Determination of Calcium Ion in Environmental Samples Using a Potentiometric Sensor as a Detector in Flow Injection Analysis Method

Yıl 2019, , 37 - 45, 01.07.2019

Adem Asan

https://doi.org/10.29132/ijpas.500387

Öz

In this
study, Ca²⁺-selective potentiometric PVC-membrane electrode was
developed with the commercially purchased ionophore substance to be used to
determine calcium concentration in water samples. The potentiometric
performance characteristics of the electrode were determined and their
suitability for environmental analysis was investigated The measurements taken
with the Ca²⁺-selective electrode indicated an average potential
change of 23,5 mV per decade against the main ion concentrations. The electrode
showed linear behavior against the main ion solution in the concentration range
between 1×10^-1-1×10^-5
mol L^-1, and it was found to be highly selective against the main
ion besides the alkali and alkaline earth metal ions. Micro dead volume flow
cells were prepared in the laboratory and the calcium determination was made in
environmental water samples by using the home-made electrode as a detector in
the flow injection analysis (FIA) system. In the flow-injection analysis system
(FIA), 5×10^-5
M CaSO₄ ve MgSO₄; 1×10^-6 M NaCl, KNO₃,
LiNO₃ ve NH₄Cl solution mixture was used as the mobile phase. In this system, the flow-rate was
set to 1,0 mL min^-1 and injection volume 20 μL. In addition, calcium
determination in water samples was performed by standard addition method and
all measurements were compared with potentiometric results. The results show
that the Ca²⁺-selective micro-potentiometric PVC-membrane electrode
can effectively be used for routine determination of calcium ion in water in
environmental samples.

Anahtar Kelimeler

Flow injection analysis, environmental sample, calcium, PVC-membrane electrode

Akış Enjeksiyon Analiz Yönteminde Dedektör Olarak Potansiyometrik Sensör Kullanarak Çevre Numunelerinde Kalsiyum İyonu Tayini

Öz

Bu
çalışmada, su numunelerindeki kalsiyum derişiminin belirlenmesinde kullanılmak
üzere ticari olarak satın alınan iyonofor madde ile Ca²⁺-seçici
mikro potansiyometrik PVC-membran elektrot geliştirildi. Elektrotun
potansiyometrik performans karakteristikleri belirlendi ve çevresel analizlere
uygunluğu araştırıldı. Hazırlanan Ca²⁺-seçici elektrot ile alınan
ölçümlerde ana iyon çözeltisine karşı her 10 katlık kalsiyum iyonu konsantrasyonu
değişimi için ortalama 23,5 mV’luk bir potansiyel farkı gözlendi. Elektrotun, 1×10^-1-1×10^-5
mol L^-1 derişim aralığında ana iyon çözeltisine karşı doğrusal
davranış sergilediği, alkali ve toprak alkali metal iyonlarının yanında ana
iyona karşı oldukça seçici olduğu belirlendi. Laboratuvarda mikro ölü hacme
sahip akış hücreleri hazırlandı ve akış enjeksiyon analizi (AEA) sisteminde hareketli
faz olarak 5×10^-5 M CaSO₄ ve MgSO₄; 1×10^-6
M NaCl, KNO₃, LiNO₃ ve NH₄Cl çözelti karışımı
kullanılmış ve akış hızı: 1,0 mL dk^-1, enjeksiyon hacmi ise 20 μL olarak
belirlenmiştir. Geliştirilen bu elektrotların AEA sisteminde dedektör olarak
kullanılması ile çevresel su numunelerinde kalsiyum iyonu tayini gerçekleştirildi.
Ayrıca, su numunelerindeki kalsiyum tayini standart ekleme yöntemi ile de yapıldı
ve tüm ölçümler potansiyometrik sonuçlarla karşılaştırmalı olarak verildi. Elde
edilen sonuçlar, geliştirilen Ca²⁺-seçici mikro potansiyometrik
PVC-membran elektrotun çevre numunelerindeki sularda kalsiyum iyonunun rutin
tayininde etkin bir şekilde kullanılabileceğini göstermektedir.

Anahtar Kelimeler

Akış enjeksiyon analiz, çevre numunesi, kalsiyum, PVC-membran elektrot

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