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Bioactive Content, Antioxidant Capacity, and Quality Assessment of Origanum onites and Thymus fallax (Lamiaceae)

Year 2026, Volume: 10 Issue: 1, 1 - 9, 13.02.2026
https://izlik.org/JA43DT56SH

Abstract

Lamiaceae family includes approximately 236 genera and 6900 to 7200 species. Medicinal plants belonging to this family have been used for many years in the treatment of various animal and human diseases based on their antibacterial, antioxidant, antitumoral, antifungal, analgesic and insecticidal effects.This research covers the determination of quality control methods and analytical studies for Thymus fallax (catri) and Origanum onites (kekik) which were collected from Şırnak and Izmir, respectively. Heavy metal analyses using Wavelength Dispersive X-Ray Spectrometer (WDXRF) method, determination of ash content and loss on drying according to the European Pharmacopoeia 8.0 standards were carried out. Furthermore, the total phenolic contents of aqueous extracts of both plants were tested with the Folin-Ciocalteu colorimetric method to getting first step for determining their antioxidant activities. According to WDXRF results, the amounts of heavy metals determined were within the limits of pharmacopoeia standards for both species. In addition, total ash and water amounts were determined as 7%, 14% and 14%, 16% for Origanum onites and Thymus fallax respectively and were determined to be in compliance with pharmacopoeia standards. Total phenolic contents (GAE µg/mL) were found to be 28.152 ± 0.389 and 41.009 ± 2.059 for Origanum onites and Thymus fallax, respectively. This is an evidence that both species contain phenolic compounds that will cause them to display antioxidant activity. The data obtained within the scope of the study showed that if the plants are procured correctly and the water extracts are prepared with appropriate methods, the quality and effectiveness will increase the efficiency.

Project Number

T6572200

Thanks

This study was supported by the Scientific and Technological Research Council of Türkiye (TÜBİTAK) under the 2209-A Undergraduate Research Projects Support Program (Project No: T6572200).

References

  • Baser, K. H. C. (2008). Biological and pharmacological activities of carvacrol and carvacrol bearing essential oils. Current Pharmaceutical Design, 14 (29), 3106-3119. https://doi.org/10.2174/138161208786404227
  • Boros, B., Jakabová, S., Dörnyei, Á., Horváth, G., Pluhár, Z., Kilár, F., & Felinger, A. (2010). Determination of polyphenolic compounds by liquid chromatography–mass spectrometry in Thymus species. Journal of Chromatography A, 1217 (51), 7972–7980. https://doi.org/10.1016/j.chroma.2010.10.010
  • Cemeroğlu B. (2019). Gıda Analizleri. Tlb Yayınları, 18-23.
  • Dai, J., & Mumper, R. J. (2010). Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules, 15 (10), 7313–7352. https://doi.org/10.1016/j.chroma.2010.07.042
  • Dapkevicius, A., Venskutonis, R., van Beek, T. A., & Linssen, J. P. (1998). Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. Journal of the Science of Food and Agriculture, 77 (1), 140-146. https://doi.org/10.1002/(SICI)1097-0010(199805)77
  • Davis, P. H (Ed.). (1982). Flora of Turkey and the East Aegean Islands (Vol. 7). Edinburgh UK.: Edinburgh University Press.
  • Davis, P. H., Mill, R. R., & Tan, K. (1988). Flora of Turkey and the East Aegean Islands (Vol. 10, Suppl.). Edinburgh, UK: Edinburgh University Press.
  • Duman, H., Güner, A., & Özhatay, N. (1995). Origanum L. In A. Güner, N. Özhatay, T. Ekim, & K. H. C. Baser (Eds.), Flora of Turkey and the East Aegean Islands (Vol. 11). Edinburgh, UK: Edinburgh University Press., pp. 207–236.
  • European Directorate for the Quality of Medicines & HealthCare (EDQM) (2013). European Pharmacopoeia. 8th ed. Strasbourg: Council of Europe.
  • Güner, A., Aslan, S., Ekim, T., Vural, M. & Babaç, M. T. (Edlr.), (2012). Türkiye Bitkileri Listesi (Damarlı Bitkiler). İstanbul: Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını.
  • Hossain, M. B., Rai, D. K., Brunton, N. P., Martin-Diana, A.B., & Barry-Ryan, C. (2010). Characterization of phenolic composition in Lamiaceae spices by LC-ESIMS/MS. Journal of Agricultural and Food Chemistry, 58 (19), 10576-10581. https://doi.org/10.1021/jf102042g
  • Karagözlü, C., & Akbulut, N. (2012). Laboratuvar Tekniği. İzmir: Ege Üniversitesi Ziraat Fakültesi Yayınları, 143.
  • Kulisic, T., Radonic, A., & Milos, M. (2005). Antioxidant properties of thyme (Thymus vulgaris L.) and wild thyme (Thymus serpyllum L.) essential oils. Italian Journal of Food Science, 17 (3), 315-324.
  • Marchese, A., Orhan, I. E., Daglia, M., Barbieri, R., Di Lorenzo, A., Nabavi, S. F., & Nabavi, S. M. (2016). Antibacterial and antifungal activities of thymol: A brief review of the literature. Food Chemistry, 210, 402-414. https://doi.org/10.1016/j.foodchem.2016.04.111
  • Marguí, E., Dalipi, R., Sangiorgi, E., Bival Štefan, M., Sladonja, K., Rogga, V., & Jablan, J. (2022). Determination of essential elements (Mn, Fe, Cu and Zn) in herbal teas by TXRF, FAAS and ICP‐OES. X‐Ray Spectrometry, 51 (3), 204-213. https://doi.org/10.1002/xrs.3241
  • Morales, R. (2002). The history, botany and taxonomy of the genus Thymus. In E. Stahl-Biskup & F. Sáez (Eds.), Thyme: The genus Thymus London, UK: Taylor & Francis, pp. 1–43.
  • Moshari-Nasirkandi, A., Alirezalu, A., Alipour, H., & Amato, J. (2023). Screening of 20 species from Lamiaceae family based on phytochemical analysis, antioxidant activity and HPLC profiling. Scientific Reports, 13 (1), 16987. https://doi.org/10.1038/s41598-023-44337-7
  • Özcan, M. M., & Akbulut, M. (2008). Estimation of minerals, nitrate and nitrite contents of medicinal and aromatic plants. Food Chemistry, 106 (2), 852–858. https://doi.org/10.1016/j.foodchem.2007.06.045
  • Rice-Evans, C. A., Miller, N. J., & Paganga, G. (1997). Antioxidant properties of phenolic compounds. Trends in Plant Science, 2 (4), 152–159. https://doi.org/10.1016/S1360-1385(97)01018-2
  • Singleton, V. L, & Rossi, J. A. (1965). Colorimetry of Total Phenolic Compounds with Phosphomolybdic-Phosphotungstic Acid Reagents. American Journal of Enology and Viticulture, 16, 144-158. . https://doi.org/10.5344/ajev.1965.16.3.144
  • Skendi, A., Irakli, M., & Chatzopoulou, P. (2017). Analysis of phenolic compounds in Greek plants of Lamiaceae family by HPLC. Journal of Applied Research On Medicinal And Aromatic Plants, 6, 62-69. https://doi.org/10.1016/j.jarmap.2017.02.001
  • Skoog, D.A., Holler, F.J., & Crouch, S. R. (2014). Principles of Instrumental Analysis. 6th ed. Belmont (CA): Cengage Learning.
  • Spyridopoulou, K., Fitsiou, E., Bouloukosta, E., Tiptiri-Kourpeti, A., Vamvakias, M., Oreopoulou, A., & Chlichlia, K. (2019). Extraction, chemical composition and anticancer potential of Origanum onites L. essential oil. Molecules, 24 (14), 2612. https://doi.org/10.3390/molecules24142612
  • Stahl-Biskup E. (2002). Thyme as a Herbal Drug-Pharmacopoeias and Other Product Characteristics, In: Stahl-Biskup and Saez (Eds.) The genus Thymus. London: Taylor & Francis, 29. https://doi.org/10.4324/9780203216859
  • Tepe, B., Cakir, A., & Tepe, A. S. (2016). Medicinal uses, phytochemistry, and pharmacology of Origanum onites (L.): A Review. Chemistry & Biodiversity, 13 (5), 504-520. https://doi.org/10.1002/cbdv.201500069
  • Tzima, K., Brunton, N. P., & Rai, D. K. (2018). Qualitative and quantitative analysis of polyphenols in Lamiaceae plants- A review. Plants, 7 (25), 1-30. https://doi.org/10.3390/plants7020025
  • World Health Organization. (2007). WHO guidelines for assessing quality of herbal medicines with reference to contaminants and residues. WHO Press.
  • Yetim, H. (2001). Gıda Analizleri (Ders Notu), Erzurum: Atatürk Üniversitesi Ziraat Fakültesi Ofset Tesisi, 161.
There are 28 citations in total.

Details

Primary Language English
Subjects Botany (Other), Medicinal Plants
Journal Section Research Article
Authors

Pelin Taştan 0000-0003-0913-5369

Ayten Acar 0009-0003-1413-2741

Project Number T6572200
Submission Date January 13, 2026
Acceptance Date February 10, 2026
Publication Date February 13, 2026
IZ https://izlik.org/JA43DT56SH
Published in Issue Year 2026 Volume: 10 Issue: 1

Cite

APA Taştan, P., & Acar, A. (2026). Bioactive Content, Antioxidant Capacity, and Quality Assessment of Origanum onites and Thymus fallax (Lamiaceae). International Journal of Nature and Life Sciences, 10(1), 1-9. https://izlik.org/JA43DT56SH

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