In this study, in order
to obtain fructose syrup from starch, pullulanase (PUL), glucoamylase (GA) and
glucose isomerase (GI) were immobilized together with cross-linked enzyme
aggregates (CLEAs), and named as 'combi-CLEAs'. The values of Vmaxand Kmfrom
the kinetic parameters of combi-CLEAs [(GA + PUL) + GI] were found as 12 U g-1 and 0.1157 mg ml-1
in optimum conditions, respectively. After
50 uses, it was observed that the remaining activity of combi-CLEAs [GA + PUL +
GI] was preserved at 46%. The thermal stability of combi-CLEAs
[GA + PUL) + GI] was observed to be 44% and 30%, respectively, at 60 and 80 ° C
after 24 hours. At the end of 30 days, the
storage stabilities of combi-CLEAs [(GA + PUL) + GI] was determined as 56% and
13% at the temperatures of 4 and 25°C, respectively.
Birincil Dil | İngilizce |
---|---|
Konular | Kimya Mühendisliği |
Bölüm | Makale |
Yazarlar | |
Yayımlanma Tarihi | 27 Haziran 2018 |
Yayımlandığı Sayı | Yıl 2018 Cilt: 2 Sayı: 1 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.