Evaluation of Antioxidant, Antiproliferative and Anticholinesterase Activities of Iberis carnosa Willd

Year 2025, Volume: 9 Issue: 1, 91 - 96, 24.06.2025

İmran Uysal

https://doi.org/10.32571/ijct.1668024

Abstract

This study investigated the bioactivity of Iberis carnosa Willd, a plant known for its diverse natural compound. Utilizing Soxhlet extraction, an ethanol extract was prepared and subsequently analyzed for antioxidant, antiproliferative, and anticholinesterase activities. Antioxidant capacity was quantified using Rel Assay kits, yielding a total antioxidant status (TAS) of 4.489±0.095 mmol/L, a total oxidant status (TOS) of 11.325±0.130 µmol/L, and an oxidative stress index (OSI) of 0.252±0.003. Antiproliferative activity was assessed against the A549 lung cancer cell line, demonstrating a dose-dependent inhibition of cell growth. Anticholinesterase activity was evaluated by measuring acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibition, revealing values of 76.52±1.93 μg/mL for AChE and 119.84±1.50 μg/mL for BChE, respectively. These findings suggest that I. carnosa possesses significant biological activities and warrants further investigation for potential therapeutic applications, particularly in neurodegenerative diseases and cancer.

Keywords

Iberis carnosa , antioxidant activity , antiproliferative effect , anticholinesterase activity , A549 cell line , biological activity

Ethical Statement

There is no Ethical Statement.

Supporting Institution

There is no Supporting Institution

Project Number

There is no Project Number.

Thanks

Associate Professor Mustafa SEVİNDİK

References

• 1. Sevindik, M.; Yazar, M.; Polat, A. O.; Karatepe, H. K.; Uysal, İ.; Özdemir, B.; Uysal, N. Indian Journal of Natural Products and Resources, 2024,15, 473-490.
• 2. Abdel-Aziz, S. M.; Aeron, A.; Kahil, T. A. Microbes in food and health, 2016, 97-116.
• 3. Mohammed, F. S.; Uysal, I.; Sevindik, M. Prospects in Pharmaceutical Sciences, 2023, 21, 1-21.
• 4. Sevindik, M.; Mohammed, F.S.; Uysal, I. Prospects in Pharmaceutical Sciences, 2023, 21, 38-48.
• 5. Uysal, N.; Uysal, I.; Koçer, O.; Şabik, A. E.; Kaya, H.; Yaz, H. H. Eurasian Journal of Medical and Biological Sciences, 2025, 5, 40-57.
• 6. Mohammed, F. S.; Sevindik, M.; Uysal, İ.; Çesko, C.; Koraqi, H. Measurement: Food, 2024, 100157.
• 7. Sabik, A. E.; Sevindik, M.; Mohammed, F. S.; Dogan, M. Pharmaceutical Chemistry Journal, 2024, 58.
• 8. Yazar, M.; Sevindik, M.; Polat, A. O.; Koçer, O.; Karatepe, H. K.; Uysal, İ. Prospects in Pharmaceutical Sciences, 2024, 22, 146-154.
• 9. Ahmed, A.; Shuaib, M.; Shirsat, M. K.; Sharma, D. Indonesian Journal of Pharmaceutical and Clinical Research, 2023, 6, 13-24.
• 10. Mamun, A. A.; Khan, M. S. S. Australian Herbal Insight, 2020, 3, 1-9.
• 11. Oskay, D. Biological Diversity and Conservation, 2016, 9, 188-192.
• 12. Çilden, E. Nordic Journal of Botany, 2022, e03671.
• 13. Çilden, E.; Zare, G. Hacettepe Journal of Biology and Chemistry, 2019,47, 389-401.
• 14. Erel O. Clinical biochemistry, 2004,37, 277-285.
• 15. Erel O. Clinical biochemistry, 2005, 38, 1103-1111.
• 16. Sevindik, M. Fresenius Environmental Bulletin, 2019, 28, 3713-3717.
• 17. Ellman, G. L.; Courtney, K. D.; Andres Jr, V.; Featherstone, R. M. Biochemical pharmacology, 1961, 7, 88-95. 18. Bal, C.; Akgul, H.; Sevindik, M.; Akata, I.; Yumrutas, O. Fresenius Environmental Bulletin, 2017, 26, 6246-6252.
• 19. Sevindik, M.; Akgul, H.; Dogan, M.; Akata, I.; Selamoglu, Z. Fresenius environmental Bulletin, 2018, 27, 1946-1952.
• 20. Mishra, N.; Jiang, C.; Chen, L.; Paul, A.; Chatterjee, A.; Shen, G. Frontiers in Plant Science, 2023,14, 1110622.
• 21. Vicidomini, C.; Palumbo, R.; Moccia, M.; Roviello, G. N. Journal of Xenobiotics, 2024, 14, 1541-1569.
• 22. Hasanuzzaman, M.; Bhuyan, M. B.; Zulfiqar, F.; Raza, A.; Mohsin, S. M.; Mahmud, J. A.; Fotopoulos, V. Antioxidants, 2020, 9, 681.
• 23. Schempp, H.; Totha, A.; Weiser, D.; Elstner, E. F. Arzneimittelforschung, 2003, 53, 568-577.
• 24. Golkar, P.; Moattar, F. Natural Product Communications, 2019, 14, 1934578X19846355.
• 25. Unal, O.; Eraslan, E. C.; Uysal, I.; Mohammed, F. S.; Sevindik, M.; Akgul, H. Fresenius Environmental Bulletin, 2022, 31, 7341-7346.
• 26. Gürgen, A.; Sevindik, M.; Krupodorova, T.; Uysal, I.; Unal, O. BMC biotechnology, 2024, 24, 83.
• 27. Sevindik, M.; Gürgen, A.; Krupodorova, T.; Uysal, İ.; Koçer, O. Scientific Reports, 2024, 14, 31403.
• 28. Uysal, I.; Sevindik, M.; Dogan, M.; Mohammed, F. S.; Seğmenoğlu, M. S.; Pehlivan, M. Pharmaceutical Chemistry Journal, 2024, 58, 1289-1297.
• 29. Gürgen, A.; Sevindik, M. Journal of Food Processing and Preservation, 2022, 46, e16949.
• 30. Uysal, İ. International Journal of Chemistry and Technology, 2022, 6, 93-96.
• 31. Santos, T. C. D.; Gomes, T. M.; Pinto, B. A. S.; Camara, A. L.; Paes, A. M. D. A. Frontiers in Pharmacology, 2018, 9, 1192.
• 32. Gülçin, İ.; Gören, A. C.; Taslimi, P.; Alwasel, S. H.; Kılıc, O.; Bursal, E. Biocatalysis and agricultural biotechnology, 2020, 23, 101441.
• 33. Murray, A. P.; Biscussi, B.; Cavallaro, V.; Donozo, M.; Rodriguez, S. A. Current Neuropharmacology, 2024, 22, 1621-1649.
• 34. Jamal, Q. M. S.; Khan, M. I.; Alharbi, A. H.; Ahmad, V.; Yadav, B. S. Nutrients, 2023,15, 1579. 35. Sevindik, M.; Akgul, H.; Selamoglu, Z.; Braidy, N. Oxidative medicine and cellular longevity, 2020, 5620484.
• 36. Alzahrani, A. R.; Hosny, N.; Mohamed, D. I.; Nahas, H. H. A.; Albogami, A.; Al-Hazani, T. M. I.; Saied, E. M. RSC advances, 2024, 14, 19400-19427.
• 37. Bada, L.; Pereira, R. B.; Pereira, D. M.; Lores, M.; Celeiro, M.; Quezada, E.; Viña, D. Molecules, 2023, 28, 351.