10.32571/ijct.600180

AMA

1.Bozkurt Y, Bilgin S, Erden S, Turan İF, Gökçe İ. Recombinant human G-CSF production as a protein based drug candidate for hematology and oncology. Int. J. Chem. Technol. 2019;3(2):92-100. doi:10.32571/ijct.600180

Chicago

Bozkurt, Yasemin, Sema Bilgin, Seçil Erden, İsmail Furkan Turan, and İsa Gökçe. 2019. “Recombinant Human G-CSF Production As a Protein Based Drug Candidate for Hematology and Oncology”. International Journal of Chemistry and Technology 3 (2): 92-100. https://doi.org/10.32571/ijct.600180.

EndNote

Bozkurt Y, Bilgin S, Erden S, Turan İF, Gökçe İ (December 1, 2019) Recombinant human G-CSF production as a protein based drug candidate for hematology and oncology. International Journal of Chemistry and Technology 3 2 92–100.

IEEE

[1]Y. Bozkurt, S. Bilgin, S. Erden, İ. F. Turan, and İ. Gökçe, “Recombinant human G-CSF production as a protein based drug candidate for hematology and oncology”, Int. J. Chem. Technol., vol. 3, no. 2, pp. 92–100, Dec. 2019, doi: 10.32571/ijct.600180.

ISNAD

Bozkurt, Yasemin - Bilgin, Sema - Erden, Seçil - Turan, İsmail Furkan - Gökçe, İsa. “Recombinant Human G-CSF Production As a Protein Based Drug Candidate for Hematology and Oncology”. International Journal of Chemistry and Technology 3/2 (December 1, 2019): 92-100. https://doi.org/10.32571/ijct.600180.

JAMA

1.Bozkurt Y, Bilgin S, Erden S, Turan İF, Gökçe İ. Recombinant human G-CSF production as a protein based drug candidate for hematology and oncology. Int. J. Chem. Technol. 2019;3:92–100.

MLA

Bozkurt, Yasemin, et al. “Recombinant Human G-CSF Production As a Protein Based Drug Candidate for Hematology and Oncology”. International Journal of Chemistry and Technology, vol. 3, no. 2, Dec. 2019, pp. 92-100, doi:10.32571/ijct.600180.

Vancouver

1.Yasemin Bozkurt, Sema Bilgin, Seçil Erden, İsmail Furkan Turan, İsa Gökçe. Recombinant human G-CSF production as a protein based drug candidate for hematology and oncology. Int. J. Chem. Technol. 2019 Dec. 1;3(2):92-100. doi:10.32571/ijct.600180

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